| Full text | |
| Author(s): |
Bianchi, Fernanda
;
Larsen, Nadja
;
Tieghi, Thatiana de Mello
;
Adorno, Maria Angela T.
;
Saad, Susana M., I
;
Jespersen, Lene
;
Sivieri, Katia
Total Authors: 7
|
| Document type: | Journal article |
| Source: | Food Research International; v. 120, p. 8-pg., 2019-06-01. |
| Abstract | |
The gut microbiota composition and its metabolites have high impact on human health. Exploitation of prebiotics and probiotics for modulation of gut microbiota can lead to promising outcomes. This study aimed to evaluate the effects of the probiotic strain Bifidobacterium longum BB-46 alone and in combination with a citric pectin from lemon on the gut microbiota from healthy adults using the Simulator of Human Intestinal Microbial Ecosystem (SHIME center dot). Changes in microbiota composition and in metabolic activity were assessed by the 16S rRNA gene sequencing and by analyses of short-chain fatty acids (SCFAs) and ammonium ions (NH4+). An increase in the relative abundances of Firmicutes (especially the members of Lachnospiraceae and Lactobacillaceae families) and Bacteroidetes was observed during treatment with B. longum BB-46 alone in all compartments of the colon. Treatment with B. longum BB-46 and pectin stimulated an increase in the proportions of genera Faecalibacteriwn, Eubacterium and Lactobacillus, as well as in the Ruminococcaceae family in the transverse and descending colons. Concurrently, the butyrate levels increased in these two compartments. Additionally, the combination of B. longum BB-46 and pectin reduced the abundance of proteolytic bacteria Bacteroides, Clostridium, Peptoniphilus, and Streptococcus, along with decreased NH4+ production. No significant changes could be observed on NH4+ production by treatment with B. longum BB-46, nor did it increase the amount of SCFAs. In this study, we observed that although each treatment was able to modulate the microbiota, the combination of B. longum BB-46 and pectin was more efficient in decreasing the intestinal NH(4)(+)d levels and in increasing butyric acid-producing bacteria. These findings indicate that B. longwn BB-46, especially when combined with the specific citric pectin, might have beneficial impact on human health. (AU) | |
| FAPESP's process: | 16/20336-1 - Effect of synbiotic activity on intestinal epithelial integrity in obese and lean microbiota |
| Grantee: | Fernanda Bianchi |
| Support Opportunities: | Scholarships abroad - Research Internship - Doctorate |
| FAPESP's process: | 13/50506-8 - Bioactive components from by-products of food processing used in a symbiotic approach for improving human health and well-being (BioSyn) |
| Grantee: | Susana Marta Isay Saad |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 15/13965-0 - Selection and assessment of the combination between waste of tropical fruits and probiotic strains in the modulation of intestinal microbiota of obese compared to the normal weight using the simulator of the human intestinal microbial ecosystem |
| Grantee: | Katia Sivieri |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 15/08228-6 - Selection and assessment of the combination between waste of tropical fruits and probiotic strains in the modulation of intestinal microbiota of obese compared to the normal weight using the simulator of the human intestinal microbial ecosystem |
| Grantee: | Fernanda Bianchi |
| Support Opportunities: | Scholarships in Brazil - Doctorate |