Flexible, Bifunctional Sensing Platform Made with Biodegradable Mats for Detecting Glucose in Urine

Author(s)

Gomes, Nathalia O. ; Paschoalin, Rafaella T. ; Bilatto, Stanley ; Sorigotti, Amanda R. ; Farinas, Cristiane S. ; Mattoso, Luiz Henrique C. ; Machado, Sergio A. S. ; Oliveira Jr, Osvaldo N. ; Raymundo-Pereira, Paulo A.

• Document type: Journal article
• Source: ACS SUSTAINABLE CHEMISTRY & ENGINEERING; v. 11, n. 6, p. 10-pg., 2023-02-13.

Abstract

We report on a flexible, bifunctional platform made with solution-blow spinning fiber mats of polylactic acid (PLA) and polyethylene glycol (PEG). The PLA/ PEG mats were designated bifunctional because they were used as a substrate for screen -printed carbon electrodes (SPCEs) and as a matrix to attach glucose oxidase (GOx) covalently. By attaching GOx only on the fiber mats, and not on the SPCE, the detection of hydrogen peroxide generated from glucose oxidation by the enzyme was efficient, especially with Prussian blue (PB) nanoparticles electrodeposited on the SPCE surface. The bifunctional device detected hydrogen peroxide (H2O2) in synthetic and human urine at a low applied potential (0 V vs Ag/AgCl) in the range from 1.0 to 6.0 mM, with a detection limit of 0.197 mM, being also interference-free. The biosensor whose cost is <US$ 0.25 per unit (estimated from the price of the materials used) had performance comparable to the gold standard method while measuring glucose in human urine samples, in addition to exhibiting a rapid response and long lifetime. The design of the bifunctional device is generic and may be extended to other biosensors for on-site monitoring as well as for wearable devices and in monitoring the environment and water in varied resources. (AU)

• FAPESP's process: 19/01777-5 - Manufacture of disposable electrochemical devices composed of hollow carbon nanospheres and carbon nanowires decorated with silver nanoparticles for the quantification of endocrine disruptors in supply water samples
• Grantee: Sergio Antonio Spinola Machado
• Support Opportunities: Regular Research Grants
• FAPESP's process: 20/09587-8 - Development of electrochemical devices based on carbon and silver nanostructures to detect endocrine disruptors in quantities of water supply
• Grantee: Nathalia Oezau Gomes
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 16/10636-8 - From the cell factory to the Biodiesel-Bioethanol integrated biorefinery: a systems approach applied to complex problems in micro and macroscales
• Grantee: Roberto de Campos Giordano
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Thematic Grants
• FAPESP's process: 17/18725-2 - Hybrid core-shell and Janus nanofibers obtained by solution blow spinning
• Grantee: Rafaella Takehara Paschoalin
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 18/22214-6 - Towards a convergence of technologies: from sensing and biosensing to information visualization and machine learning for data analysis in clinical diagnosis
• Grantee: Osvaldo Novais de Oliveira Junior
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 13/07296-2 - CDMF - Center for the Development of Functional Materials
• Grantee: Elson Longo da Silva
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
• FAPESP's process: 18/10899-4 - Utilization of residual lignocellulosic biomass from 2G ethanol processing to obtain nanocellulose via enzymatic route
• Grantee: Stanley Endrigo Bilatto Rodrigues
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 22/02164-0 - Development of electrochemical sensors modified with hollow carbon nanospheres doped with sulfur and nitrogen for the quantification of drugs in supply water samples
• Grantee: Sergio Antonio Spinola Machado
• Support Opportunities: Regular Research Grants
• FAPESP's process: 16/01919-6 - Design and fabrication of nanostructured flexible devices for biomarkers detection
• Grantee: Paulo Augusto Raymundo Pereira
• Support Opportunities: Scholarships in Brazil - Post-Doctoral