In Vitro Evaluation of Photodynamic Activity of Plant Extracts from Senna Species against Microorganisms of Medical and Dental Interest

Author(s)

de Oliveira, Analu Barros ; Ferrisse, Tulio Morandin ; de Annunzio, Sarah Raquel ; Franca, Maria Gleiziane Araujo ; Silva, Maria Goretti de Vasconcelos ; Cavalheiro, Alberto Jose ; Fontana, Carla Raquel ; Brighenti, Fernanda Lourencao

• Document type: Journal article
• Source: PHARMACEUTICS; v. 15, n. 1, p. 16-pg., 2023-01-01.

Abstract

Background: Bacterial resistance requires new treatments for infections. In this context, antimicrobial photodynamic therapy (aPDT) is an effective and promising option. Objectives: Three plant extracts (Senna splendida, Senna alata, and Senna macranthera) were evaluated as photosensitizers for aPDT. Methods: Cutibacterium acnes (ATCC 6919), Streptococcus mutans (ATCC 35668), Staphylococcus aureus (ATCC 25923), Escherichia coli (ATCC 25922), and Candida albicans (ATCC 90028) were evaluated. Reactive oxygen species production was also verified. Oral keratinocytes assessed cytotoxicity. LC-DAD-MS analysis identified the chemical components of the evaluated extracts. Results: Most species cultured in the planktonic phase showed total microbial reduction (>6 log10 CFU/mL/p < 0.0001) for all extracts. C. albicans cultured in biofilm showed total microbial reduction (7.68 log10 CFU/mL/p < 0.0001) for aPDT mediated by all extracts. Extracts from S. macranthera and S. alata produced the highest number of reactive oxygen species (p < 0.0001). The S. alata extract had the highest cell viability. The LC-DAD-MS analysis of active extracts showed one naphthopyrone and seven anthraquinones as potential candidates for photoactive compounds. Conclusion: This study showed that aPDT mediated by Senna spp. was efficient in microbial suspension and biofilm of microorganisms of medical and dental interest. (AU)

• FAPESP's process: 13/07600-3 - CIBFar - Center for Innovation in Biodiversity and Drug Discovery
• Grantee: Glaucius Oliva
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
• FAPESP's process: 22/12828-2 - Interaction of Candida streptococcus multispecies biofilm in dental demineralization and oral candidosis
• Grantee: Túlio Morandin Ferrisse
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• FAPESP's process: 14/50926-0 - INCT 2014: biodiversity and natural products
• Grantee: Vanderlan da Silva Bolzani
• Support Opportunities: BIOTA-FAPESP Program - Thematic Grants
• FAPESP's process: 20/07110-0 - Potential of photodynamic therapy in modulating the inflammatory response and microbial virulence factors: evaluation in a model of three-dimensional co-cultures of gingival fibroblasts, Candida albicans and Staphylococcus aureus
• Grantee: Analú Barros de Oliveira
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 19/08375-0 - Maximization of the anti-caries and antibiofilm effect of conventional substances: evaluation in a polymicrobial biofilm model
• Grantee: Fernanda Lourenção Brighenti
• Support Opportunities: Regular Research Grants
• FAPESP's process: 18/09088-1 - Assessment of photodynamic therapy against polymicrobial acne biofilms: a preclinical study
• Grantee: Sarah Raquel de Annunzio
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 18/18440-0 - Bioprospection of natural products in photodynamic therapy against microorganisms of medial and dental importance
• Grantee: Analú Barros de Oliveira
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 18/23015-7 - Antimicrobial photodynamic therapy by continuous and switched mode irradiation against Enterococcus faecalis and Cutibacterium acnes
• Grantee: Carla Raquel Fontana
• Support Opportunities: Regular Research Grants
• FAPESP's process: 21/01191-0 - Tolerance induction and Candida albicans's biofilm characterization after antimicrobial photodymamic therapy application
• Grantee: Túlio Morandin Ferrisse
• Support Opportunities: Scholarships in Brazil - Post-Doctoral