Pressurized liquid extraction assisted by high-intensity ultrasound to obtain hesperidin from lime waste: Integration of in-line purification and on-line chromatographic analysis

Author(s):Show less -

Sanches, Vitor L. ; Strieder, Monique Martins ; Breitkreitz, Marcia Cristina ; Bezerra, Rosangela Maria Neves ; Chaves, Jaisa O. ; de Oliveira, Isadora Lopes ; Mesquita, Leonardo M. de Souza ; Vigano, Juliane ; Contieri, Leticia Sanches ; Pizani, Rodrigo Stein ; Rostagno, Mauricio A.

• Document type: Journal article
• Source: Food Research International; v. 182, p. 9-pg., 2024-02-28.

Abstract

Hesperidin is a phenolic compound usually found in citrus fruits, which is known for its anti-inflammatory and antioxidant properties. This bioactive compound has already been used to formulate medications to treat chronic venous insufficiency. In this work, through a system which allows the in-line coupling of the pressurized liquid extraction (PLE) and high-intensity ultrasound (HIUS) with solid phase extraction (SPE), and analysis by high-performance liquid chromatography with UV-Vis detector (HPLC-UV) in on-line mode, a method was developed to obtain, separate, and quantify hesperidin from the industrial waste of lime. An eco-friendly approach with water and ethanol as extraction solvents was used. Parameters such as temperature (80, 100, and 120 degrees C) and HIUS power (0, 200, and 400 W) were evaluated regarding hesperidin yield. In this context, the higher hesperidin yield (18.25 +/- 1.52 mg/g) was achieved using water at a subcritical state (120 degrees C and 15 MPa). The adsorbent Sepra (TM) C-18-E isolated hesperidin from the other extracted compounds employing 50% ethanol in the SPE elution. The possibility of on-line analysis coupling a high-performance liquid chromatograph to an ultraviolet detector (HPLC-UV) system was studied and shown to be a feasible approach for developing integrated technologies. Conventional extractions and their antioxidant capacities were evaluated, highlighting the advantages of the HIUS-PLE-SPE extractive method. Furthermore, the on-line chromatographic analysis showed the potential of the HIUS-PLE-SPE- HPLC-UV system to quantify the extracted compounds in real time. (AU)

• FAPESP's process: 20/08421-9 - Application of non-volatile alternative solvents to create sustainable two-dimensional and integrated platforms for harnessing agro-industrial waste from jaboticaba and pomegranate fruits
• Grantee: Leonardo Mendes de Souza Mesquita
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 19/24537-0 - Phytochemical evaluation of apple (Malus domestica) industrial residue from extraction and separation by a two-dimensional system
• Grantee: Laise Capelasso da Silva
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 20/03623-2 - Extraction and separation of phenolic compounds from green propolis by a bidimensional system
• Grantee: Letícia Sanches Contieri
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 19/13496-0 - Multi user equipment approved in grant 2018/14582-5: ultra-high pressure liquid chromatography system (UHPLC), composed by a quaternary pump, autoinjector, oven with selection valve and detector (PDA)
• Grantee: Maurício Ariel Rostagno
• Support Opportunities: Multi-user Equipment Program
• FAPESP's process: 21/13638-0 - Development of active packaging through the extraction of bioactive compounds from pomegranate peel (Punica granatum L.)
• Grantee: Isadora Lopes de Oliveira
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 18/14582-5 - Development of a two-dimensional extraction, separation and analysis system for evaluating the bioactivity of phytochemicals
• Grantee: Maurício Ariel Rostagno
• Support Opportunities: Research Grants - Young Investigators Grants - Phase 2