Fast and green universal method to analyze and quantify anthocyanins in natural products by UPLC-PDA

Author(s)

Mesquita, Leonardo M. de Souza ; Contieri, Leticia S. ; Sanches, Vitor L. ; Kamikawachi, Renan ; Sosa, Filipe H. B. ; Vilegas, Wagner ; Rostagno, Mauricio A.

• Document type: Journal article
• Source: Food Chemistry; v. 428, p. 11-pg., 2023-12-01.

Abstract

This work developed a universal UPLC-PDA method based on safe reagents to analyze anthocyanins from different foods. Nine foods were studied by the developed chromatographic method, which was constructed using a solid core C18 column and a binary mobile phase composed of (A) water (0.25 molcitric acid.Lsolvent  1 ), and (B) ethanol. A total running time of 6 min was obtained, the faster comprehensive method for anthocyanins analysis. Mass spectrometry analysis was employed to identify a comprehensive set of 53 anthocyanins comprising glycosylated and acylated cyanidin, pelargonidin, malvidin, peonidin, petunidin, and delphinidin derivatives. Cyanidin-3-O-glucoside (m/z+ 449) and cyanidin-3-O-rutinoside (m/z+ 595) were used as standards to validate the accuracy of the developed method. The analytical parameters were evaluated, including intra-day and inter-day precision, robustness, repeatability, retention factor (k), resolution, and peak symmetry factor. The current method demonstrated excellent chromatographic resolution, making it a powerful tool for analyzing anthocyanins pigments. (AU)

• FAPESP's process: 21/11022-1 - Integrated biorefinery of agricultural wastes to obtain natural dyes and value-added bioactive compounds
• Grantee: Leonardo Mendes de Souza Mesquita
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• FAPESP's process: 18/14582-5 - Development of a two-dimensional extraction, separation and analysis system for evaluating the bioactivity of phytochemicals
• Grantee: Maurício Ariel Rostagno
• Support Opportunities: Research Grants - Young Investigators Grants - Phase 2
• FAPESP's process: 20/08421-9 - Application of non-volatile alternative solvents to create sustainable two-dimensional and integrated platforms for harnessing agro-industrial waste from jaboticaba and pomegranate fruits
• Grantee: Leonardo Mendes de Souza Mesquita
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 19/13496-0 - Multi user equipment approved in grant 2018/14582-5: ultra-high pressure liquid chromatography system (UHPLC), composed by a quaternary pump, autoinjector, oven with selection valve and detector (PDA)
• Grantee: Maurício Ariel Rostagno
• Support Opportunities: Multi-user Equipment Program
• FAPESP's process: 20/03623-2 - Extraction and separation of phenolic compounds from green propolis by a bidimensional system
• Grantee: Letícia Sanches Contieri
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 21/11023-8 - Development of sustainable methods for propolis extracts by the application of non-volatile alternative solvents
• Grantee: Letícia Sanches Contieri
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate (Direct)