Nuclear proteome alterations induced by growth factor along cell cycle in mice tumour cells

• Grant number: 15/04867-4
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Effective date (Start): April 01, 2015
• Effective date (End): March 31, 2017
• Field of knowledge: Biological Sciences - Biochemistry
• Principal Investigator: Julia Pinheiro Chagas da Cunha
• Grantee: Mariana de Camargo Lopes
• Host Institution: Instituto Butantan. Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil
• Associated research grant: 11/22619-7 - Nuclei and chromatin alterations through cell cycle and senescence in mammalian cells, AP.JP

Abstract

Some nuclear proteins are essential for the maintenance and regulation of the genome, transforming extracellular and intracellular signals into changes in gene expression and phenotype. In this project, we aim to analyze the nuclear proteome at different phases of the cell cycle and senescence of mouse tumor cells stimulated with FGF2. To this end, nuclear extracts will be systematically analyzed by mass spectrometry, allowing the detailed investigation of proteins closely related to the management of gene expression, cell phenotype, cell replication, chromatin remodeling and DNA repair and damage. Quantitative analysis will be performed using Spike-in SILAC and/or label free quantification. Using an integrative bioinformatics analysis, we believe will be possible to identify key molecules responsible for the induction and maintenance cell cycle arrest and senescence induced by FGF2, allowing a better understanding of cell cycle and tumor biology. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

LUND, PEDER J.; LOPES, MARIANA; SIDOLI, SIMONE; CORADIN, MARIEL; DE LUNA VITORINO, FRANCISCA NATHALIA; CHAGAS DA CUNHA, JULIA PINHEIRO; GARCIA, BENJAMIN A.. FGF-2 induces a failure of cell cycle progression in cells harboring amplified K-Ras, revealing new insights into oncogene-induced senescence. MOLECULAR OMICS, v. 17, n. 5, p. 725-739, OCT 11 2021. (16/24881-4, 15/04867-4, 13/07467-1, 17/15835-1, 18/15553-9, 11/22619-7, 17/18344-9)

LEANDRO DE JESUS, TERESA CRISTINA; NUNES, VINICIUS SANTANA; LOPES, MARIANA DE CAMARGO; MARTIL, DAIANA EVELIN; IWAI, LEO KEI; MORETTI, NILMAR SILVIO; MACHADO, FABRICIO CASTRO; DE LIMA-STEIN, MARIANA L.; THIEMANN, OTAVIO HENRIQUE; ELIAS, MARIA CAROLINA; et al. Chromatin Proteomics Reveals Variable Histone Modifications during the Life Cycle of Trypanosoma cruzi. JOURNAL OF PROTEOME RESEARCH, v. 15, n. 6, p. 2039-2051, JUN 2016. (13/07467-1, 14/03714-7, 08/57910-0, 15/04867-4, 11/06087-5, 11/51973-3, 11/22619-7, 14/01577-2)

LUND, PEDER J.; LOPES, MARIANA; SIDOLI, SIMONE; CORADIN, MARIEL; DE LUNA VITORINO, FRANCISCA NATHALIA; CHAGAS DA CUNHA, JULIA PINHEIRO; GARCIA, BENJAMIN A.. FGF-2 induces a failure of cell cycle progression in cells harboring amplified K-Ras, revealing new insights into oncogene-induced senescence. MOLECULAR OMICS, JUN 2021. (11/22619-7, 16/24881-4, 17/15835-1, 18/15553-9, 15/04867-4, 17/18344-9, 13/07467-1)