Identification of phenolic compounds and protective action of Rosmarinus officinallis supplementation on oxidative stress in rats submitted to myocardial infarction

• Grant number: 12/23759-0
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: July 08, 2013
• End date: July 07, 2014
• Field of knowledge: Health Sciences - Nutrition - Nutrition Biochemistry
• Principal Investigator: Sergio Alberto Rupp de Paiva
• Grantee: Bruna Paola Murino Rafacho
• Supervisor: Xiang-Dong Wang
• Host Institution: Faculdade de Medicina (FMB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Institution abroad: Tufts University, United States
• Associated to the scholarship: 11/08956-0 - Influence of diet rosemary supplementation (Rosmarinus officinalis L.) on cardiac remodeling in rats subjected to acute myocardial infarction, BP.DR

Abstract

An increased oxidative status is described after myocardial infarction. On the other hand, many beneficial effects have been attributed to constituents of plant foods, especially antioxidants. Rosemary is one of the most widely used herbs, known for its antioxidant effect. To support its use, it is of great relevance to characterize its compounds and its antioxidant actions. Objectives: identify phenolic compounds present in the rosemary extract used for supplementation in the main project and to determine the antioxidant status after rosemary supplementation in circulation as well as target tissue, cardiac tissue. Methods: rosemary extract produced in Brazil will be tested for antioxidant activity of fat and water soluble components and characterization of phenolic compounds using LC/UV/ESI-MS/MS analysis. Serum and heart tissue collected from animals used on the main project will be analyzed for protein carbonyl determination and total antioxidant performance. Values of the comparison between heart and serum analysis will be presented as mean ± SEM and comparisons will be made by two-way ANOVA test, with significance level of 5%. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PAOLA MURINO RAFACHO, BRUNA; STICE, CAMILLA; LIU, CHUN; GREENBERG, ANDREW; AUSMAN, LYNNE; WANG, XIANG-DONG. Inhibition of diethylnitrosamine-initiated alcohol-promoted hepatic inflammation and precancerous lesions by flavonoid luteolin is associated with increased sirtuin 1 activity in mice. HEPATOBILIARY SURGERY AND NUTRITION, v. 4, n. 2, 1, p. 124-134, APR 2015. (12/23759-0)

PAOLA MURINO RAFACHO, BRUNA; STICE, CAMILLA; LIU, CHUN; GREENBERG, ANDREW; AUSMAN, LYNNE; WANG, XIANG-DONG. Inhibition of diethylnitrosamine-initiated alcohol-promoted hepatic inflammation and precancerous lesions by flavonoid luteolin is associated with increased sirtuin 1 activity in mice. HEPATOBILIARY SURGERY AND NUTRITION, v. 4, n. 2, p. 11-pg., 2015-04-01. (12/23759-0)