Impact of the maternal diet and the intervention with fructooligosaccharide on the human milk microbiota.

• Grant number: 13/26435-3
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: May 01, 2014
• End date: March 16, 2018
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Susana Marta Isay Saad
• Grantee: Marina Padilha
• Host Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 13/07914-8 - FoRC - Food Research Center, AP.CEPID
• Associated scholarship(s): 16/07936-0 - Human milk microbiota analysis by next-generation sequencing: the impact of maternal diet and the intervention with fructooligosaccharides, BE.EP.DR

Abstract

Human milk is recognized as the main component for growth, metabolism, and immune development in infants. Furthermore, during lactation, human milk is an important source of microorganisms for the intestinal colonization of newborns. Mother- related factors have been associated with the human milk microbiota composition. Nevertheless, apparently, there has not been any study in which the maternal diet was evaluated as a modulator of the human milk microbiota. Therefore, the aim of this study is to investigate the impact of the maternal diet on the human milk microbiota composition of healthy women on exclusive breastfeeding, and subsequently, to evaluate the effect of fructooligosaccharides supplementation on the human milk microbiota. In order to investigate a possible association between macronutrients (carbohydrates, lipids, and proteins) and fibers intake with the human milk microbiota, a clinical trial will be divided in two parts. The first part will consist of a cross-sectional study with 150 lactating women, recruited at the University Hospital of University of São Paulo (HU/USP). The second part will consist of a randomized, placebo-controlled clinical trial with 60 lactating from the first part of the clinical trial. The participants will be divided into groups of supplementation and placebo group, which will receive 6 g of fructooligosaccharides + 2 g of maltodextrin or 2 g of maltodextrin, respectively, over a period of 20 days. The dietary assessment will be estimated by the 24 hour recall method (IR24H) and the Quantitative Food Frequency Questionnaire (QFFQ) for use in pregnancy. The microbiota in human milk will be analyzed from milk samples aseptically collected and DNA will be extracted using QIAamp DNA Stool Mini kit. The DNA obtained will be used as template for amplification and sequencing by the Illumina MiSeq® System.

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SILVA VIEIRA, ANTONIO DIOGO; DE SOUZA, CARLOTA BUSSOLO; PADILHA, MARINA; ZOETENDAL, ERWIN GERARD; SMIDT, HAUKE; ISAY SAAD, SUSANA MARTA; VENEMA, KOEN. Impact of a fermented soy beverage supplemented with acerola by-product on the gut microbiota from lean and obese subjects using an in vitro model of the human colon. Applied Microbiology and Biotechnology, v. 105, n. 9, p. 3771-3785, MAY 2021. (13/26435-3, 13/19346-4, 13/50506-8, 16/07940-7)

PADILHA, MARINA; BREJNROD, ASKER; DANNESKIOLD-SAMSOE, NIELS BANHOS; HOFFMANN, CHRISTIAN; IAUCCI, JULIA DE MELO; CABRAL, VANESSA PEREIRA; XAVIER-SANTOS, DOUGLAS; TADDEI, CARLA ROMANO; KRISTIANSEN, KARSTEN; ISAY SAAD, SUSANA MARTA. Response of the Human Milk Microbiota to a Maternal Prebiotic Intervention Is Individual and Influenced by Maternal Age. NUTRIENTS, v. 12, n. 4, APR 2020. (13/07914-8, 13/26435-3, 16/07936-0)

PADILHA, M.; IAUCCI, J. M.; CABRAL, V. P.; DINIZ, E. M. A.; TADDEI, C. R.; SAAD, I, S. M.. Maternal antibiotic prophylaxis affects Bifidobacterium spp. counts in the human milk, during the first week after delivery. BENEFICIAL MICROBES, v. 10, n. 2, p. 155-163, 2019. (16/07936-0, 13/26435-3, 13/07914-8)

PADILHA, MARINA; DANNESKIOLD-SAMSOE, NIELS BANHOS; BREJNROD, ASKER; HOFFMANN, CHRISTIAN; CABRAL, VANESSA PEREIRA; IAUCCI, JULIA DE MELO; SALES, CRISTIANE HERMES; FISBERG, REGINA MARA; CORTEZ, RAMON VITOR; BRIX, SUSANNE; et al. The Human Milk Microbiota is Modulated by Maternal Diet. MICROORGANISMS, v. 7, n. 11, NOV 2019. (16/07936-0, 13/26435-3, 13/07914-8)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

PADILHA, Marina. Impact of the maternal diet and the intervention with fructooligosaccharide on thehuman milk microbiota. 2018. Doctoral Thesis - Universidade de São Paulo (USP). Conjunto das Químicas (IQ e FCF) (CQ/DBDCQ) São Paulo.