Langmuir and Langmuir-Blodgett films of lipids containing phenylalanine dehydrogenase for phenylalanine biosensors

• Grant number: 19/01797-6
• Support Opportunities: Scholarships abroad - Research Internship - Master's degree
• Start date: May 06, 2019
• End date: October 05, 2019
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Physical-Chemistry
• Principal Investigator: Luciano Caseli
• Grantee: Rafael Leonardo Cruz Gomes da Silva
• Supervisor: Roger M. Leblanc
• Host Institution: Instituto de Ciências Ambientais, Químicas e Farmacêuticas (ICAQF). Universidade Federal de São Paulo (UNIFESP). Campus Diadema. Diadema , SP, Brazil
• Institution abroad: University of Miami, United States
• Associated to the scholarship: 18/00291-9 - Langmuir and Langmuir-Blodgett films of lipids containing phenylalanine dehydrogenase for sensors of phenylalanine, BP.MS

Abstract

In this project, we intend to study the immobilization of the phenylalanine dehydrogenase enzyme into Langmuir and Langmuir-Blodgett films of lipids, as well as to investigate the physical-chemical properties of the produced systems and possible applications for phenylalanine detection. Beyond the classical characterization techniques applied for nanostructured films (tensiometry, surface potential measurements, rheological analysis, and Brewster angle microscopy) we propose the use of advanced spectroscopic and optical measurements (epifluorescence microscopy, Circular dichroism, Ellipsometry and in situ Uv-visible spectroscopy) to enhance our knowledge of the interactions happening at molecular level between phenylalanine dehydrogenase and lipid matrices. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CRUZ GOMES DA SILVA, RAFAEL LEONARDO; SHARMA, SHIV K.; PAUDYAL, SURAJ; MINTZ, KEENAN J.; CASELI, LUCIANO; LEBLANC, ROGER M.. Surface chemistry and spectroscopic studies of the native phenylalanine dehydrogenase Langmuir monolayer at the air/aqueous NaCl interface. Journal of Colloid and Interface Science, v. 560, p. 458-466, FEB 15 2020. (19/01797-6)

CRUZ GOMES DA SILVA, RAFAEL LEONARDO; SHARMA, SHIV K.; PAUDYAL, SURAJ; MINTZ, KEENAN J.; LEBLANC, ROGER M.; CASELI, LUCIANO. Surface Chemistry Studies on the Formation of Mixed Stearic Acid/Phenylalanine Dehydrogenase Langmuir and Langmuir-Blodgett Films. Langmuir, v. 37, n. 25, p. 7771-7779, JUN 29 2021. (19/01797-6, 18/22214-6, 18/00291-9, 19/03239-0)