In vitro bioaccessibility of murici carotenoids and their esters and influence of phenolic compounds on carotenoid bioaccessibility in mimic food system

• Grant number: 13/23218-1
• Support Opportunities: Scholarships in Brazil - Doctorate
• Effective date (Start): March 01, 2014
• Effective date (End): February 28, 2018
• Field of knowledge: Agronomical Sciences - Food Science and Technology - Food Science
• Principal Investigator: Adriana Zerlotti Mercadante
• Grantee: Daniele Bobrowski Rodrigues
• Host Institution: Faculdade de Engenharia de Alimentos (FEA). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated scholarship(s): 15/15238-8 - Influence of phenolic compounds on in vitro bioaccessibility, uptake and transport of carotenoids by Caco-2 cells, BE.EP.DR

Abstract

Carotenoids and phenolic compounds have important physical-chemical properties related to the prevention and decreased risk of chronic-degenerative diseases. Their action is partially related to the modulation of oxidation reactions by scavenging reactive oxygen (ROS) and nitrogen (RNS) species produced during the oxidative/nitrosative stress. The identification of the compounds formed after the digestion of carotenoids and phenolic compounds and the changes in the antioxidant capacity against reactive species of biological relevance are presented in very few studies. However, this information is essential, since the contents of bioactive compounds in food, as well as the antioxidant capacity, should probably be overestimated when compared to the bioaccessible fraction. In this sense, this project aims to answer the following questions: (i) How do the composition and physical state of systems that mimic food (freshly prepared and freeze-dried emulsions) affect the in vitro bioaccessibility of carotenoids and phenolic compounds? (ii) Does the in vitro digestion have negative or positive impact on the ROS and RSN scavenging capacity of carotenoids and phenolic compounds?; (iii) Which are the bioaccessible compounds available as antioxidants after the in vitro digestion?; (iv) What is the effect of the simultaneous presence of carotenoids and phenolic compounds on the in vitro bioaccessibility and on the antioxidant capacity? In order to answer these questions, different emulsions (food mimetic system) containing standards of carotenoids and phenolic compounds will be prepared. The in vitro digestion (gastric and intestinal phases) will be evaluated by a static method, the ROS and RNS scavenging capacity will be evaluated by specific methods for each reactive species and the compounds formed after the in vitro gastrointestinal digestion will be characterized by high performance liquid chromatograph coupled to a diode array and a mass spectrometer detectors (HPLC-DAD-MSn).

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

RODRIGUES, DANIELE B.; CHITCHUMROONCHOKCHAI, CHUREEPORN; MARIUTTI, LILIAN R. B.; MERCADANTE, ADRIANA Z.; FAILLA, MARK L.. Comparison of Two Static in Vitro Digestion Methods for Screening the Bioaccessibility of Carotenoids in Fruits, Vegetables, and Animal Products. Journal of Agricultural and Food Chemistry, v. 65, n. 51, p. 11220-11228, DEC 27 2017. (13/23218-1, 15/15238-8)

RODRIGUES, DANIELE BOBROWSKI; BARROS MARIUTTI, LILIAN REGINA; MERCADANTE, ADRIANA ZERLOTTI. An in vitro digestion method adapted for carotenoids and carotenoid esters: moving forward towards standardization. FOOD & FUNCTION, v. 7, n. 12, p. 4992-5001, 2016. (12/20988-8, 12/01506-2, 13/23218-1)

MERCADANTE, ADRIANA ZERLOTTI; RODRIGUES, DANIELE B.; PETRY, FABIANE C.; BARROS MARIUTTI, LILIAN REGINA. Carotenoid esters in foods - A review and practical directions on analysis and occurrence. Food Research International, v. 99, n. 2, SI, p. 830-850, SEP 2017. (13/23218-1, 13/07914-8, 13/09804-5)

RODRIGUES, DANIELE BOBROWSKI; MERCADANTE, ADRIANA ZERLOTTI; BARROS MARIUTTI, LILIAN REGINA. Marigold carotenoids: Much more than lutein esters. Food Research International, v. 119, p. 653-664, MAY 2019. (13/23218-1, 15/15238-8)

RODRIGUES, DANIELE BOBROWSKI; BARROS MARIUTTI, LILIAN REGINA; MERCADANTE, ADRIANA ZERLOTTI. Two-step cleanup procedure for the identification of carotenoid esters by liquid chromatography-atmospheric pressure chemical ionization-tandem mass spectrometry. Journal of Chromatography A, v. 1457, p. 116-124, JUL 29 2016. (12/20988-8, 12/01506-2, 13/23218-1)

MERCADANTE, ADRIANA ZERLOTTI; RODRIGUES, DANIELE B.; PETRY, FABIANE C.; BARROS MARIUTTI, LILIAN REGINA. Carotenoid esters in foods - A review and practical directions on analysis and occurrence. Food Research International, v. 99, p. 21-pg., 2017-09-01. (13/23218-1, 13/09804-5, 13/07914-8)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

RODRIGUES, Daniele Bobrowski. Carotenoid and carotenoid esters: analysis and in vitro digestion in individual and co-consumed foods. 2018. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Faculdade de Engenharia de Alimentos Campinas, SP.