Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

15d-PGJ(2)-loaded nanocapsules ameliorate experimental gout arthritis by reducing pain and inflammation in a PPAR-gamma-sensitive manner in mice

Full text
Author(s):
Show less -
Ruiz-Miyazawa, Kenji W. [1] ; Staurengo-Ferrari, Larissa [1] ; Pinho-Ribeiro, Felipe A. [1] ; Fattori, Victor [1] ; Zaninelli, Tiago H. [1] ; Badaro-Garcia, Stephanie [1] ; Borghi, Sergio M. [1] ; Andrade, Ketlem C. [1] ; Clemente-Napimoga, Juliana T. [2] ; Alves-Filho, Jose C. [3] ; Cunha, Thiago M. [3] ; Fraceto, Leonardo F. [4] ; Cunha, Fernando Q. [3] ; Napimoga, Marcelo H. [2] ; Casagrande, Rubia [5] ; Verri, Jr., Waldiceu A. [1]
Total Authors: 16
Affiliation:
[1] Univ Estadual Londrina, Dept Ciencias Patol, Rod Celso Garcia Cid, Km 380, PR445, BR-86057970 Londrina, Parana - Brazil
[2] Sao Leopoldo Mand Inst & Researcher Ctr, Lab Immunol & Mol Biol, Campinas, SP - Brazil
[3] Univ Sao Paulo, Dept Pharmacol, Ribeirao Preto Med Sch, Ave Bandeirantes S-N, BR-14050490 Sao Paulo - Brazil
[4] Sao Paulo State Univ, Dept Environm Engn, Sorocaba - Brazil
[5] Univ Estadual Londrina, Dept Ciencias Farmaceut, Univ Hosp, Ave Robert Koch 60, BR-86038350 Londrina, Parana - Brazil
Total Affiliations: 5
Document type: Journal article
Source: SCIENTIFIC REPORTS; v. 8, SEP 18 2018.
Web of Science Citations: 8
Abstract

Gout arthritis (GA) is a painful inflammatory disease in response to monosodium urate (MSU) crystals in the joints. 15deoxy-Delta(12,14)-prostaglandin J(2) (15d-PGJ(2)) is a natural activator of PPAR-gamma with analgesic, anti-inflammatory, and pro-resolution properties. Thus, we aimed to evaluate the effect and mechanisms of action of 15d-PGJ(2) nanocapsules (NC) in the model of GA in mice, since a reduction of 33-fold in the dose of 15d-PGJ2 has been reported. Mice were treated with 15d-PGJ(2)-loaded NC, inert NC, free 15d-PGJ(2) (without NC), or 15d-PGJ(2)-loaded NC+GW9662, a PPAR-gamma inhibitor. We show that 15d-PGJ(2)-loaded NC provided analgesic effect in a dose that the free 15d-PGJ(2) failed to inhibiting pain and inflammation. Hence, 15d-PGJ(2)-loaded NC reduced MSU-induced IL-1 beta, TNF-alpha, IL-6, IL-17, and IL-33 release and oxidative stress. Also, 15d-PGJ(2)-loaded NC decreased the maturation of IL-1 beta in LPS-primed BMDM triggered by MSU. Further, 15d-PGJ(2)-loaded NC decreased the expression of the components of the inflammasome Nlrp3, Asc, and Pro-caspase-1, as consequence of inhibiting NF-kappa B activation. All effects were PPAR-gamma-sensitive. Therefore, we demonstrated that 15d-PGJ(2)-loaded NC present analgesic and anti-inflammatory properties in a PPAR-gamma-dependent manner inhibiting IL-1 beta release and NF-kappa B activation in GA. Concluding, 15d-PGJ(2)-loaded NC ameliorates MSU-induced GA in a PPAR-gamma-sensitive manner. (AU)

FAPESP's process: 13/08216-2 - CRID - Center for Research in Inflammatory Diseases
Grantee:Fernando de Queiroz Cunha
Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
FAPESP's process: 10/15014-9 - Evaluation of the effect of 15d-PGJ2-loaded on polymeric biodegradable nanoparticles to control the evolution of the experimental periodontal disease
Grantee:Marcelo Henrique Napimoga
Support Opportunities: Regular Research Grants
FAPESP's process: 11/19670-0 - Mechanisms involved in the pathophysiology of rheumatoid arthritis, pain and sepsis
Grantee:Fernando de Queiroz Cunha
Support Opportunities: Research Projects - Thematic Grants