High- and moderate-intensity training modify LPS-induced ex-vivo interleukin-10 production in obese men in response to an acute exercise bout

Author(s)

Gerosa-Neto, Jose ^{[1, 2]} ; Monteiro, Paula Alves ^[1] ; Inoue, Daniela Sayuri ^[1] ; Antunes, Barbara Moura ^[1] ; Batatinha, Helena ^[3] ; Dorneles, Gilson Pires ^[4] ; Peres, Alessandra ^[4] ; Rosa-Neto, Jose Cesar ^[3] ; Lira, Fabio Santos ^[1]

Affiliation

^[1] Univ Estadual Paulista UNESP, Dept Phys Educ, Post Grad Program Movement Sci, Exercise & Immunometab Res Grp, Presidente Prudente, SP - Brazil

^[2] Univ Ctr Maringa UniCesumar, Phys Educ, Physiotherapy, Maringa, Parana - Brazil

^[3] Univ Sao Paulo, Inst Biomed Sci, Dept Cell & Dev Biol, Sao Paulo - Brazil

^[4] Fed Univ Hlth Sci Porto Alegre UFCSPA, Dept Hlth Basic Sci, Porto Alegre, RS - Brazil

• Document type: Journal article
• Source: CYTOKINE; v. 136, DEC 2020.
• Web of Science Citations: 0

Abstract

The aim of this study was to evaluate the inflammatory (peripheral and lipopolysaccharide (LPS)-stimulated released from whole blood) and metabolic (glucose and insulin) profile of inactive obese men in response to two isoenergetic models of aerobic exercise training (similar to 300 kcal each exercise session). Twenty-two participants (28.7 +/- 1.6 years; BMI = 34.4 +/- 0.1 kg/m(2)) were randomized into two groups: I) HIIT: high-intensity interval training (10x 1 bout: 1 min - 100% Maximal Aerobic Velocity) or II) MICT: moderate-intensity continuous training (65% Maximal Aerobic Velocity; kcal equal to HIIT). Both groups trained three times per week for 6-weeks. Fasting blood samples were collected before and 0, 30, and 60 min after exercise during the first and last training sessions for evaluation of: I) MIP-1., insulin, glucose, visceral and subcutaneous fat depots, oral glucose tolerance test, and homeostatic model assessment of insulin resistance (HOMA-IR) index; II) Peripheral (TNF-alpha, IL-6, and IL-10) and LPS-stimulated release of TNF-alpha and IL-10 were analyzed before, 0, and 60 min after sessions. IL-6 concentration remained elevated up to 60-min after the acute exercise session (p < 0.001), and IL-10 concentration was higher after 30 and 60-min (p = 0.001) compared to rest, independent of training period and protocol. AUC of IL-10 presented effect of type of training (p = 0.023) with MICT group showed significantly higher values than the HIIT. The ex-vivo assay showed higher IL-10 secretion in response to LPS immediately (p = 0.003) after both acute MICT and HIIT exercise sessions, independent of training period. Fifteen subjects presented decreased HOMA-IR after 6-weeks and seven presented an increase in this index. When we excluded the two least responsive subjects, it was possible to observe a decrease in HOMA-IR (p = 0.020) after training. Taken together, our results suggest that both HIIT and MICT (with same energy expenditure) promote similar effects on HOMA-IR and led to elevations in IL-10 production in LPS-stimulated whole blood, suggesting that leukocytes had an enhanced ability to secrete anti-inflammatory cytokines after the exercise bout. (AU)

• FAPESP's process: 16/16712-8 - BDNF involvement in the function of lymphocytes of sedentary obese and trained: relationship with cognitive
• Grantee: Fábio Santos de Lira
• Support Opportunities: Regular Research Grants
• FAPESP's process: 15/22402-9 - "Impact of high intensity interval exercise on cognitive function, morphological changes and systemic imunometabólicas of young people with obesity"
• Grantee: Daniela Sayuri Inoue Yoshimura
• Support Opportunities: Scholarships in Brazil - Doctorate