| Full text | |
| Author(s): |
Baldo, Thaisa Aparecida
[1]
;
Proenca, Camila dos Anjos
[1]
;
Felix, Fabiana da Silva
[2]
;
Freitas, Tayane Aguiar
[1]
;
Sakata, Solange Kazumi
[3]
;
Angnes, Lucio
[4]
;
Faria, Ronaldo Censi
[1]
Total Authors: 7
|
| Affiliation: | [1] Univ Fed Sao Carlos, Dept Quim, BR-13565905 Sao Carlos, SP - Brazil
[2] Univ Fed Lavras, Dept Quim, BR-37200900 Lavras, MG - Brazil
[3] Ctr Tecnol Radiacos, Inst Pesquisa Energet & Nucl IPEN CNEN SP, BR-05508000 Sao Paulo, SP - Brazil
[4] Univ Sao Paulo, Inst Quim, BR-05508000 Sao Paulo, SP - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | Talanta; v. 232, SEP 1 2021. |
| Web of Science Citations: | 1 |
| Abstract | |
Food allergies have been increasing all over the world. Egg is an important component in the food industries and the second most common cause of food allergy, shortly after milk. In the wine industry, egg white is applied as a fining agent for tannin removal. In this study, a sandwich-based immunoassay for ultrasensitive detection of ovalbumin (OVA) in wine samples was developed. The assay involves the use of magnetic beads (MBs) decorated with a polyclonal anti-OVA antibody (Ab(2)) and horseradish peroxidase (HRP), used as label for the quantification in a disposable electrochemical microfluidic device (DE mu D) here developed. The Ab(2)-MB-HRP prepared was applied to capture, separate, and pre-concentrate OVA from wine samples. In the DE mu D, OVA was immunemagnetically captured (OVA-Ab(2)-MB-HRP), producing a sandwich structure (GO-Ab(1)-OVA-Ab(2)-MB-HRP) on the electrode's surface. This arrangement results in an ultrasensitive device, achieving the ultralow limit of detection of 0.2 fg mL(-1) OVA. Five samples of wines were analyzed by using the immuno-magneto-assay which presents excellent accuracy compared with enzyme-linked immunosorbent assay (ELISA). (AU) | |
| FAPESP's process: | 13/14262-7 - Nanostructured films from biologically-relevant materials |
| Grantee: | Osvaldo Novais de Oliveira Junior |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 17/24053-7 - Development of simple electrochemical methods for the detection of biomarkers for the diagnosis of diseases |
| Grantee: | Ronaldo Censi Faria |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 16/00991-5 - Study of Detoxification Enzymes Interaction with chemotherapeutic drugs and its relationship with chemoresistance |
| Grantee: | Elsa María Materón Vásques |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 14/50867-3 - INCT 2014: National Institute of Science and Technology in Bioanalysis |
| Grantee: | Marco Aurelio Zezzi Arruda |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 17/13137-5 - Formation and properties of hemiesters of carbonic acid in aqueous medium |
| Grantee: | Claudimir Lucio Do Lago |
| Support Opportunities: | Research Projects - Thematic Grants |