GLUT4 translocation is not impaired after acute exercise in skeletal muscle of women with obesity and polycystic ovary syndrome

Author(s):Show less -

Dantas, Wagner Silva ^[1] ; Miguel Marcondes, Jose Antonio ^[2] ; Shinjo, Samuel Katsuyuki ^[3] ; Perandini, Luiz Augusto ^[3] ; Zambelli, Vanessa Olzon ; Das Neves, Willian ^[1] ; Grimaldi Barcellos, Cristiano Roberto ^[2] ; Rocha, Michele Patrocinio ^[2] ; Vieira Yance, Viviane Dos Reis ^[2] ; Dos Santos Pereira, Renato Tavares ^[1] ; Murai, Igor Hisashi ^[1] ; De Sa Pinto, Ana Lucia ^[3] ; Roschel, Hamilton ^{[3, 1]} ; Gualano, Bruno ^{[3, 1]}

Affiliation

^[1] Univ Sao Paulo, Sch Phys Educ & Sport, Dept Biodynam Human Movement, BR-05508 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Sch Med, Div Endocrinol, BR-05508 Sao Paulo - Brazil

^[3] Univ Sao Paulo, Sch Med, Div Rheumatol, BR-05508 Sao Paulo - Brazil

• Document type: Journal article
• Source: OBESITY; v. 23, n. 11, p. 2207-2215, NOV 2015.
• Web of Science Citations: 7

Abstract

ObjectiveThe aim of this study was to examine the effects of acute exercise on insulin signaling in skeletal muscle of women with polycystic ovary syndrome (PCOS) and controls (CTRL). MethodsFifteen women with obesity and PCOS and 12 body mass index-matched CTRL participated in this study. Subjects performed a 40-min single bout of exercise. Muscle biopsies were performed before and 60 min after exercise. Selected proteins were assessed by Western blotting. ResultsCTRL, but not PCOS, showed a significant increase in PI3-k p85 and AS160 Thr 642 after a single bout of exercise (P=0.018 and P=0.018, respectively). Only PCOS showed an increase in Akt Thr 308 and AMPK phosphorylation after exercise (P=0.018 and P=0.018, respectively). Total GLUT4 expression was comparable between groups (P>0.05). GLUT4 translocation tended to be significantly higher in both groups after exercise (PCOS: P=0.093; CTRL: P=0.091), with no significant difference between them (P>0.05). ConclusionsA single bout of exercise elicited similar GLUT4 translocation in skeletal muscle of PCOS and CTRL, despite a slightly differential pattern of protein phosphorylation. The absence of impairment in GLUT4 translocation suggests that PCOS patients with obesity and insulin resistance may benefit from exercise training. (AU)

• FAPESP's process: 12/14650-4 - Acute effect of exercise on molecular pathways related to skeletal muscle glucose uptake in patients with polycystic ovary syndrome
• Grantee: Bruno Gualano
• Support Opportunities: Regular Research Grants
• FAPESP's process: 12/02827-7 - Acute effects of exercise training on molecular pathways of glucose uptake in skeletal muscle in patients with Polycystic Ovary Syndrome
• Grantee: Wagner Silva Dantas
• Support Opportunities: Scholarships in Brazil - Master