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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Utilization of Clay Materials as Support for Aspergillus japonicus Lipase: An Eco-Friendly Approach

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Author(s):
Remonatto, Daniela [1] ; Ferrari, Barbara Ribeiro [1] ; Bassan, Juliana Cristina [1] ; Mussagy, Cassamo Ussemane [1, 2] ; Santos-Ebinuma, Valeria de Carvalho [1] ; de Paula, Ariela Veloso [1]
Total Authors: 6
Affiliation:
[1] Sao Paulo State Univ, Sch Pharmaceut Sci, Dept Engn Bioproc & Biotechnol, UNESP, BR-14800903 Araraquara, SP - Brazil
[2] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Pharmaceut Biochem Technol, BR-14000000 Ribeirao Preto - Brazil
Total Affiliations: 2
Document type: Journal article
Source: CATALYSTS; v. 11, n. 10 OCT 2021.
Web of Science Citations: 0
Abstract

Lipase is an important group of biocatalysts, which combines versatility and specificity, and can catalyze several reactions when applied in a high amount of industrial processes. In this study, the lipase produced by Aspergillus japonicus under submerged cultivation, was immobilized by physical adsorption, using clay supports, namely, diatomite, vermiculite, montmorillonite KSF (MKSF) and kaolinite. Besides, the immobilized and free enzyme was characterized, regarding pH, temperature and kinetic parameters. The most promising clay support was MKSF that presented 69.47% immobilization yield and hydrolytic activity higher than the other conditions studied (270.7 U g(-1)). The derivative produced with MKSF showed high stability at pH and temperature, keeping 100% of its activity throughout 12 h of incubation in the pH ranges between 4.0 and 9.0 and at a temperature from 30 to 50 \& DEG;C. In addition, the immobilized lipase on MKSF support showed an improvement in the catalytic performance. The study shows the potential of using clays as support to immobilized lipolytic enzymes by adsorption method, which is a simple and cost-effective process.</p> (AU)

FAPESP's process: 19/15493-9 - Development of ionic liquids-based electroconductive hydrogels
Grantee:Valéria de Carvalho Santos Ebinuma
Support Opportunities: Regular Research Grants
FAPESP's process: 20/08655-0 - Development of a sustainable and biocompatible technology for Astaxanthin recovery and purification: a powerful antioxidant with economic value
Grantee:Cassamo Ussemane Mussagy
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 18/06908-8 - Development of sustainable nanomaterials for the purification of antileukemic drugs
Grantee:Valéria de Carvalho Santos Ebinuma
Support Opportunities: Regular Research Grants
FAPESP's process: 20/09592-1 - Nanoencapsulated bioactive esters: enzymatic synthesis and study of potential antimicrobial
Grantee:Ariela Veloso de Paula
Support Opportunities: Regular Research Grants
FAPESP's process: 17/11482-7 - Enzymatic synthesis of dietary triglycerides employing Airlift, fixed bed and fluidized bed reactors
Grantee:Ariela Veloso de Paula
Support Opportunities: Regular Research Grants