Foliar spraying of biogenic CuO nanoparticles protects the defence system and photosynthetic pigments of lettuce (Lactuca sativa)

Author(s)

Kohatsu, Marcio Yukihiro ^[1] ; Lange, Camila Neves ^[2] ; Pelegrino, Milena Trevisan ^[2] ; Pieretti, Joana Claudio ^[2] ; Tortella, Gonzalo ^[3] ; Rubilar, Olga ^[3] ; Batista, Bruno Lemos ^[2] ; Seabra, Amedea Barozzi ^[2] ; de Jesus, Tatiane Araujo ^{[1, 4]}

Affiliation

^[1] Univ Fed ABC UFABC, Programa Posgrad Ciencia & Tecnol Ambiental CTA, Avenida Estados, 5001 Bairro Santa Terezinha, BR-09210580 Santo Andre, SP - Brazil

^[2] Univ Fed ABC UFABC, Ctr Ciencias Nat & Humanas, Avenida Estados, 5001 Bairro Santa Terezinha, BR-09210580 Santo Andre, SP - Brazil

^[3] Univ La Frontera, Dept Chem Engn, Biotechnol Res Ctr Appl Environm CIBAMA BIOREN, Temuco - Chile

^[4] Univ Fed ABC UFABC, Ctr Engn Modelagem & Ciencias Sociais Aplicadas, Ave Estados, 5001 Bairro Santa Terezinha, BR-09210580 Santo Andre, SP - Brazil

• Document type: Journal article
• Source: JOURNAL OF CLEANER PRODUCTION; v. 324, NOV 15 2021.
• Web of Science Citations: 0

Abstract

Engineered nanoparticles (NPs) can act as biostimulants (fertilizers) or cause toxicity in plants (phytotoxicity) depending on several factors. When toxicity is produced, it can lead to oxidative stress, disturbing the plant's oxidative defence system. Biogenic NPs, obtained by green synthesis, are an interesting alternative which obviates toxicity and oxidative stress-related effects. The object of this study was to evaluate how biogenic CuO NPs synthesized with green tea (20 mg per plant) influence the defence system and the photosynthetic pigment content of Lactuca sativa in pot experiments under greenhouse conditions. Moreover, the exposure routes (foliar spraying and soil irrigation) and the forms of Cu applied (nanoscale and ionic) were compared. The defence system of L. sativa was accessed by antioxidant enzymatic activity {[}ascorbate peroxidase (APX), catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD)]; secondary metabolites or antioxidant non enzymatic activity (total flavonoids and phenolic compounds); photosynthetic pigments {[}chlorophyll a and b and carotenoids]; and nitric oxide levels {[}R-S-nitrosothiols (RSNOs) and nitrite (NO2-)]. Foliar spraying of CuO NPs reduced APX, but there were no variations in CAT, POD, or SOD activities or photosynthetic pigment contents. However, levels of RSNOs and secondary metabolites increased. In contrast, treatments with CuSO4 raised the APX level and reduced levels of RSNOs, chlorophyll b and carotenoids. Soil irrigation with CuO NPs did not increase enzymatic activity, however it reduced the chlorophyll a and b contents. The use of biogenic CuO NPs via foliar spray showed promising results in protecting lettuce from oxidative stress. (AU)

• FAPESP's process: 19/26337-8 - Effects of copper oxide nanoparticles using in vitro and in vivo bioassays with lettuce (Lactuca sativa L.)
• Grantee: Marcio Yukihiro Kohatsu
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 17/20914-8 - Method validation for determination of trace elements in urine for occupational evaluation of foundry workers
• Grantee: Bruna Moreira Freire
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 17/05029-8 - Preparation, characterization and biological evaluation of nitric oxide releasing polymeric biomaterials
• Grantee: Milena Trevisan Pelegrino
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 21/00738-6 - Synthesis of copper and silver nanostructured complexes to replace growth-promoting antibiotics in pig and poultry production
• Grantee: Camila Neves Lange
• Support Opportunities: Scholarships in Brazil - Innovative Research in Small Business - PIPE
• FAPESP's process: 18/08194-2 - Essential oil amended with metal nanoparticles functionalized with nitric oxide as a strategy to control plant pathogens in the agriculture
• Grantee: Amedea Barozzi Seabra
• Support Opportunities: Regular Research Grants
• FAPESP's process: 16/10060-9 - Multi-user equipment (MUE) approved in Grant FAPESP project 2014/05151-0: inductively coupled plasma mass spectrometer hyphenated to an high performance liquid chromatography (HPLC-ICP-MS)
• Grantee: Bruno Lemos Batista
• Support Opportunities: Multi-user Equipment Program