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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Persistence and Intra-Host Genetic Evolution of Zika Virus Infection in Symptomatic Adults: A Special View in the Male Reproductive System

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Oliveira, Danielle B. L. [1] ; Durigon, Giuliana S. [2] ; Mendes, Erica A. [1] ; Ladner, Jason T. [3, 4] ; Andreata-Santos, Robert [1] ; Araujo, Danielle B. [1] ; Botosso, Viviane F. [5] ; Paola, Nicholas D. [1] ; Neto, Daniel F. L. [1] ; Cunha, Marielton P. [1] ; Braconi, Carla T. [1] ; Alves, Rubens P. S. [1] ; Jesus, Monica R. [1] ; Pereira, Lennon R. [1] ; Melo, Stella R. [1] ; Mesquita, Flavio S. [1] ; Silveira, Vanessa B. [1] ; Thomazelli, Luciano M. [1] ; Favoretto, Silvana R. [6] ; Almonfrey, Franciane B. [2] ; Abdulkader, Regina C. R. M. [2] ; Gabrili, Joel M. [7, 5] ; Tambourgi, Denise V. [5] ; Oliveira, Sergio F. [8] ; Prieto, Karla [3, 9] ; Wiley, Michael R. [3, 9] ; Ferreira, Luis C. S. [1] ; Silva, Marcos V. [10, 11] ; Palacios, Gustavo F. [3] ; Zanotto, Paolo M. A. [1] ; Durigon, Edison L. [1]
Total Authors: 31
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[1] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, BR-05508000 Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Med Sch Clin Hosp, BR-05403000 Sao Paulo, SP - Brazil
[3] US Army, Med Res Inst Infect Dis, Ctr Genome Sci, Frederick, MD 21702 - USA
[4] No Arizona Univ, Pathogen & Microbiome Inst, Flagstaff, AZ 86011 - USA
[5] Butantan Inst, Virol Lab, BR-05503900 Sao Paulo, SP - Brazil
[6] Pasteur Inst, State Hlth Dept, BR-1103000 Sao Paulo, SP - Brazil
[7] Butantan Inst, Immunochem Lab, BR-05503900 Sao Paulo, SP - Brazil
[8] Univ Sao Paulo, Inst Biomed Sci, Dept Cellular & Dev Biol, BR-05508000 Sao Paulo, SP - Brazil
[9] Univ Nebraska Med Ctr, Dept Environm Agr & Occupat Hlth, Omaha, NE 68198 - USA
[10] Inst Infectol Emilio Ribas, BR-01246900 Sao Paulo, SP - Brazil
[11] Pontificia Univ Catolica PUC SP, BR-01246900 Sao Paulo, SP - Brazil
Total Affiliations: 11
Document type: Journal article
Source: Viruses-Basel; v. 10, n. 11 NOV 2018.
Web of Science Citations: 3

We followed the presence of Zika virus (ZIKV) in four healthy adults (two men and two women), for periods ranging from 78 to 298 days post symptom onset. The patients were evaluated regarding the presence of the virus in different body fluids (blood, saliva, urine and semen), development of immune responses (including antibodies, cytokines and chemokines), and virus genetic variation within samples collected from semen and urine during the infection course. The analysis was focused primarily on the two male patients who shed the virus for up to 158 days after the initial symptoms. ZIKV particles were detected in the spermatozoa cytoplasm and flagella, in immature sperm cells and could also be isolated from semen in cell culture, confirming that the virus is able to preserve integrity and infectivity during replication in the male reproductive system (MRS). Despite the damage caused by ZIKV infection within the MRS, our data showed that ZIKV infection did not result in infertility at least in one of the male patients. This patient was able to conceive a child after the infection. We also detected alterations in the male genital cytokine milieu, which could play an important role in the replication and transmission of the virus which could considerably increase the risk of ZIKV sexual spread. In addition, full genome ZIKV sequences were obtained from several samples (mainly semen), which allowed us to monitor the evolution of the virus within a patient during the infection course. We observed genetic changes over time in consensus sequences and lower frequency intra-host single nucleotide variants (iSNV), that suggested independent compartmentalization of ZIKV populations in the reproductive and urinary systems. Altogether, the present observations confirm the risks associated with the long-term replication and shedding of ZIKV in the MRS and help to elucidate patterns of intra-host genetic evolution during long term replication of the virus. (AU)

FAPESP's process: 16/03605-9 - Temporal expression of Zika Virus genes from strains of African and Brazilian and mapping of the gene expression in permissive, semi- permissive and non- permissive systems
Grantee:Daniel Ferreira de Lima Neto
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 14/27228-4 - Protective effect of GBV-C in positive HIV patients
Grantee:Érica Araújo Mendes
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 13/26942-2 - Development of a vaccine formulation against dengue based on the nonstructural protein 1 (NS1) administered by the intradermal route
Grantee:Lennon Ramos Pereira
Support type: Scholarships in Brazil - Master
FAPESP's process: 16/08727-5 - Development of serological assay for Zika virus IgG and IgM based on low coast ELISA
Grantee:Danielle Bruna Leal de Oliveira
Support type: Research Grants - Innovative Research in Small Business - PIPE
FAPESP's process: 14/17766-9 - A systemic approach to study permissivity on the Anticarsia gemmatalis multiple nucleopolyhedrovirus (AgMNPV)
Grantee:Paolo Marinho de Andrade Zanotto
Support type: Regular Research Grants
FAPESP's process: 14/03911-7 - Evaluation of host-parasite interaction of the AgMNPV baculovirus with distinct lepidopteran cell lines
Grantee:Carla Torres Braconi
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 14/16333-1 - Rabies in terrestrial wild fauna from de Northeast region of Brazil: molecular epidemiology and detection of immune response
Grantee:Silvana Regina Favoretto
Support type: Regular Research Grants
FAPESP's process: 16/20045-7 - Antigen discovery and development of serological diagnostic methods and vaccine approaches against the Zika Virus (ZIKV)
Grantee:Luis Carlos de Souza Ferreira
Support type: Research Projects - Thematic Grants
FAPESP's process: 15/25643-7 - Molecular characterization and epidemiologic profile of human adenovirus infection in pediatric patients with acute respiratory infection in São Paulo
Grantee:Flávio da Silva Mesquita
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 13/07467-1 - CeTICS - Center of Toxins, Immune-Response and Cell Signaling
Grantee:Hugo Aguirre Armelin
Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC
FAPESP's process: 16/08204-2 - Genetic and phylodynamics diversity of emerging and re-emerging arboviruses (DENV, ZIKV and CHIKV) in the Northeast and Southeast regions of Brazil, 2014-2016
Grantee:Marielton dos Passos Cunha
Support type: Scholarships in Brazil - Doctorate