|Support type:||Scholarships in Brazil - Scientific Initiation|
|Effective date (Start):||August 01, 2013|
|Effective date (End):||July 31, 2015|
|Field of knowledge:||Physical Sciences and Mathematics - Chemistry - Physical-Chemistry|
|Principal researcher:||Ana Paula Ramos|
|Grantee:||Marcos Antonio Eufrásio Cruz|
|Home Institution:||Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil|
The modification of metallic surfaces is used aiming the improvement of the surface physical-chemical features of the metals used of different applications, as in the obtainment of new materials for biomedical applications like bone substitution implants. In this special case, the goal of the modification is to improve the affinity of the surfaces by polar substances which help in the growth of minerals over the surfaces generating biomaterials with osteointegration properties. In this project, we propose the building of biomimetic interfaces through the modification of stainless steel and/or titanium surfaces with Langmuir-Blodgett films composed by lipids and layer-by-layer films composed by polyelectrolytes. These matrices are going to be used as templates for CaCO3 growth after their exposition to CO2(g). The bioactivity of the surfaces after the modification will be evaluated through the exposition to the solutions which simulated the concentration of ions present in the human plasma: if the growth of hydroxyapatite is observed on the surfaces, it is and indicative that the surfaces may be used as bone integration systems after implanted. The influence of different matrices on the growth of biominerals will be evaluated. The films will be characterized by scanning electron microscopy and infrared absorption spectroscopy. Moreover, the quantity of lipids, polymers and minerals deposited on the films will be evaluated using quartz crystal microbalance. The surface charge and the wettability of the surfaces will be determined using potential zeta and contact angle measurements, respectively. The crystallinity of the samples will be determined using X-ray diffraction. The best results will be selected to the in vitro culture of bone cells. The final goal of the project is the obtainment of new material potentially applicable as bone substitution implants.