High loading of graphene oxide/multi-walled carbon nanotubes into PDLLA: A route towards the design of osteoconductive, bactericidal and non-immunogenic 3D porous scaffolds

Author(s)

Zanin, Hudson ^{[1, 2]} ; Manzolli Rodrigues, Bruno Vinicius ^[1] ; Ribeiro Neto, Wilson Alves ^[3] ; Suman Bretas, Rosario Elida ^[3] ; Da-Silva, Newton Soares ^[4] ; Marciano, Fernanda Roberta ^[1] ; Lobo, Anderson Oliveira ^[1]

Affiliation

^[1] Univ Vale Paraiba, Inst Res & Dev, Lab Biomed Nanotechnol Nanobio, Av Shishima Hifumi 2911, BR-12244000 Sao Jose Dos Campos, SP - Brazil

^[2] Univ Vale Paraiba, Inst Res & Dev, Lab Energy Storage & Supply ES&S, Av Shishima Hifumi 2911, BR-12244000 Sao Jose Dos Campos, SP - Brazil

^[3] Univ Fed Sao Carlos, Dept Mat Engn, Rodovia Washington Luis, Km 235, SP-310, Sao Carlos, SP - Brazil

^[4] Univ Vale Paraiba, Lab Cell Biol & Tissue, Inst Res & Dev, Av Shishima Hifumi 2911, BR-12244000 Sao Jose Dos Campos, SP - Brazil

• Document type: Journal article
• Source: Materials Chemistry and Physics; v. 177, p. 56-66, JUL 1 2016.
• Web of Science Citations: 4

Abstract

We have prepared a novel 3D porous biomaterial combining poly (DL-lactic acid) (PDLLA) and graphene and multi-walled carbon nanotubes oxides (MWCNTO-GO) composite. PDLLA as control and a high loading of PDLLA/MWCNTO-GO (50/50 w/w) bioscaffolds were prepared and functionalized. MWCNTs were exfoliated to form MWCNTO-GO by oxygen plasma etching. The later was also applied to enhance the scaffolds wettability, attaching oxygen-containing groups on their surfaces. This approach produced a porous architecture observed by scanning electron microscopy and semi-quantified by electrochemical analysis. The later also indicated a notable increase on the conductivity of PDLLA/MWCNTO-GO scaffold compared to MWCNTO-GO free PDLLA (about 5 orders of magnitudes at low frequencies). Thermogravimetric analysis showed that the MWCNTO-GO acted protecting the PDLLA matrix, enhancing its thermal stability. The PDLLA/MWCNTO-GO scaffolds had significant cellular adhesion, did not present cytotoxicity effect, besides reduced bactericidal proliferation and produced mineralized tissues in SBF media. The metallic MWCNTO-GO powder held together by PDLLA polymer opens a whole new branch of applications, including bioelectroanalyses, drug delivery systems and tissue engineering. (C) 2016 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 11/20345-7 - Study of nanoparticle-incorporated diamond-like carbon films for biomedical applications
• Grantee: Fernanda Roberta Marciano
• Support type: Research Grants - Young Investigators Grants
• FAPESP's process: 15/08523-8 - Development of a fluorescent nanosensor from the electrospinning of PVA and graphene quantum dots: application in the detection of Alzheimer's biomarkers
• Grantee: Bruno Vinícius Manzolli Rodrigues
• Support type: Scholarships in Brazil - Post-Doctorate
• FAPESP's process: 11/17877-7 - Development of new polymeric scaffolds by electrospinning technique with incorporation of vertically aligned carbon nanotubes and nanohidroxyapatite for bone tissue regeneration
• Grantee: Anderson de Oliveira Lobo
• Support type: Research Grants - Young Investigators Grants
• FAPESP's process: 15/01259-3 - Electrospinning by solution of PBAT built nanohidroxiapatita
• Grantee: Aline dos Santos Silva
• Support type: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 14/02163-7 - Development of supercapacitors devices from graphene, carbon nanotubes and diamonds
• Grantee: Hudson Giovani Zanin
• Support type: Research Grants - Young Investigators Grants
• FAPESP's process: 13/20054-8 - Studies of adhesion, proliferation and cell death in the interaction of cells with nanostructured materials
• Grantee: Newton Soares da Silva
• Support type: Regular Research Grants