Network of proteins, enzymes and genes linked to biomass degradation shared by Trichoderma species

Author(s)

Crivelente Horta, Maria Augusta ^[1] ; Ferreira Filho, Jaire Alves ^[1] ; Murad, Natalia Faraj ^[1] ; Santos, Eidy de Oliveira ^[2] ; dos Santos, Clelton Aparecido ^[1] ; Mendes, Juliano Sales ^[1] ; Brandao, Marcelo Mendes ^[1] ; Azzoni, Sindelia Freitas ^[3] ; de Souza, Anete Pereira ^{[1, 4]}

Affiliation

^[1] Univ Campinas UNICAMP, Ctr Mol Biol & Genet Engn CBMEG, Campinas, SP - Brazil

^[2] West Zone State Univ UEZO, Univ Unit Biol, Rio De Janeiro, RJ - Brazil

^[3] Brazilian Ctr Res Energy & Mat CNPEM, Bioethanol Sci & Technol Lab CTBE, Campinas, SP - Brazil

^[4] Univ Campinas UNICAMP, Biol Inst, Dept Plant Biol, Campinas, SP - Brazil

• Document type: Journal article
• Source: SCIENTIFIC REPORTS; v. 8, JAN 22 2018.
• Web of Science Citations: 4

Abstract

Understanding relationships between genes responsible for enzymatic hydrolysis of cellulose and synergistic reactions is fundamental for improving biomass biodegradation technologies. To reveal synergistic reactions, the transcriptome, exoproteome, and enzymatic activities of extracts from Trichoderma harzianum, Trichoderma reesei and Trichoderma atroviride under biodegradation conditions were examined. This work revealed co-regulatory networks across carbohydrate-active enzyme (CAZy) genes and secreted proteins in extracts. A set of 80 proteins and respective genes that might correspond to a common system for biodegradation from the studied species were evaluated to elucidate new co-regulated genes. Differences such as one unique base pair between fungal genomes might influence enzyme-substrate binding sites and alter fungal gene expression responses, explaining the enzymatic activities specific to each species observed in the corresponding extracts. These differences are also responsible for the different architectures observed in the co-expression networks. (AU)

• FAPESP's process: 16/19775-0 - Development and/or improvement of enzymatic cocktails designed for plant biomass hydrolysis: use of site-directed mutagenesis and chimeric proteins design
• Grantee: Clelton Aparecido dos Santos
• Support type: Scholarships in Brazil - Post-Doctorate
• FAPESP's process: 15/09202-0 - Study of Trichoderma harzianum genomic regions associated to the control of the expression of the enzymes involved in the degradation of biomass
• Grantee: Anete Pereira de Souza
• Support type: Regular Research Grants
• FAPESP's process: 15/50612-8 - An integrated approach to explore a novel paradigm for biofuel production from lignocellulosic feedstocks
• Grantee: Telma Teixeira Franco
• Support type: Research Projects - Thematic Grants
• FAPESP's process: 11/00417-3 - System biology techniques applied to the agriculture: transcriptomes and interactomes analyses
• Grantee: Marcelo Mendes Brandao
• Support type: Research Grants - Young Investigators Grants
• FAPESP's process: 14/18856-1 - Study of global gene expression and genomic regions associated with control expression of enzymes involved in the degradation of lignocellulosic compounds by Trichoderma harzianum
• Grantee: Maria Augusta Crivelente Horta
• Support type: Scholarships in Brazil - Post-Doctorate