Due to the high prevalence of dental erosion found in recent years, new therapies have been studied to prevent or inhibit this condition. The aim of this study is to evaluate the in situ effect of dendê oil associated or not with fluoride to prevent erosive tooth wear. This is a single cross-blind (to researcher) in situ study involving a randomized block design ( by volunteer) and it will be carried out in four phases (one phase per group) of 5 days, in which the volunteers will use palatine intraoral appliances containing 2 enamel blocks per group. Approximately 150 bovine teeth will be cutted into 4x4mm enamel blocks, grounded and polished to allow the surface hardness and the initial profilometry for selection of the blocks. Then, 96 enamel blocks will be selected and randomized between the four study groups by the initial surface hardness: GI - Dênde oil (type and concentration determined in FAPESP process number 2014 / 00102-0); GII - Dendê oil associated with fluoridated solution (Elmex Erosion protection, GABA International AG, Grabetsmattweg, Switzerland); GIII - deionized water (negative control); GIV - fluoridated solution (Elmex Erosion Protection, GABA International AG, Grabetsmattweg, Switzerland) (positive control). During the in situ period, the volunteers will treat the enamel blocks (4 times per day for 5 days) with the test solution by administration of 5 drops for each enamel block during 30 seconds and then, reinsert the intraoral appliance into the oral cavity for 2 minutes to simulate the washing action by the human saliva. Then, the appliance should be removed from the oral cavity and immersed in 50 ml of 0.05M citric acid for 2 minutes. After each acid challenge, the intraoral appliance should be washed with tap water and reinsert into the mouth. The response variable will be the profilometry by the profilometric analysis of each specimen before and after the experiment. The graphs obtained from each species will be overlapped and enamel loss will be measured. The results will be checked for normal distribution and homogeneity. After this analysis, the results will be verified with appropriate statistical test (parametric or non-parametric), considering the factors under study. The significance level will be set at 5%.
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