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The relationship between glucocorticoid receptor activation and the neuronal hyperexcitability in the basolateral amygdala in the restraint stress-induced long-lasting anxiety and their implications in the impaired contextual fear extinction

Grant number: 16/03572-3
Support type:Regular Research Grants
Duration: August 01, 2016 - October 31, 2018
Field of knowledge:Biological Sciences - Pharmacology
Principal Investigator:Carolina Demarchi Munhoz
Grantee:Carolina Demarchi Munhoz
Home Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Assoc. researchers: Ki Ann Goosens

Abstract

Anxiety-related disorders are common among psychiatric patients and are widely present in studies devoted to stress. In addition to the long-lasting anxiety behavior, impaired extinction of learned fear is associated with the development of post-traumatic stress disorder, one of the most debilitating anxiety-related disorder. The amygdala is a key brain structure essential for behavioral manifestations of emotional content and electrical stimulation of the basolateral amygdala (BLA) promotes anxiety-like behavior in rats. Because the complexity of such phenomena, it is urgent the need to understand the correlation among stress, BLA neuronal excitability, and anxiety; and how the activation of glucocorticoid receptors intracellular signaling coordinates these events and modulates the extinction of learned fear. These mechanisms are particularly relevant to the understanding of pathologies related to fear and anxiety. Thus, the objective of this project is, using viral vectors to modulate GR activity (HSV-GR and dnGR-HSV) and the neuronal excitability in BLA (SK2-HSV), to verify whether both restraint stress-induced long-lasting anxiety and the impairment in contextual fear extinction are dependent on the increase of neuronal excitability in the BLA and/or nuclear activity of GR. We feel that this project brings major advances in the understanding of stress-associated disorders, and can offer structural and molecular substrates as therapeutic targets that will serve as basis for the cure of such malady, such as the posttraumatic stress disorder. (AU)

Scientific publications (5)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DOS SANTOS, NILTON; NOVAES, LEONARDO S.; DRAGUNAS, GUILHERME; RODRIGUES, JENNIFER R.; BRANDAO, WESLEY; CAMARINI, ROSANA; SCHATZMANN PERON, JEAN PIERRE; DEMARCHI MUNHOZ, CAROLINA. High dose of dexamethasone protects against EAE-induced motor deficits but impairs learning/memory in C57BL/6 mice. SCIENTIFIC REPORTS, v. 9, APR 30 2019. Web of Science Citations: 1.
NOVAES, LEONARDO SANTANA; DOS SANTOS, NILTON BARRETO; PERFETTO, JULIANO GENARO; GOOSENS, KI ANN; MUNHOZ, CAROLINA DEMARCHI. Environmental enrichment prevents acute restraint stress-induced anxiety-related behavior but not changes in basolateral amygdala spine density. PSYCHONEUROENDOCRINOLOGY, v. 98, p. 6-10, DEC 2018. Web of Science Citations: 2.
NOVAES, LEONARDO SANTANA; DOS SANTOS, NILTON BARRETO; DRAGUNAS, GUILHERME; PERFETTO, JULIANO GENARO; CARLOS LEZA, JUAN; SCAVONE, CRISTOFORO; MUNHOZ, CAROLINA DEMARCHI. Repeated Restraint Stress Decreases Na,K-ATPase Activity via Oxidative and Nitrosative Damage in the Frontal Cortex of Rats. Neuroscience, v. 393, p. 273-283, NOV 21 2018. Web of Science Citations: 4.
RUSSELL, ASHLEY L.; TASKER, JEFFREY G.; LUCION, ALDO B.; FIEDLER, JENNY; MUNHOZ, CAROLINA D.; WU, TAO-YIAO JOHN; DEAK, TERRENCE. Factors promoting vulnerability to dysregulated stress reactivity and stress-related disease. Journal of Neuroendocrinology, v. 30, n. 10, SI OCT 2018. Web of Science Citations: 6.
NOVAES, LEONARDO S.; DOS SANTOS, NILTON BARRETO; BATALHOTE, RAFAELA F. P.; MALTA, MARILIA BRINATI; CAMARINI, ROSANA; SCAVONE, CRISTOFORO; MUNHOZ, CAROLINA DEMARCHI. Environmental enrichment protects against stress-induced anxiety: Role of glucocorticoid receptor, ERK, and CREB signaling in the basolateral amygdala. Neuropharmacology, v. 113, n. A, p. 457-466, FEB 2017. Web of Science Citations: 21.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.