Tissue localization of tannins and Anthraquinones acting on gastrointestinal system

• Grant number: 14/18052-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: January 01, 2015
• End date: November 30, 2017
• Field of knowledge: Health Sciences - Pharmacy - Pharmacognosy
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Norberto Peporine Lopes
• Grantee: Daniel Pecoraro Demarque
• Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Several plants acting in the gastrointestinal tract - rich in tannins and anthraquinones - still have many doubts about its mechanism of action and toxicity. Plants rich in anthraquinones are widely used as laxatives and prolonged use leads to colon melanosis, while the tannins do not have a full comprehensive mechanism of action, and its action is related with their chemical properties. This project aims to administration of Cascara Sagrada and Barbatimão extract - rich in anthraquinones and tannins, respectively - in animals and identify the presence of these substances and their metabolites in gastric, intestinal and liver tissue by Imaging Mass Spectrometry (IMS). Thus, the research aims to determine the distribution of these substances and their metabolites in tissues of the gastrointestinal system leading to a better understanding of the mechanism of action and toxicity of plants that acting in this system, helping to obtain safe and effective phytomedicines. (AU)

Scientific publications

(8)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DEMARQUE, DANIEL P.; CALLEJON, DANIEL R.; DE OLIVEIRA, GIBSON G.; SILVA, DENISE B.; CAROLLO, CARLOS A.; LOPES, NORBERTO P.. The role of tannins as antiulcer agents: a fluorescence-imaging based study. REVISTA BRASILEIRA DE FARMACOGNOSIA-BRAZILIAN JOURNAL OF PHARMACOGNOSY, v. 28, n. 4, p. 425-432, JUL-AUG 2018. (14/50265-3, 14/18052-0)

DEMARQUE, DANIEL P.; DA SILVA, RUI M. P.; SANTOS, LEANDRO F.; LEOPOLDINO, ANDREIA M.; ESPREAFICO, ENILZA M.; LOPES, NORBERTO P.. Cytotoxicity of Structurally Diverse Anthranoids and Correlation with Mechanism of Action and Side Effects. JOURNAL OF PHARMACY AND PHARMACEUTICAL SCIENCES, v. 21, p. 347-353, 2018. (14/50265-3, 14/18052-0)

DEMARQUE, DANIEL P.; PINHO, DANIELLE R.; LOPES, NORBERTO P.; MERTEN, CHRISTIAN. Revisiting empirical rules for the determination of the absolute configuration of cascarosides and other (ox-)anthrones. CHIRALITY, v. 30, n. 4, p. 432-438, APR 2018. (14/18052-0)

DEMARQUE, DANIEL P.; CALLEJON, DANIEL R.; PINTO, LARISSA G.; GOUVEA, DAYANA R.; DE MORAES, NATALIA V.; LOPES, JOAO L. C.; LOPES, JOSE N. C.; LOPES, NORBERTO P.; GUARATINI, THAIS. Pharmacokinetic disposition of erythraline in rats after intravenous administration. REVISTA BRASILEIRA DE FARMACOGNOSIA-BRAZILIAN JOURNAL OF PHARMACOGNOSY, v. 29, n. 6, p. 773-777, NOV-DEC 2019. (14/50265-3, 14/18052-0)

LANCHOTI FIORI, GIOVANA MARIA; DEMARQUE, DANIEL PECORARO; SOARES PEREIRA, ANA MARIA; KLEIN, VERA L. G.; LOPES, NORBERTO PEPORINE. Cucurbitane Triterpene Glycosides from the Roots of Wilbrandia hibiscoides. REVISTA BRASILEIRA DE FARMACOGNOSIA-BRAZILIAN JOURNAL OF PHARMACOGNOSY, v. 31, n. 5, AUG 2021. (12/14408-9, 14/50265-3, 14/18052-0)

DEMARQUE, DANIEL P.; MERTEN, CHRISTIAN. Intra- versus Intermolecular Hydrogen Bonding: Solvent-Dependent Conformational Preferences of a Common Supramolecular Binding Motif from (HNMR)-H-1 and Vibrational Circular Dichroism Spectra. CHEMISTRY-A EUROPEAN JOURNAL, v. 23, n. 71, p. 17915-17922, DEC 19 2017. (14/18052-0)

DEMARQUE, DANIEL P.; CROTTI, ANTONIO E. M.; VESSECCHI, RICARDO; LOPES, JOAO L. C.; LOPES, NORBERTO P.. Fragmentation reactions using electrospray ionization mass spectrometry: an important tool for the structural elucidation and characterization of synthetic and natural products. NATURAL PRODUCT REPORTS, v. 33, n. 3, p. 432-455, 2016. (13/20094-0, 14/23604-1, 14/20302-4, 14/18052-0, 14/50265-3)

WANG, MINGXUN; CARVER, JEREMY J.; PHELAN, VANESSA V.; SANCHEZ, LAURA M.; GARG, NEHA; PENG, YAO; NGUYEN, DON DUY; WATROUS, JERAMIE; KAPONO, CLIFFORD A.; LUZZATTO-KNAAN, TAL; et al. Sharing and community curation of mass spectrometry data with Global Natural Products Social Molecular Networking. NATURE BIOTECHNOLOGY, v. 34, n. 8, p. 828-837, AUG 2016. (14/18052-0, 12/18031-7, 14/01651-8, 13/16496-5, 14/01884-2, 14/50265-3)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

DEMARQUE, Daniel Pecoraro. Chemical and biological studies of tannins and anthraquinones acting on the gastrointestinal tract. 2017. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Ciências Farmacêuticas de Ribeirão Preto (PCARP/BC) Ribeirão Preto.