Structural and viscoelastic properties of floating monolayers of a pectinolytic enzyme and their influence on the catalytic properties

Author(s)

Rodrigues, Raul T. ^[1] ; Jr., Jose R. Siqueira ; Caseli, Luciano ^[1]

Affiliation

^[1] Fed Univ Sao Paulo UNIFESP, Inst Environm Chem & Pharmaceut Sci, BR-09913030 Diadema, SP - Brazil

• Document type: Journal article
• Source: Journal of Colloid and Interface Science; v. 589, p. 568-577, MAY 2021.
• Web of Science Citations: 0

Abstract

Hypothesis: The catalytic activity of enzymes immobilized in self-assembly systems as LangmuirBlodgett (LB) films is influenced by molecular interactions dictated by the composition and viscoelasticity of the previous floating monolayers. We believe that the insertion of carbon nanotubes (CNT) in mixed polygalacturonase/lipid monolayers may influence intermolecular interactions and viscoelastic properties, being then possible to tune system stability and rheological properties, driving catalytic properties of the films for biosensing. Experiments: The physicochemical properties of the monolayers were investigated by tensiometry, surface potential, Brewster angle microscopy, infrared spectroscopy, and dilatational rheology. The monolayers were transferred to solid supports LB films and characterized by atomic force microscopy, quartz crystal microbalance, and fluorescence spectroscopy. The catalytic activity of the LB films was verified by colorimetric assay. Findings: The enzyme-CNT-lipid film had a catalytic activity at least twice as high as the pure enzyme owing to the synergy between the components, with the lipid acting as a protector matrix for the enzyme and the CNTs acting as an energy transfer facilitator. These results point to a proof-of-concept system, through which we can propose an alternative to achieve enhanced bio-inspired films with high control of the molecular architecture by using the LB approach. (c) 2021 Elsevier Inc. All rights reserved. (AU)

• FAPESP's process: 14/50869-6 - INCT 2014: on Organic Electronics
• Grantee: Roberto Mendonça Faria
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 18/22214-6 - Towards a convergence of technologies: from sensing and biosensing to information visualization and machine learning for data analysis in clinical diagnosis
• Grantee: Osvaldo Novais de Oliveira Junior
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 18/22091-1 - Langmuir and Langmuir-Blodgett films of lipid containing pectinase for pectin sensors
• Grantee: Raul Torres Rodrigues
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 19/03239-0 - Nanostructured interfaces for the investigation of bioactive substances in cell membrane models and for the construction of optoelectronic devices
• Grantee: Luciano Caseli
• Support Opportunities: Regular Research Grants