| Full text | |
| Author(s): |
Scaketti, Matheus
;
Sujii, Patricia Sanae
;
Alves-Pereira, Alessandro
;
Schwarcz, Kaiser Dias
;
Francisconi, Ana Flavia
;
Moro, Matheus Sartori
;
Martins, Kauanne Karolline Moreno
;
de Jesus, Thiago Araujo
;
de Souza, Guilherme Brener Ferreira
;
Zucchi, Maria Imaculada
Total Authors: 10
|
| Document type: | Journal article |
| Source: | PLoS One; v. 20, n. 2, p. 14-pg., 2025-02-13. |
| Abstract | |
Obtaining large sample sizes for genetic studies can be challenging, time-consuming, and expensive, and small sample sizes may generate biased or imprecise results. Many studies have suggested the minimum sample size necessary to obtain robust and reliable results, but it is not possible to define one ideal minimum sample size that fits all studies. Here, we present SaSii (Sample Size Impact), an R script to help researchers define the minimum sample size. Based on empirical and simulated data analysis using SaSii, we present patterns and suggest minimum sample sizes for experiment design. The patterns were obtained by analyzing previously published genotype datasets with SaSii and can be used as a starting point for the sample design of population genetics and genomic studies. Our results showed that it is possible to estimate an adequate sample size that accurately represents the real population without requiring the scientist to write any program code, extract and sequence samples, or use population genetics programs, thus simplifying the process. We also confirmed that the minimum sample sizes for SNP (single-nucleotide polymorphism) analysis are usually smaller than for SSR (simple sequence repeat) analysis and discussed other patterns observed from empirical plant and animal datasets. (AU) | |
| FAPESP's process: | 13/00003-0 - Genetic diversity, genomics and phylogeography of manioc (Manihot esculenta Crantz): implications for the dispersion and diffusion of the crop along the main fluvial axes in Brazilian Amazon Basin |
| Grantee: | Maria Imaculada Zucchi |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 11/50296-8 - Conservation biology of Atlantic Forest’s medicinal species: a genetical approach to Forest restorations |
| Grantee: | Maria Imaculada Zucchi |
| Support Opportunities: | BIOTA-FAPESP Program - Regular Research Grants |
| FAPESP's process: | 12/06431-0 - Genetic diversity and mating system of Myroxylon peruiferum, a species with phytoterapic potential, aiming the genetic enrichment in the area in forest restoration process. |
| Grantee: | Ellida de Aguiar Silvestre |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
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| Grantee: | João Paulo Gomes Viana |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
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| Grantee: | Kaiser Dias Schwarcz |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 12/03246-8 - Genetic diversity and structure and reproductive system in Centrolobium tomentosum Guillem. Bentham (Fabaceae) aiming to support genetic enrichment in areas of forest restoration |
| Grantee: | Patricia Sanae Sujii |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
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| Grantee: | Elizabeth Ann Veasey |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 14/01364-9 - A genetic approach for simulating persistence of reintroduced tree species populations in tropical forest restoration plantings |
| Grantee: | Patricia Sanae Sujii |
| Support Opportunities: | Scholarships abroad - Research Internship - Doctorate |
| FAPESP's process: | 13/08086-1 - Population and functional genomics and an ecophysiological approach on the evolutionary study of neotropical mangrove species in face of historical and current climate changes |
| Grantee: | Gustavo Maruyama Mori |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 18/00036-9 - Genomic resources of manioc (Manihot esculenta) for the evolutionary study of cultivated varieties and in the search of important genes for breeding |
| Grantee: | Alessandro Alves Pereira |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 11/21295-3 - Divesity, Genetic Structure and Population Genomics of Centrolobium tomentosum in natives and restaured áreas of Brazilian Atlantic Forest. |
| Grantee: | Camila Menezes Trindade Macrini |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 13/11137-7 - Genetic diversity, genomics and phylogeography of manioc (Manihot esculenta Crantz): implications for the dispersion of the crop along the main fluvial axes in Brazilian Amazon basin |
| Grantee: | Alessandro Alves Pereira |
| Support Opportunities: | Scholarships in Brazil - Doctorate |