Immunosensors containing solution blow spun fibers of poly(lactic acid) to detect p53 biomarker

Author(s)

Soares, Andrey Coatrini ^{[1, 2]} ; Soares, Juliana Coatrini ^{[1, 2]} ; Paschoalin, Rafaella Takehara ^[2] ; Rodrigues, Valquiria Cruz ^[2] ; Melendez, Matias Eliseo ^{[3, 4]} ; Reis, Rui M. ^{[3, 5, 6]} ; Carvalho, Andre Lopes ^[3] ; Capparelli Mattoso, Luiz Henrique ^{[1, 7]} ; Oliveira Jr, Osvaldo N.

Affiliation

^[1] Embrapa Instrumentat, Nanotechnol Natl Lab Agribusiness LNNA, BR-13560970 Sao Carlos, SP - Brazil

^[2] Univ Sao Paulo, Sao Carlos Inst Phys, BR-13566590 Sao Carlos - Brazil

^[3] Barretos Canc Hosp, Mol Oncol Res Ctr, BR-14784400 Barretos - Brazil

^[4] Little Prince Coll, Pele Little Prince Res Inst, Little Prince Complex Curitiba, BR-80250060 Curitiba, Parana - Brazil

^[5] Univ Minho, Sch Med, Life & Hlth Sci Res Inst ICVS, P-4710057 Braga - Portugal

^[6] ICVS 3Bs, PT Govt Associate Lab, P-4805017 Braga - Portugal

^[7] Univ Fed Sao Carlos, Mat Engn Dept, BR-13565905 Sao Carlos, SP - Brazil

• Document type: Journal article
• Source: Materials Science & Engineering C-Materials for Biological Applications; v. 115, OCT 2020.
• Web of Science Citations: 0

Abstract

This paper reports on biosensors made with a matrix of polylactic acid (PLA) fibers, which are suitable for immobilization of the anti-p53 active layer for detection of p53 biomarker. The PLA fibers were produced with solution blow spinning, a method that is advantageous for its simplicity and possibility to tune the fiber properties. For the biosensors, the optimized time to deposit the fibers was 60 s, with which detection of p53 could be achieved with the limit of detection of 11 pg/mL using electrical impedance spectroscopy. This sensitivity is also sufficient to detect the p53 biomarker in patient samples, which was confirmed by distinguishing samples from cell lines with distinct p53 concentrations in a plot where the impedance spectra were visualized with the interactive document mapping (IDMAP) technique. The high sensitivity and selectivity of the biosensors may be attributed to the specific interaction between the active layer and p53 modeled with a Langmuir-Freundlich and Freundlich isotherms and inferred from the analysis of the vibrational bands at 1550, 1650 and 1757 cm(-1) using polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS). The successful immobilization of the active layer is evidence that the approach based on solution blown spun fibers may be replicated to other types of biosensors. (AU)

• FAPESP's process: 18/18953-8 - Nanostructured films applied in microfluidic biosensors to mastitis detection
• Grantee: Andrey Coatrini Soares
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 13/14262-7 - Nanostructured films from biologically-relevant materials
• Grantee: Osvaldo Novais de Oliveira Junior
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 17/18725-2 - Hybrid core-shell and Janus nanofibers obtained by solution blow spinning
• Grantee: Rafaella Takehara Paschoalin
• Support Opportunities: Scholarships in Brazil - Post-Doctoral