Lipidomics shotgun in giardiasis, meningitis and tuberculosis: the search of diagnostic markers, using revolutionary new techniques of mass spectrometry

• Grant number: 14/00302-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: August 01, 2014
• End date: March 31, 2017
• Field of knowledge: Health Sciences - Medicine - Pathological Anatomy and Clinical Pathology
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Rodrigo Ramos Catharino
• Grantee: Cibele Zanardi Esteves
• Host Institution: Faculdade de Ciências Médicas (FCM). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

Following advances in genomics and proteomics, new fields of "omics" are emerging. The metabolomics course complements studies in the area of genomics and proteomics, however great physical chemistry metabolome diversity leads to a subdivision of metabolites. Thus, lipidomics is currently emerging as a discipline in the interface of biochemistry of lipids, technology and medicine. This expansion of the field of lipidomics can be attributed to analytical advances, particularly in the development of new techniques of mass spectrometry for the characterization and quantification of the different species of lipids present in a lipidome. Lipids play important roles in cell composition among them membrane composition, energy supply and signal transduction, and therefore can be used as biomarkers in metabolic diseases, infectious diseases and degenerative diseases. The aim of this study is the determination of lipid biomarkers present the etiological agents of giardiasis, meningitis and tuberculosis, as well as lipid biomarkers involved in the progression and treatment of meningitis by mass spectrometry and development of diagnostic methods for giardiasis, meningitis and tuberculosis directly from the feaces, cerebrospinal fluid and sputum, respectively. The development of diagnostic methods based on mass spectrometry can provide a rapid and sensitive diagnosis, allowing rapid patient care and reducing the transmission of these diseases (AU)

Scientific publications

(8)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MELO, CARLOS FERNANDO O. R.; DELAFIORI, JEANY; DE OLIVEIRA, DIOGO N.; GUERREIRO, TATIANE M.; ESTEVES, CIBELE Z.; LIMA, ESTELA DE O.; PANDO-ROBLES, VICTORIA; CATHARINO, RODRIGO R.; NETWORK, ZIKA-UNICAMP. Serum Metabolic Alterations upon Zika Infection. FRONTIERS IN MICROBIOLOGY, v. 8, OCT 10 2017. (15/06809-1, 14/00302-0, 11/50400-0, 16/17066-2)

ODIR RODRIGUES MELO, CARLOS FERNANDO; DE OLIVEIRA, DIOGO NOIN; LIMA, ESTELA DE OLIVEIRA; GUERREIRO, TATIANE MELINA; ESTEVES, CIBELE ZANARDI; BECK, RAISSA MARQUES; PADILLA, MARINA AIELLO; MILANEZ, GUILHERME PAIER; ARNS, CLARICE WEIS; PROENCA-MODENA, JOSE LUIZ; et al. A Lipidomics Approach in the Characterization of Zika-Infected Mosquito Cells: Potential Targets for Breaking the Transmission Cycle. PLoS One, v. 11, n. 10, OCT 10 2016. (15/06809-1, 14/00302-0, 11/50400-0, 14/00084-2, 13/11343-6)

ESTEVES, CIBELE ZANARDI; DIAS, LETICIA DE AGUIAR; LIMA, ESTELA DE OLIVEIRA; DE OLIVEIRA, DIOGO NOIN; ODIR RODRIGUES MELO, CARLOS FERNANDO; DELAFIORI, JEANY; SOUZA GOMEZ, CARLA CRISTINA; RIBEIRO, JOSE DIRCEU; RIBEIRO, ANTONIO FERNANDO; LEVY, CARLOS EMILIO; et al. Skin Biomarkers for Cystic Fibrosis: A Potential Non-Invasive Approach for Patient Screening. FRONTIERS IN PEDIATRICS, v. 5, JAN 10 2018. (15/06809-1, 14/00302-0, 11/50400-0, 16/17066-2)

LIMA, ESTELA DE O.; GUERREIRO, TATIANE M.; MELO, CARLOS FERNANDO O. R.; DE OLIVEIRA, DIOGO N.; MACHADO, DAISY; LANCELLOTI, MARCELO; CATHARINO, RODRIGO R.. MALDI imaging detects endogenous digoxin in glioblastoma cells infected by Zika virusWould it be the oncolytic key?. Journal of Mass Spectrometry, v. 53, n. 3, p. 257-263, MAR 2018. (15/06809-1, 14/00302-0, 11/50400-0, 16/17066-2)

ODIR RODRIGUES MELO, CARLOS FERNANDO; ESTEVES, CIBELE ZANARDI; DE OLIVEIRA, ROSIMEIRE NUNES; GUERREIRO, TATIANE MELINA; DE OLIVEIRA, DIOGO NOIN; LIMA, ESTELA DE OLIVEIRA; MINE, JULIO CESAR; ALLEGRETTI, SILMARA MARQUES; CATHARINO, RODRIGO RAMOS. Early developmental stages of Ascaris lumbricoides featured by high-resolution mass spectrometry. Parasitology Research, v. 115, n. 11, p. 8-pg., 2016-11-01. (14/00084-2, 14/00302-0, 11/50400-0, 15/06809-1)

ODIR RODRIGUES MELO, CARLOS FERNANDO; ESTEVES, CIBELE ZANARDI; DE OLIVEIRA, ROSIMEIRE NUNES; GUERREIRO, TATIANE MELINA; DE OLIVEIRA, DIOGO NOIN; LIMA, ESTELA DE OLIVEIRA; MINE, JULIO CESAR; ALLEGRETTI, SILMARA MARQUES; CATHARINO, RODRIGO RAMOS. Early developmental stages of Ascaris lumbricoides featured by high-resolution mass spectrometry. Parasitology Research, v. 115, n. 11, p. 4107-4114, NOV 2016. (14/00084-2, 15/06809-1, 14/00302-0, 11/50400-0)

ODIR RODRIGUES MELO, CARLOS FERNANDO; NAVARRO, LUIZ CLAUDIO; DE OLIVEIRA, DIOGO NOIN; GUERREIRO, TATIANE MELINA; LIMA, ESTELA DE OLIVEIRA; DELAFIORI, JEANY; DABAJA, MOHAMED ZIAD; RIBEIRO, MARTA DA SILVA; DE MENEZES, MAICO; MARTINS RODRIGUES, RAFAEL GUSTAVO; et al. A Machine Learning Application Based in Random Forest for Integrating Mass Spectrometry-Based Metabolomic Data: A Simple Screening Method for Patients With Zika Virus. FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, v. 6, APR 11 2018. (14/00302-0, 11/50400-0, 15/06809-1, 16/17066-2, 17/12646-3)

FERREIRA, MONICA SIQUEIRA; DE OLIVEIRA, ROSIMEIRE NUNES; DE OLIVEIRA, DIOGO NOIN; ESTEVES, CIBELE ZANARDI; ALLEGRETTI, SILMARA MARQUES; CATHARINO, RODRIGO RAMOS. Revealing praziquantel molecular targets using mass spectrometry imaging: an expeditious approach applied to Schistosoma mansoni. International Journal for Parasitology, v. 45, n. 6, p. 385-391, MAY 2015. (14/00302-0, 11/50400-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

ESTEVES, Cibele Zanardi. Applications of metabolomics in neonatal cholestasis and cystic fibrosis: the search for markers for diagnosis using mass spectrometry. 2017. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Faculdade de Ciências Médicas Campinas, SP.