| Grant number: | 16/24716-3 |
| Support Opportunities: | Regular Research Grants |
| Start date: | March 01, 2017 |
| End date: | August 31, 2019 |
| Field of knowledge: | Biological Sciences - Microbiology - Biology and Physiology of Microorganisms |
| Principal Investigator: | Juliana Pfrimer Falcão |
| Grantee: | Juliana Pfrimer Falcão |
| Host Institution: | Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil |
| City of the host institution: | Ribeirão Preto |
Abstract
Campylobacteriosis is a zoonosis of worldwide distribution with significant repercussions on the level of public health and with a high socioeconomic impact. C. coli and C. jejuni species are common causes of gastroenteritis in humans affecting annually approximately 2.4 million people in the United States and an estimated of 9 million people in Europe. However, the pathogenesis process of this bacterial genus is not fully known and among the few studies performed with this purpose, the majority has been performed for C. jejuni. Specifically in Brazil, the study and isolation of C. coli are not frequent, making it difficult to evaluate the importance of this pathogen in this country. The aims of this project are to comparatively analyze, by phenotypic tests, sequencing of the genome and transcriptome, 50 C. coli strains isolated from humans, animals, the environment and food during 16 years in Brazil. It will be performed for all strains phenotypic tests of temperature fluctuation, resistance to NaCl, acidic and oxidative stress, invasion to epithelial cells, and proliferation and survival in human (U937) and chickens (HD11) macrophages and analysis of the expression of inflammatory cytokine transcripts in CACO-2 cells by RT-qPCR for strains that present significant results in the cited above tests. It will be done the whole genome sequencing (WGS) of 50 C. coli strains of and the transcriptome sequencing (RNA seq) of two strains of C. coli. For the RNA seq assay two strains of clinical and non-clinical origin will be chosen based on the significant differences in the phenotypic tests cited above. The RNAs of these strains will be extracted after tolerance to acid stress and invasion to Caco-2 cells assays. Moreover, the median lethal dose (LD50) of two strains of C. coli will be performed. Due to the paucity of studies involving the C. coli species, studies that aim to elucidate the mechanisms of pathogenicity and virulence are unpublished and of great importance. The results to be obtained should contribute for the better characterization of C. coli isolated from diverse sources during 16 years in Brazil, contributing for a better understanding of the genotypic and phenotypic diversity, pathogenicity and virulence of this pathogen considered of great importance worldwide. (AU)
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