How do pH and temperature influence extraction yield, physicochemical, functional, and rheological characteristics of brewer spent grain protein concentrates?

Author(s)

da Silva, Aurenice Maria Mota ; Almeida, Flavia Souza ; da Silva, Marcos Fellipe ; Goldbeck, Rosana ; Sato, Ana Carla Kawazoe

• Document type: Journal article
• Source: FOOD AND BIOPRODUCTS PROCESSING; v. 139, p. 12-pg., 2023-05-01.

Abstract

In this study, brewer spent grain (BSG) proteins were obtained by one-stage alkaline ex-traction and the influence of extraction pH and temperature on protein extraction yield, physicochemical, functional, and rheological characteristics were assessed. Under the optimized conditions (pH 11, 60 degrees C and solid/solvent ratio of 1/17 g/mL), up to 87% of the proteins were extracted. Mild alkaline (pH 8) extraction resulted in extracts with higher protein content (> 40%) that were more soluble and with better emulsifying properties compared to extraction at extreme alkaline conditions (pH 11 and 12). Increasing the extraction pH to 11 or the temperature to 80 degrees C resulted in BSG protein concentrates (BSG-PCs) with lower protein content (similar to 30% protein) (p < 0.05) and slightly less soluble. However, the BSG-PCs obtained under more severe conditions presented the largest in-creases in G' and G" during the temperature sweep test, indicating that the changes caused by the process could have improved the gelling properties of these proteins. BSG-PC is a promising plant protein that could be used as an alternative for the development of different food products.(c) 2023 Published by Elsevier Ltd on behalf of Institution of Chemical Engineers. (AU)

• FAPESP's process: 19/08542-3 - Enzymatic biotechnology for the xylo-oligosaccharides production from lignocellulosic agroindustrial residues aimed the production of symbiotics
• Grantee: Rosana Goldbeck Coelho
• Support Opportunities: Regular Research Grants
• FAPESP's process: 18/21987-1 - Obtainment and functionality of non-conventional plant based proteins as encapsulation matrices
• Grantee: Ana Carla Kawazoe Sato
• Support Opportunities: Regular Research Grants
• FAPESP's process: 11/06083-0 - Emulsification by microchannels
• Grantee: Rosiane Lopes da Cunha
• Support Opportunities: Regular Research Grants
• FAPESP's process: 09/54137-1 - Acquisition of a particle size/distribution analyzer, a zeta potential measurement system, a spray dryer, and an ultra-high-pressure liquid chromatograph/mass spectrometer
• Grantee: Miriam Dupas Hubinger
• Support Opportunities: Multi-user Equipment Program
• FAPESP's process: 15/11984-7 - PRODUCTION OF MICROPARTICLES CONTAINING FAT-SOLUBLE ACTIVE CORES BY ATOMIZATION IN SPRAY DRYING: EFFECT OF EMULSIONS PROPERTIES ON THE CHARACTERISTICS OF THE FINAL PRODUCT
• Grantee: Miriam Dupas Hubinger
• Support Opportunities: Regular Research Grants
• FAPESP's process: 19/27354-3 - Architecture of colloidal delivery systems: what is the role of structure on the digestibility?
• Grantee: Rosiane Lopes da Cunha
• Support Opportunities: Research Projects - Thematic Grants