Encapsulation of anthocyanin-rich extract from blackberry residues by spray-drying, freeze-drying and supercritical antisolvent

Author(s)

da Fonseca Machado, Ana Paula ^[1] ; Rezende, Camila Alves ^[2] ; Rodrigues, Rodney Alexandre ^[3] ; Fernandez Barbero, Gerardo ^[4] ; Vieira e Rosa, Paulo de Tarso ^[2] ; Martinez, Julian ^[1]

Affiliation

^[1] Univ Estadual Campinas, Coll Food Engn, Dept Food Engn, BR-13083862 Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Inst Chem, BR-13083861 Campinas, SP - Brazil

^[3] Univ Estadual Campinas, Chem Biol & Agr Pluridisciplinar Res Ctr CPQBA, BR-13148218 Paulinia, SP - Brazil

^[4] Univ Cadiz, Fac Sci, Dept Analyt Chem, IVAGRO, Republ Saharaui Ave, Cadiz - Spain

• Document type: Journal article
• Source: Powder Technology; v. 340, p. 553-562, DEC 2018.
• Web of Science Citations: 5

Abstract

The extract obtained from blackberry (Rubus fruticosus) residues was encapsulated in polyvinylpyrrolidone (PVP) by three methods: the conventional spray-drying (SD) and freeze-drying (FD) techniques, and the new method of supercritical antisolvent (SAS), using CO2 as antisolvent and ethanol as solvent of the organic solution (extract + PVP). The methods and their produced particles were evaluated in terms of precipitation yield, residual ethanol and moisture contents, anthocyanin concentration, antioxidant capacity, morphology, crystallinity and thermal stability. SD,FD and SAS achieved particles with good anthocyanin yields (above 76%), high antioxidant capacity (above 1001.unol TE/g particle) and were effective to concentrate anthocyanins in PVP without great degradation. Using SAS, particles with 1.42 mg ECy3G1/g were achieved. Nevertheless, SAS particles presented high residual ethanol (8.17% w/w) and moisture (1130% w/w), whereas in SD and FD particles these contents remained below 2 and 5%, respectively. Scanning electron microscopy revealed a spherical shape in the particles obtained by SD, while those produced by SAS and FD presented irregular agglomerates. The encapsulation processes were equivalent in terms of thermal protection of the extracts and they did not modify the crystallinity and thermal behavior of PVP. The SAS process achieved preferential precipitation of anthocyanins when compared to SD and FD, since supercritical CO2 does not have any affinity to such compounds. (C) 2018 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 16/13602-7 - Study on strategies to allow the integral use of lignocellulosic biomass
• Grantee: Camila Alves de Rezende
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Regular Program Grants
• FAPESP's process: 15/11932-7 - Integration of high pressure extraction to separation operations for the recovery of by-products from fruit processing
• Grantee: Julian Martínez
• Support Opportunities: Regular Research Grants
• FAPESP's process: 13/02203-6 - Development of equipments for extraction, particle formation and chemical reactions using pressurized fluids
• Grantee: Julian Martínez
• Support Opportunities: Regular Research Grants