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Internalization and intracellular trafficking of nanoparticles: biological activity and nanotoxicological profile

Grant number: 14/03002-7
Support type:Research Grants - Young Investigators Grants
Duration: March 01, 2015 - February 29, 2020
Field of knowledge:Biological Sciences - Biochemistry
Principal Investigator:Marcelo Bispo de Jesus
Grantee:Marcelo Bispo de Jesus
Home Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
Associated grant(s):15/06134-4 - Multi-User Equipment approved in grant 2014/03002-7: cell imaging multi-mode reader, AP.EMU
Associated scholarship(s):17/10249-7 - Molecular mechanisms triggered by solid lipid nanoparticles in prostate cells PNT2 and PC-3: evaluation of the TGF-Beta pathway, BP.IC
16/16259-1 - Bioinspired gene delivery optimization of nanoparticles in retinal pigmented epithelium model, BP.MS
16/03765-6 - In vitro and in vivo determination of toxicological profile of nanoformulations, BP.PD
15/05026-3 - Internalization and intracellular trafficking of nanoparticles: biological activity and nanotoxicological profile, BP.JP

Abstract

Nanomedicine is presented as a new technology to assist in the treatment and diagnosis of various diseases. However, this new technology brings new challenges, not only in developing new nanodevices, as well as in the interaction of these nanomaterials with biological environment. Understanding the molecular mechanisms involved in the internalization and processing of nanoparticles by eukaryotic cells is vital to develop more efficient and safer nanoparticles. Although the more internalization pathways lead to degradation of internalized material, increasing evidence in the literature suggest that some nanomaterials are biopersistent, thus leading to negative impact on cellular metabolism. Thereby, recently, a new area of knowledge has been established, the nanotoxicology. This project aims to study how the internalization and intracellular trafficking of nanoparticles affects their biological role, e.g. genes delivery, as well as evaluating the adverse effects caused by these nanomaterials at the cellular level. (AU)

Scientific publications (10)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MENDONCA, MONIQUE C. P.; RODRIGUES, NATALIA P.; SCOTT-FORDSMAND, JANECK J.; DE JESUS, MARCELO BISPO; AMORIM, MONICA J. B. The toxicity of silver nanomaterials (NM 300K) is reduced when combined with N-Acetylcysteine: Hazard assessment on Enchytraeus crypticus. Environmental Pollution, v. 256, JAN 2020. Web of Science Citations: 1.
MENDONCA, MONIQUE C. P.; RODRIGUES, NATALIA P.; DE JESUS, MARCELO B.; AMORIM, MONICA J. B. Graphene-Based Nanomaterials in Soil: Ecotoxicity Assessment Using Enchytraeus crypticus Reduced Full Life Cycle. NANOMATERIALS, v. 9, n. 6 JUN 2019. Web of Science Citations: 1.
FACHI, JOSE LUIS; FELIPE, JAQUELINE DE SOUZA; PRAL, LAIS PASSARIELLO; DA SILVA, BRUNA KARADI; CORREA, RENAN OLIVEIRA; PEREIRA DE ANDRADE, MIRELLA CRISTINY; DA FONSECA, DENISE MORAIS; BASSO, PAULO JOSE; SARAIVA CAMARA, NIELS OLSEN; DE SALES E SOUZA, ERICKA LORENNA; MARTINS, FLAVIANO DOS SANTOS; SATO GUIMA, SUZANA EIKO; THOMAS, ANDREW MALTEZ; SETUBAL, JOAO CARLOS; MAGALHAES, YULI THAMIRES; FORTI, FABIO LUIS; CANDREVA, THAMIRIS; RODRIGUES, HOSANA GOMES; DE JESUS, MARCELO BISPO; CONSONNI, SILVIO ROBERTO; FARIAS, ALESSANDRO DOS SANTOS; VARGA-WEISZ, PATRICK; RAMIREZ VINOLO, MARCO AURELIO. Butyrate Protects Mice from Clostridium difficile-Induced Colitis through an HIF-1-Dependent Mechanism. CELL REPORTS, v. 27, n. 3, p. 750+, APR 16 2019. Web of Science Citations: 3.
PADILHA MENDONCA, MONIQUE CULTURATO; FERREIRA, LUIZ BANDEIRA; RIZOLI, CINTIA; BATISTA, ANGELA GIOVANA; MAROSTICA JUNIOR, MARIO ROBERTO; DA SILVA, EMANUELI DO NASCIMENTO; CADORE, SOLANGE; DURAN, NELSON; DA CRUZ-HOFLING, MARIA ALICE; DE JESUS, MARCELO BISPO. N-Acetylcysteine reverses silver nanoparticle intoxication in rats. Nanotoxicology, v. 13, n. 3, p. 326-338, MAR 16 2019. Web of Science Citations: 1.
RADAIC, A.; DE JESUS, M. B. Solid lipid nanoparticles release DNA upon endosomal acidification in human embryonic kidney cells. Nanotechnology, v. 29, n. 31 AUG 3 2018. Web of Science Citations: 1.
BECHER, TIAGO B.; MENDONCA, MONIQUE C. P.; DE FARIAS, MARCELO A.; PORTUGAL, V, RODRIGO; DE JESUS, MARCELO B.; ORNELAS, CATIA. Soft Nanohydrogels Based on Laponite Nanodiscs: A Versatile Drug Delivery Platform for Theranostics and Drug Cocktails. ACS APPLIED MATERIALS & INTERFACES, v. 10, n. 26, p. 21891-21900, JUL 4 2018. Web of Science Citations: 8.
SAMPAIO BONAFE, CARLOS FRANCISCO; CONCEICAO BISPO, JOSE AILTON; DE JESUS, MARCELO BISPO. The Polygonal Model: A Simple Representation of Biomolecules as a Tool for Teaching Metabolism. BIOCHEMISTRY AND MOLECULAR BIOLOGY EDUCATION, v. 46, n. 1, p. 66-75, JAN-FEB 2018. Web of Science Citations: 0.
GUILHERME, VIVIANE A.; RIBEIRO, LIGIA N. M.; TOFOLI, GIOVANA RADOMILLE; FRANZ-MONTAN, MICHELLE; DE PAULA, ENEIDA; DE JESUS, MARCELO BISPO. Current Challenges and Future of Lipid Nanoparticles Formulations for Topical Drug Application to Oral Mucosa, Skin, and Eye. CURRENT PHARMACEUTICAL DESIGN, v. 23, n. 43, p. 6659-6675, 2017. Web of Science Citations: 5.
MENDONCA, MONIQUE C. P.; RIZOLI, CINTIA; AVILA, DAIANA S.; AMORIM, MONICA J. B.; DE JESUS, MARCELO B. Nanomaterials in the Environment: Perspectives on in Vivo Terrestrial Toxicity Testing. FRONTIERS IN ENVIRONMENTAL SCIENCE, v. 5, 2017. Web of Science Citations: 1.
ALLAN RADAIC; GUSTAVO OLIVEIRA PUGLIESE; GUILHERME CAPIRAÇO CAMPESE; FRANCISCO BENEDITO TEIXEIRA PESSINE; MARCELO BISPO DE JESUS. COMO ESTUDAR INTERAÇÕES ENTRE NANOPARTÍCULAS E SISTEMAS BIOLÓGICOS. Química Nova, v. 39, n. 10, p. 1236-1244, Dez. 2016.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.
Filed patent(s) as a result of this research project

Solicitação em análise e dentro do prazo legal de sigilo previsto na legislação PCT/BR2018/000050 - Universidade Estadual de Campinas (UNICAMP) . Solicitação em análise e dentro do prazo legal de sigilo previsto na legislação - August 2018, 24

PROCESSO DE OBTENÇÃO DE NANOPARTÍCULAS BIOSSINTÉTLCAS DE PRATA, NANOPARTÍCULAS BIOSINTÉTLCAS DE PRATA OBTIDAS E SEU USO BR1020170181294 - Universidade Estadual de Campinas (UNICAMP) . Marcelo Bispo de Jesus; Patrick Vianna Garcia; Wagner José Fávaro; Luiz Alberto Bandeira Ferreira; Nelson Eduardo Duran Caballero - August 2017, 24