Molecular interaction between chitosan and cell membrane models investigated by non-linear vibrational spectroscopy

• Grant number: 17/03503-4
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: August 01, 2017
• End date: March 11, 2020
• Field of knowledge: Physical Sciences and Mathematics - Physics - Condensed Matter Physics
• Principal Investigator: Paulo Barbeitas Miranda
• Grantee: Rafael de Oliveira Pedro
• Host Institution: Instituto de Física de São Carlos (IFSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
• Associated research grant: 13/14262-7 - Nanostructured films from biologically-relevant materials, AP.TEM

Abstract

Chitosan has been used in many biological applications such as drug delivery, gene therapy, tissue engineering and antimicrobial systems. Although these applications have grown greatly in recent years, little is known about the modes of interaction at the molecular level of chitosan with biological membranes. Therefore, interactions between chitosan and membrane models of mammals, bacteria and fungi will be investigated in this work. Firstly, biologically relevant temperature will be implemented in the experiments in order to allow the extrapolation of the results obtained for in vitro or in vivo situations. Following, Sum-Frequency Generation spectroscopy (SFG) will provide information about the interaction of chitosan with the polar group of lipids, conformation of the lipid chains and the penetration of chitosan in the membrane models. Sum-Frequency Generation microscopy (MSFG microscopy), an innovative technique, will be used to obtain images at interfaces with chemical sensitivity by the vibrational spectrum and contrast by the orientation and ordering of the molecules. The techniques of polarization-modulated infrared spectroscopy (PM-IRRAS) and Brewster angle microscopy (BAM) will be used for comparison with results from SFG spectroscopy and microscopy. The results are expected to provide a solid basis for understanding the role of positive charges, degree of hydrophobicity, and hydrogen bonds in the interactions between chitosan and membrane models. Therefore, this work will contribute to elucidate the mechanism of molecular interaction of chitosan derivatives with cellular membranes of mammals, bacteria and fungi, using powerful techniques, although not widely used in this area.

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CARR, OLIVIA; RAYMUNDO-PEREIRA, PAULO A.; SHIMIZU, FLAVIO M.; SORROCHE, BRUNA PEREIRA; MELENDEZ, MATIAS ELISEO; PEDRO, RAFAEL DE OLIVEIRA; MIRANDA, PAULO B.; CARVALHO, ANDRE LOPES; REIS, RUI M.; ARANTES, LIDIA M. R. B.; et al. Genosensor made with a self-assembled monolayer matrix to detect MGMT gene methylation in head and neck cancer cell lines. Talanta, v. 210, APR 1 2020. (17/03503-4, 12/15543-7, 18/02819-0, 15/12990-0, 13/14262-7, 19/13390-8, 16/01919-6)

RAYMUNDO-PEREIRA, PAULO A.; PEDRO, RAFAEL DE OLIVEIRA; CARR, OLIVIA; MELENDEZ, MATIAS E.; GOBBI, ANGELO L.; PIAZZETTA, MARIA HELENA DE OLIVEIRA; CARVALHO, ANDRE L.; REIS, RUI M.; MIRANDA, PAULO B.; OLIVEIRA JR, OSVALDO N.. Influence of the Molecular Orientation and Ionization of Self-Assembled Monolayers in Biosensors: Application to Genosensors of Prostate Cancer Antigen 3. Journal of Physical Chemistry C, v. 125, n. 1, p. 498-506, JAN 14 2021. (16/01919-6, 17/03503-4, 18/22214-6, 18/02819-0)

PEDRO, RAFAEL DE OLIVEIRA; PEREIRA, ANDRESSA RIBEIRO; OLIVEIRA, OSVALDO N.; MIRANDA, PAULO BARBEITAS. Interaction of chitosan derivatives with cell membrane models in a biologically relevant medium. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 192, AUG 2020. (18/00878-0, 18/02819-0, 17/03503-4)

PEREIRA, ANDRESSA R.; FIAMINGO, ANDERSON; PEDRO, RAFAEL DE O.; CAMPANA-FILHO, SERGIO P.; MIRANDA, PAULO B.; OLIVEIRA JR, OSVALDO N.. Enhanced chitosan effects on cell membrane models made with lipid raft monolayers. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 193, SEP 2020. (17/03503-4, 13/14262-7, 18/00878-0, 16/09720-4)

RIMOLI, CAIO VAZ; PEDRO, RAFAEL DE OLIVEIRA; MIRANDA, PAULO B.. Interaction mechanism of chitosan oligomers in pure water with cell membrane models studied by SFG vibrational spectroscopy. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 219, p. 12-pg., 2022-09-02. (18/02819-0, 13/23100-0, 14/01595-0, 17/03503-4)

CARR, OLIVIA; RAYMUNDO-PEREIRA, PAULO A.; SHIMIZU, FLAVIO M.; SORROCHE, BRUNA PEREIRA; MELENDEZ, MATIAS ELISEO; PEDRO, RAFAEL DE OLIVEIRA; MIRANDA, PAULO B.; CARVALHO, ANDRE LOPES; REIS, RUI M.; ARANTES, LIDIA M. R. B.; et al. Genosensor made with a self-assembled monolayer matrix to detect MGMT gene methylation in head and neck cancer cell lines. Talanta, v. 210, p. 7-pg., 2020-04-01. (15/12990-0, 12/15543-7, 18/02819-0, 13/14262-7, 16/01919-6, 19/13390-8, 17/03503-4)