Ultrafiltration performance of spent brewer's yeast protein hydrolysate: Impact of pH and membrane material on fouling

Author(s)

Marson, Gabriela Vollet ^{[1, 2]} ; Vitor Pereira, Debora Tamires ^[3] ; da Costa Machado, Mariana Teixeira ^[4] ; Di Luccio, Marco ^[5] ; Martinez, Julian ^[3] ; Belleville, Marie-Pierre ^[2] ; Hubinger, Miriam Dupas ^[1]

Affiliation

^[1] Univ Estadual Campinas, Dept Food Engn, Lab Proc Engn, Sch Food Engn, Rua Monteiro Lobato 80, BR-13083862 Campinas, SP - Brazil

^[2] Univ Montpellier, Inst Europeen Membranes, ENSCM, CNRS, UM, CC 047, 2 Pl Eugene Bataillon, F-34095 Montpellier - France

^[3] Univ Estadual Campinas, Dept Food Engn, Lab High Pressure Food Engn, Sch Food Engn, Rua Monteiro Lobato 80, BR-13083862 Campinas, SP - Brazil

^[4] Univ Fed Rural Rio de Janeiro, Dept Food Technol, Rodovia BR 465, Km 7, BR-23890000 Seropedica, RJ - Brazil

^[5] Univ Fed Santa Catarina, Dept Chem & Food Engn, Lab Membrane Proc, BR-88040970 Florianopolis, SC - Brazil

• Document type: Journal article
• Source: Journal of Food Engineering; v. 302, AUG 2021.
• Web of Science Citations: 0

Abstract

This work investigated membrane selectivity and fouling after the dead-end ultrafiltration of protein hydrolysate from spent brewer's yeast, a high protein brewing by-product. Ultrafiltration (UF) experiments were carried out with regenerated cellulose (RC) and polyethersulfone (PES) membranes of 30 kDa molecular weight cut-off at different pH values (5 and 8). Higher peptide retention (60% of total solids and 83% of peptides) was achieved using PES and a feed pH of 5. Ribonucleic acids were successfully separated from the peptide-rich fraction. The protein hydrolysate at pH 8 showed less susceptibility to the adsorption of proteins and smaller resistance to mass transfer. Similarities in the zeta potential of membranes and feed solution suggested that foulants aggregated in the surface of membranes by weak physical adsorption. Peptides were confirmed by FT-IR analysis as the main foulants. Hydrophilic membranes and feed at pH 8 increased UF performance of spent brewer's yeast hydrolysate. (AU)

• FAPESP's process: 18/15737-2 - Passion fruit rind biorefinery: extraction of pectin and bioactive compounds and concentration of the extract with membranes
• Grantee: Débora Tamires Vitor Pereira
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 17/23670-2 - Biorefinery for the recovery of by-products from passion fruit processing through high pressure technologies
• Grantee: Julian Martínez
• Support Opportunities: Regular Research Grants
• FAPESP's process: 16/18465-8 - Membrane concentration and fracionation of brewer spent yeast protein hydrolyzate
• Grantee: Gabriela Vollet Marson
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 11/51707-1 - Understanding the emulsion behaviour of fermentation mixtures in the microbial production of diesel and jet-like biofuels
• Grantee: Rosiane Lopes da Cunha
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Regular Program Grants
• FAPESP's process: 18/04067-6 - Membrane concentration and fractionation of brewer spent yeast protein hydrolysate
• Grantee: Gabriela Vollet Marson
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate (Direct)
• FAPESP's process: 09/54137-1 - Acquisition of a particle size/distribution analyzer, a zeta potential measurement system, a spray dryer, and an ultra-high-pressure liquid chromatograph/mass spectrometer
• Grantee: Miriam Dupas Hubinger
• Support Opportunities: Multi-user Equipment Program
• FAPESP's process: 11/06083-0 - Emulsification by microchannels
• Grantee: Rosiane Lopes da Cunha
• Support Opportunities: Regular Research Grants