Pressurized liquid extraction of bioactive compounds from grape peel and application in pH-sensing carboxymethyl cellulose films: A promising material to monitor the freshness of pork and milk

Author(s)

Francisco, Ana Paula ; Sganzerla, William Gustavo ; Castro, Luiz Eduardo Nochi ; Barroso, Tiago Linhares Cruz Tabosa ; da Silva, Aline Priscilla Gomes ; da Rosa, Cleonice Goncalves ; Nunes, Michael Ramos ; Forster-Carneiro, Tania ; Rostagno, Mauricio A.

• Document type: Journal article
• Source: Food Research International; v. 179, p. 12-pg., 2024-01-19.

Abstract

This study produced pH-sensing carboxymethyl cellulose (CMC) films functionalized with bioactive compounds obtained by pressurized liquid extraction (PLE) of grape peel to monitor the freshness of pork and milk. A semi-continuous PLE was conducted using hydroethanolic solution (70:30, v/v) at a flow rate of 5 mL/min, 15 MPa, and 60 degrees C. The films were produced by the casting technique using CMC (2.5 %, w/v), glycerol (1 %, v/v), and functionalized with 10, 30, and 50 % (v/v) grape peel extract. From the results obtained, LC-MS/MS revealed that PLE extracted twenty-seven phenolic compounds. The main phenolic compounds were kaempferol-3-glucoside (367.23 +/- 25.88 mu g/mL), prunin (270.23 +/- 3.62 mu g/mL), p-coumaric acid (236.43 +/- 26.02 mu g/mL), and procyanidin B1 (117.17 +/- 7.29 mu g/mL). The CMC films presented suitable color and mechanical properties for food packaging applications. The addition of grape peel extract promoted the pH-sensing property, showing the sensitivity of anthocyanins to pH changes. The films functionalized with grape peel extract presented good release control of bioactive compounds, making them suitable for food packaging applications. When applied to monitor the freshness of pork and milk, the films exhibited remarkable color changes associated with the pH of the food during storage. In conclusion, PLE is a sustainable approach to obtaining bioactive compounds from the grape peel, which can be applied in the formulation of pH-sensing films as a promising sustainable material to monitor food freshness during storage. (AU)

• FAPESP's process: 19/26925-7 - Development of an automated system for biofuel precursor production using sub-critical technology
• Grantee: William Gustavo Sganzerla
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 21/04096-9 - Integration of the extraction/hydrolysis processes in subcritical water and purification for the valuation of grape bagasse in a biorrefinery concept
• Grantee: Luiz Eduardo Nochi de Castro
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 20/16248-5 - Purification of jabuticaba waste extracts by adsorbents and biochar using supercritical technology
• Grantee: Tiago Linhares Cruz Tabosa Barroso
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 18/14582-5 - Development of a two-dimensional extraction, separation and analysis system for evaluating the bioactivity of phytochemicals
• Grantee: Maurício Ariel Rostagno
• Support Opportunities: Research Grants - Young Investigators Grants - Phase 2