3D-printed immunosensor for the diagnosis of Parkinson?s disease

Author(s)

Kalinke, Cristiane ; De Oliveira, Paulo Roberto ; Banks, Craig E. ; Janegitz, Bruno Campos ; Bonacin, Juliano Alves

• Document type: Journal article
• Source: SENSORS AND ACTUATORS B-CHEMICAL; v. 381, p. 8-pg., 2023-01-27.

Abstract

3D printing technology is a strategic tool for the development of electrochemical sensors and biosensors since it is possible to obtain versatile devices quickly and at a low cost. In this work, an arrangement of 3D-printed electrodes (working, pseudo-reference, and auxiliary) was applied for the detection of PARK7/DJ-1 protein in blood serum and cerebrospinal fluid samples. The immunosensor surface was previously chemically and elec-trochemically activated to promote the increase of the active sites and the conductivity, allowing the covalent immobilization of the biological species (antibodies) and improving its electrochemical performance. The detection was carried out by impedimetric (5.0-200 mu g L-1), and voltammetric measurements (5.0-500 mu g L-1), showing limits of detection of 1.01 and 3.46 mu g L-1. The 3D-printed immunosensor also achieved good repeatability and reproducibility from normal to abnormal levels of PARK7/DJ-1 protein, aiming for the diag-nosis of Parkinson's disease in different stages of the disease. (AU)

• FAPESP's process: 19/01844-4 - Development of nanostructured electrodes for determination of agrotoxic residues in honey
• Grantee: Paulo Roberto de Oliveira
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 19/00473-2 - Development of 3-dimensional (3D) printed electrochemical biosensors with PLA polymer and graphene for the determination of biomolecules and diagnosis of diseases
• Grantee: Cristiane Kalinke
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 17/21097-3 - Bee-agriculture interactions: perspectives to sustainable use
• Grantee: Osmar Malaspina
• Support Opportunities: BIOTA-FAPESP Program - Thematic Grants
• FAPESP's process: 21/07989-4 - Additive-manufactured (3D printed) electrochemical devices for the diagnosis of viral diseases
• Grantee: Cristiane Kalinke
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• FAPESP's process: 13/22127-2 - Development of novel materials strategic for integrated analytical devices
• Grantee: Lauro Tatsuo Kubota
• Support Opportunities: Research Projects - Thematic Grants