Internalization and intracellular trafficking of nanoparticles: biological activity and nanotoxicological profile

• Grant number: 14/03002-7
• Support Opportunities: Research Grants - Young Investigators Grants
• Start date: March 01, 2015
• End date: February 29, 2020
• Field of knowledge: Biological Sciences - Biochemistry - Enzymology
• Principal Investigator: Marcelo Bispo de Jesus
• Grantee: Marcelo Bispo de Jesus
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated research grant(s): 15/06134-4 - Multi-User Equipment approved in grant 2014/03002-7: cell imaging multi-mode reader, AP.EMU
• Associated scholarship(s): 17/10249-7 - Molecular mechanisms triggered by solid lipid nanoparticles in prostate cells PNT2 and PC-3: evaluation of the TGF-Beta pathway, BP.IC; 16/16259-1 - Bioinspired gene delivery optimization of nanoparticles in retinal pigmented epithelium model, BP.MS; 16/03765-6 - In vitro and in vivo determination of toxicological profile of nanoformulations, BP.PD; 15/05026-3 - Internalization And Intracellular Trafficking of Nanoparticles: Biological Activity and Nanotoxicological Profile, BP.JP

Abstract

Nanomedicine is presented as a new technology to assist in the treatment and diagnosis of various diseases. However, this new technology brings new challenges, not only in developing new nanodevices, as well as in the interaction of these nanomaterials with biological environment. Understanding the molecular mechanisms involved in the internalization and processing of nanoparticles by eukaryotic cells is vital to develop more efficient and safer nanoparticles. Although the more internalization pathways lead to degradation of internalized material, increasing evidence in the literature suggest that some nanomaterials are biopersistent, thus leading to negative impact on cellular metabolism. Thereby, recently, a new area of knowledge has been established, the nanotoxicology. This project aims to study how the internalization and intracellular trafficking of nanoparticles affects their biological role, e.g. genes delivery, as well as evaluating the adverse effects caused by these nanomaterials at the cellular level. (AU)

Scientific publications

(16)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GARCIA-FOSSA, FERNANDA; GAAL, VLADIMIR; DE JESUS, MARCELO BISPO. PyScratch: An ease of use tool for analysis of scratch assays. COMPUTER METHODS AND PROGRAMS IN BIOMEDICINE, v. 193, SEP 2020. (14/03002-7, 15/06134-4)

FACHI, JOSE LUIS; FELIPE, JAQUELINE DE SOUZA; PRAL, LAIS PASSARIELLO; DA SILVA, BRUNA KARADI; CORREA, RENAN OLIVEIRA; PEREIRA DE ANDRADE, MIRELLA CRISTINY; DA FONSECA, DENISE MORAIS; BASSO, PAULO JOSE; SARAIVA CAMARA, NIELS OLSEN; DE SALES E SOUZA, ERICKA LORENNA; et al. Butyrate Protects Mice from Clostridium difficile-Induced Colitis through an HIF-1-Dependent Mechanism. CELL REPORTS, v. 27, n. 3, p. 750+, APR 16 2019. (17/01451-7, 12/10653-9, 16/23142-3, 13/06810-4, 14/03002-7, 15/01507-7, 18/01753-6, 15/06134-4, 17/25679-7, 17/06577-9, 18/02208-1, 17/26366-2, 17/16280-3)

GUILHERME, VIVIANE A.; RIBEIRO, LIGIA N. M.; TOFOLI, GIOVANA RADOMILLE; FRANZ-MONTAN, MICHELLE; DE PAULA, ENEIDA; DE JESUS, MARCELO BISPO. Current Challenges and Future of Lipid Nanoparticles Formulations for Topical Drug Application to Oral Mucosa, Skin, and Eye. CURRENT PHARMACEUTICAL DESIGN, v. 23, n. 43, p. 6659-6675, 2017. (14/03002-7, 14/14457-5)

ALLAN RADAIC; GUSTAVO OLIVEIRA PUGLIESE; GUILHERME CAPIRAÇO CAMPESE; FRANCISCO BENEDITO TEIXEIRA PESSINE; MARCELO BISPO DE JESUS. COMO ESTUDAR INTERAÇÕES ENTRE NANOPARTÍCULAS E SISTEMAS BIOLÓGICOS. Química Nova, v. 39, n. 10, p. 1236-1244, 2016-12-00. (14/03002-7, 13/05432-6)

MENDONCA, M. C. P.; RADAIC, A.; FOSSA, F. G.; CRUZ-HOFLING, M. A.; DE JESUS, M. B.; IOP. Toxicity of cationic solid lipid nanoparticles in rats. WAKE CONFERENCE 2021, v. 1323, p. 6-pg., 2019-01-01. (14/03002-7, 16/03765-6)

BANDEIRA FERREIRA, LUIZ ALBERTO; GARCIA-FOSSA, FERNANDA; RADAIC, ALLAN; DURAN, NELSON; FAVARO, WAGNER JOSE; DE JESUS, MARCELO BISPO. Biogenic silver nanoparticles: In vitro and in vivo antitumor activity in bladder cancer. EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS, v. 151, p. 162-170, JUN 2020. (15/06134-4, 14/03002-7)

PADILHA MENDONCA, MONIQUE CULTURATO; FERREIRA, LUIZ BANDEIRA; RIZOLI, CINTIA; BATISTA, ANGELA GIOVANA; MAROSTICA JUNIOR, MARIO ROBERTO; DA SILVA, EMANUELI DO NASCIMENTO; CADORE, SOLANGE; DURAN, NELSON; DA CRUZ-HOFLING, MARIA ALICE; DE JESUS, MARCELO BISPO. N-Acetylcysteine reverses silver nanoparticle intoxication in rats. Nanotoxicology, v. 13, n. 3, p. 326-338, MAR 16 2019. (14/03002-7, 16/03765-6, 15/06134-4)

MENDONCA, MONIQUE C. P.; RODRIGUES, NATALIA P.; DE JESUS, MARCELO B.; AMORIM, MONICA J. B.. Graphene-Based Nanomaterials in Soil: Ecotoxicity Assessment Using Enchytraeus crypticus Reduced Full Life Cycle. NANOMATERIALS, v. 9, n. 6, JUN 2019. (14/03002-7, 17/18867-1)

FERREIRA, LUIZ A. B.; DOS REIS, SAMARA BONESSO; DA SILVA, EMANUELI DO NASCIMENTO; CADORE, SOLANGE; BERNARDES, JULIANA DA SILVA; DURAN, NELSON; DE JESUS, MARCELO B.. Thiol-antioxidants interfere with assessing silver nanoparticle cytotoxicity. Nanomedicine-Nanotechnology Biology and Medicine, v. 24, FEB 2020. (15/06134-4, 14/03002-7)

DOS REIS, SAMARA BONESSO; SILVA, JULIANA DE OLIVEIRA; GARCIA-FOSSA, FERNANDA; LEITE, ELAINE AMARAL; MALACHIAS, ANGELO; POUND-LANA, GWENAELLE; FURTADO MOSQUEIRA, VANESSA CARLA; OLIVEIRA, MONICA CRISTINA; BRANCO DE BARROS, ANDRE LUIS; DE JESUS, MARCELO BISPO. Mechanistic insights into the intracellular release of doxorubicin from pH-sensitive liposomes. BIOMEDICINE & PHARMACOTHERAPY, v. 134, FEB 2021. (14/03002-7, 15/06134-4)

MENDONCA, MONIQUE C. P.; RODRIGUES, NATALIA P.; SCOTT-FORDSMAND, JANECK J.; DE JESUS, MARCELO BISPO; AMORIM, MONICA J. B.. The toxicity of silver nanomaterials (NM 300K) is reduced when combined with N-Acetylcysteine: Hazard assessment on Enchytraeus crypticus. Environmental Pollution, v. 256, JAN 2020. (14/03002-7, 17/18867-1)

BECHER, TIAGO B.; MENDONCA, MONIQUE C. P.; DE FARIAS, MARCELO A.; PORTUGAL, V, RODRIGO; DE JESUS, MARCELO B.; ORNELAS, CATIA. Soft Nanohydrogels Based on Laponite Nanodiscs: A Versatile Drug Delivery Platform for Theranostics and Drug Cocktails. ACS APPLIED MATERIALS & INTERFACES, v. 10, n. 26, p. 21891-21900, JUL 4 2018. (16/03765-6, 13/11519-7, 14/03002-7, 18/02093-0)

RADAIC, A.; DE JESUS, M. B.. Solid lipid nanoparticles release DNA upon endosomal acidification in human embryonic kidney cells. Nanotechnology, v. 29, n. 31, AUG 3 2018. (14/03002-7, 15/06134-4)

SAMPAIO BONAFE, CARLOS FRANCISCO; CONCEICAO BISPO, JOSE AILTON; DE JESUS, MARCELO BISPO. The Polygonal Model: A Simple Representation of Biomolecules as a Tool for Teaching Metabolism. BIOCHEMISTRY AND MOLECULAR BIOLOGY EDUCATION, v. 46, n. 1, p. 66-75, JAN-FEB 2018. (14/03002-7)

MENDONCA, MONIQUE C. P.; RIZOLI, CINTIA; AVILA, DAIANA S.; AMORIM, MONICA J. B.; DE JESUS, MARCELO B.. Nanomaterials in the Environment: Perspectives on in Vivo Terrestrial Toxicity Testing. FRONTIERS IN ENVIRONMENTAL SCIENCE, v. 5, 2017. (14/03002-7, 16/03765-6)

BANDEIRA FERREIRA, LUIZ ALBERTO; GARCIA-FOSSA, FERNANDA; RADAIC, ALLAN; DURAN, NELSON; FAVARO, WAGNER JOSE; DE JESUS, MARCELO BISPO. Biogenic silver nanoparticles: In vitro and in vivo antitumor activity in bladder cancer. EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS, v. 151, p. 9-pg., 2020-06-01. (15/06134-4, 14/03002-7)

Filed patent(s) as a result of this research project