| Grant number: | 09/54014-7 |
| Support Opportunities: | Multi-user Equipment Program |
| Start date: | October 01, 2010 |
| End date: | March 31, 2014 |
| Field of knowledge: | Biological Sciences - Biochemistry - Molecular Biology |
| Principal Investigator: | Enilza Maria Espreafico |
| Grantee: | Enilza Maria Espreafico |
| Host Institution: | Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil |
| As informações de acesso ao Equipamento Multiusuário são de responsabilidade do Pesquisador responsável | |
| EMU web page: | Página do Equipamento Multiusuário não informada |
| Type of equipment: | Tipo de Equipamento Multiusuário não informado |
| Manufacturer: | Fabricante não informado |
| Model: | Modelo não informado |
Abstract
The objective of the present request is to fill a major gap in the availability of in vivo imaging methodologies in the state of São Paulo, through the acquisition of two modern pieces of equipment in this area: the IVIS Spectrum biophotonic in vivo imaging system (Caliper Life Sciences) and the LSM 780 NLO multiphoton microscopy system for intravital imaging (Carl Zeiss). This equipment will be installed in the currently functioning Center for Cell and Tissue Imaging of the Department of Cell and Molecular Biology and Pathogenic Bioagents of the USP Ribeirão Preto School of Medicine, a multi-user center that functions up to the standards of modern and traditional facilities in developed countries. The equipment to be acquired will function in a complementary manner. The biophotonic in vivo imaging system has low resolution but high sensitivity for the acquisition and quantification of fluorescence and luminescence in live animals, allowing the luminosity within the organism to be mapped in organs and defined anatomical regions. The multiphoton microscopy system is applied to the acquisition of high-resolution multifluorescence images, obtained over the surface of any organ, capable of revealing details of the extracellular matrix and of subcellular compartments, allowing dynamic events of cell proliferation and cell death to be examined, as well as cell-to-cell interaction, vascularization, innervation, and interactions with pathogens. It also makes it possible to detect intrinsic fluorescence emitted by molecules that function as markers of the metabolic state and oxidative stress, as well as facilitating the study of the dynamics of molecules, organelles, and structural components, through the use of techniques of photoactivation, uncaging, and photoconversion. Together, the two systems are powerful tools for the capture of in vivo images and provide data at complementary levels of resolution, for the evaluation of physiological and pathological events, being applied especially to the study of cancer, stem cells, tissue regeneration, immunology, inflammation, infection, neurobiology, development, aging, and metabolism, as well as to accelerating the process of gene characterization and drug development. (AU)
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