Pediocin PA-1 production by Pediococcus pentosaceus ET34 using non-detoxified hemicellulose hydrolysate obtained from hydrothermal pretreatment of sugarcane bagasse

Author(s):Show less -

Kuniyoshi, Tais Mayumi ^[1] ; Nobrega Mendonca, Carlos Miguel ^[1] ; Vieira, Viviane Borges ^[1] ; Robl, Diogo ^[2] ; Gombossy de Melo Franco, Bernadette Dora ^[3] ; Todorov, Svetoslav Dimitrov ^{[4, 5]} ; Tome, Elisabetta ^[6] ; O'Connor, Paula Mary ^{[7, 8]} ; Converti, Attilio ^[9] ; Araujo, Welington Luiz ^[10] ; Silva Palmeiras Vasconcellos, Livia Paula ^[11] ; Varani, Alessandro de Mello ^[12] ; Cotter, Paul David ^{[7, 8, 13]} ; Rabelo, Sarita Candida ^[14] ; de Souza Oliveira, Ricardo Pinheiro ^[1]

Affiliation:Show less -

^[1] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Biochem & Pharmaceut Technol, BR-05508000 Sao Paulo - Brazil

^[2] Fed Univ Santa Catarina UFSC, Dept Microbiol Immunol & Parasitol, BR-88040900 Florianopolis, SC - Brazil

^[3] Univ Sao Paulo, FoRC Food Res Ctr, Sch Pharmaceut Sci, Food & Expt Nutr Dept, BR-05508000 Sao Paulo - Brazil

^[4] Univ Sao Paulo, Sch Pharmaceut Sci, Food & Expt Nutr Dept, BR-05508000 Sao Paulo - Brazil

^[5] Handong Global Univ, ProBacLab, Adv Convergence, Gyeongbuk 37554 - South Korea

^[6] Univ Cent Venezuela, Inst Ciencias & Tecnol Alimentos, Escuela Biol, Apartado 47-097, Caracas 1041 - Venezuela

^[7] Teagasc Food Res Ctr, Food Biosci Dept, Moorepk, Cork T12 YN60 - Ireland

^[8] Univ Coll Cork, APC Microbiome Ireland, Cork T12 YT20 - Ireland

^[9] Genoa Univ, Dept Civil Chem & Environm Engn, I-16145 Genoa - Italy

^[10] Univ Sao Paulo, Microbiol Dept, Biomed Sci Inst, BR-05508900 Sao Paulo - Brazil

^[11] Natl Res Ctr Energy & Mat CNPEM, Brazilian Biorenewables Natl Lab LNBR, Campinas - Brazil

^[12] Sao Paulo State Univ, Fac Ciencias Agr & Vet, Sch Agr & Vet Sci, Dept Technol, BR-14884000 Jaboticabal, SP - Brazil

^[13] Univ Coll Cork, Sch Microbiol, Cork T12 YT20 - Ireland

^[14] Sao Paulo State Univ, Coll Agr Sci, Dept Bioproc & Biotechnol, BR-18610034 Sao Paulo - Brazil

• Document type: Journal article
• Source: APPLIED GEOCHEMISTRY; v. 131, AUG 2021.
• Web of Science Citations: 0

Abstract

Listeria monocytogenes is one of the foodborne pathogens of most concern for food safety. To limit its presence in foods, bacteriocins have been proposed as natural bio-preservatives. Herein, a bacteriocin was produced on hemicellulose hydrolysate of sugarcane bagasse by Pediococcus pentosaceous ET34, whose genome sequencing revealed an operon with 100% similarity to that of pediocin PA-1. ET34 grown on hydrolysate-containing medium led to an increase in the expression of PA-1 genes and a non-optimized purification step sequence resulted in a yield of 0.8 mg \& sdot;L-1 of pure pediocin (purity > 95%). Culture conditions were optimized according to a central composite design using temperature and hydrolysate % as independent variables and validated in 3-L Erlenmeyers. Finally, a process for scaled-up implementation by sugar-ethanol industry was proposed, considering green chemistry and biorefinery concepts. This work stands up as an approach addressing a future proper sugarcane bagasse valorisation for pediocin production. <comment>Superscript/Subscript Available</comment (AU)

• FAPESP's process: 18/04385-8 - Construction of a pediocin-producing recombinant lineage from agroindustrial waste
• Grantee: Ricardo Pinheiro de Souza Oliveira
• Support Opportunities: Regular Research Grants
• FAPESP's process: 18/25511-1 - Bioprospecting of bacteriocin-producing bacteria: from culture optimization to the application in animal production systems
• Grantee: Ricardo Pinheiro de Souza Oliveira
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 15/24777-0 - Optimization of pediocin A production in Pediococcus pentosaceus ATCC 43200
• Grantee: Taís Mayumi Kuniyoshi
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 13/07914-8 - FoRC - Food Research Center
• Grantee: Bernadette Dora Gombossy de Melo Franco
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC