Salmonella Typhimurium ST313 isolated in Brazil revealed to be more invasive and inflammatory in murine colon compared to ST19 strains

Author(s)

Seribelli, Amanda Aparecida ^[1] ; Machado Ribeiro, Tamara R. ^[2] ; da Silva, Patrick ^[2] ; Martins, Isabela Mancini ^[2] ; Vilela, Felipe Pinheiro ^[1] ; Cazentini Medeiros, I, Marta ; Peronni, Kamila Chagas ^[3] ; da Silva Junior, Wilson Araujo ^{[3, 4]} ; Moreira, Cristiano Gallina ^[2] ; Falcao, Juliana Pfrimer ^[1]

Affiliation

^[1] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, BR-05508220 Ribeirao Preto, SP - Brazil

^[2] Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, Dept Biol Sci, BR-01049010 Araraquara, SP - Brazil

^[3] Univ Sao Paulo, Reg Blood Ctr, Univ Hosp, Ribeirao Preto Med Sch, BR-05508220 Ribeirao Preto, SP - Brazil

^[4] Univ Sao Paulo, Ribeirao Preto Med Sch, Genet Dept, BR-05508220 Ribeirao Preto, SP - Brazil

• Document type: Journal article
• Source: JOURNAL OF MICROBIOLOGY; v. 59, n. 9 AUG 2021.
• Web of Science Citations: 0

Abstract

Salmonella Typhimurium (ST313) has caused an epidemic of invasive disease in sub-Saharan Africa and has been recently identified in Brazil. As the virulence of this ST is poorly understood, the present study aimed to (i) perform the RNA-seq in vitro of S. Typhimurium STm30 (ST313) grown in Luria-Bertani medium at 37 degrees C; (ii) compare it with the RNA-seq of the S. Typhimurium SL1344 (ST19) and S. Typhimurium STm11 (ST19) strains under the same growing conditions; and (iii) examine the colonization capacity and expression of virulence genes and cytokines in murine colon. The STm30 (ST313) strain exhibited stronger virulence and was associated with a more inflammatory profile than the strains SL1344 (ST19) and STm11 (ST19), as demonstrated by transcriptome and in vivo assay. The expression levels of the hilA, sopD2, pipB, and ssaS virulence genes, other Salmonella pathogenicity islands SPI-1 and SPI-2 genes or effectors, and genes of the cytokines IL-1 beta, IFN-gamma, TNF-alpha, IL-6, IL-17, IL-22, and IL-12 were increased during ST313 infection in C57BL/6J mice. In conclusion, S. Typhimurium STm30 (ST313) isolated from human feces in Brazil express higher levels of pathogenesis-related genes at 37 degrees C and has stronger colonization and invasion capacity in murine colon due to its high expression levels of virulence genes, when compared with the S. Typhimurium SL1344 (ST19) and STm11 (ST19) strains. STm30 (ST313) also induces stronger expression of pro-inflammatory cytokines in this organ, suggesting that it causes more extensive tissue damage. (AU)

• FAPESP's process: 17/06633-6 - Comparative analysis of the genome, transcriptome and phenotypic characterization of Salmonella typhimurium strains isolated from humans and food in Brazil
• Grantee: Amanda Aparecida Seribelli
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 16/12744-2 - Transcriptional YgiV role and VisP protein-protein interaction on chemical signaling and LPS assembly in Salmonella enterica sorovar Typhimurium pathogenesis
• Grantee: Patrick da Silva
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 19/19338-8 - Genomic, transcriptomic and phenotypic characterization of Campylobacter jejuni strains isolated from diverse source during 20 years in Brazil
• Grantee: Juliana Pfrimer Falcão
• Support Opportunities: Regular Research Grants
• FAPESP's process: 19/03049-7 - Transcriptome of the bacterial chemical signaling in Salmonella Typhimurium, emergent global bacterial enteropathogens investigation in São Paulo, and alternative infection model employed to study pathogenesis
• Grantee: Cristiano Gallina Moreira
• Support Opportunities: Regular Research Grants
• FAPESP's process: 16/24716-3 - Sequencing of the Genome, transcriptoma and phenotypic analysis of Campylobacter coli strains isolated from different sources in Brazil
• Grantee: Juliana Pfrimer Falcão
• Support Opportunities: Regular Research Grants