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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Label-free evaluation of small-molecule-protein interaction using magnetic capture and electrochemical detection

Full text
Author(s):
Uliana, Carolina V. [1] ; de Oliveira, Tassia R. [1] ; Cominetti, Marcia R. [2] ; Faria, Ronaldo C. [1]
Total Authors: 4
Affiliation:
[1] Univ Fed Sao Carlos, Dept Chem, BR-13565905 Sao Carlos, SP - Brazil
[2] Univ Fed Sao Carlos, Dept Gerontol, BR-13565905 Sao Carlos, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: ANALYTICAL AND BIOANALYTICAL CHEMISTRY; v. 411, n. 10, p. 2111-2119, APR 2019.
Web of Science Citations: 0
Abstract

The evaluation of interaction between small molecules and protein is an important step in the discovery of new drugs and to study complex biological systems. In this work, an alternative method was presented to evaluate small-molecule-protein interaction by using ligand capture by protein-coated magnetic particles (MPs) and disposable electrochemical cells. The interaction study was conducted using {[}10]-gingerol from ginger rhizome and a transmembrane protein alpha V beta 3 integrin. Initially, the electrochemical behavior of the natural compound {[}10]-gingerol was evaluated with the disposable carbon-based electrodes and presented an irreversible oxidation process controlled by diffusion. The analytical curve for {[}10]-gingerol was obtained in the range of 1.0 to 20.0 mu mol L-1, with limit of detection of 0.26 mu mol L-1. Then MPs coated with V3 integrin were incubated with standard solutions and extracts of ginger rhizome for {[}10]-gingerol capture and separation. The bioconjugate obtained was dropped to the disposable electrochemical cells, keeping a permanent magnet behind the working electrode, and the binding process was evaluated by the electrochemical detection of {[}10]-gingerol. The assay method proposed was also employed to calculate the {[}10]-gingerol-alpha V beta 3 integrin association constant, which was calculated as 4.3 x 10(7) M-1. The method proposed proved to be a good label-free alternative to ligand-protein interaction studies. (AU)

FAPESP's process: 15/24940-8 - EFFECTIVENESS OF STRUCTURAL CHANGES IN [10]-GINGEROL MOLECULE IN COMBINATION WITH THE CHEMOTHERAPEUTIC DOXORUBICIN FOR THE TREATMENT OF BREAST CANCER: IN VITRO AND IN VIVO STUDIES. ABSTRACT
Grantee:Márcia Regina Cominetti
Support Opportunities: Regular Research Grants
FAPESP's process: 14/22401-0 - Development of disposable immunosensors for detection of Alzheimer's Disease biomarkers
Grantee:Tássia Regina de Oliveira
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 15/19890-1 - Development of methods for diagnosis of diseases using magnetic particles and an array of imunossensors in a disposable microfluidic system
Grantee:Ronaldo Censi Faria
Support Opportunities: Regular Research Grants