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Nuclear proteome alterations induced by growth factor along cell cycle in mice tumour cells

Grant number: 15/04867-4
Support type:Scholarships in Brazil - Doctorate (Direct)
Effective date (Start): April 01, 2015
Effective date (End): March 31, 2017
Field of knowledge:Biological Sciences - Biochemistry
Principal Investigator:Julia Pinheiro Chagas da Cunha
Grantee:Mariana de Camargo Lopes
Home Institution: Instituto Butantan. Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil
Associated research grant:11/22619-7 - Nuclei and chromatin alterations through cell cycle and senescence in mammalian cells, AP.JP

Abstract

Some nuclear proteins are essential for the maintenance and regulation of the genome, transforming extracellular and intracellular signals into changes in gene expression and phenotype. In this project, we aim to analyze the nuclear proteome at different phases of the cell cycle and senescence of mouse tumor cells stimulated with FGF2. To this end, nuclear extracts will be systematically analyzed by mass spectrometry, allowing the detailed investigation of proteins closely related to the management of gene expression, cell phenotype, cell replication, chromatin remodeling and DNA repair and damage. Quantitative analysis will be performed using Spike-in SILAC and/or label free quantification. Using an integrative bioinformatics analysis, we believe will be possible to identify key molecules responsible for the induction and maintenance cell cycle arrest and senescence induced by FGF2, allowing a better understanding of cell cycle and tumor biology. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
LEANDRO DE JESUS, TERESA CRISTINA; NUNES, VINICIUS SANTANA; LOPES, MARIANA DE CAMARGO; MARTIL, DAIANA EVELIN; IWAI, LEO KEI; MORETTI, NILMAR SILVIO; MACHADO, FABRICIO CASTRO; DE LIMA-STEIN, MARIANA L.; THIEMANN, OTAVIO HENRIQUE; ELIAS, MARIA CAROLINA; JANZEN, CHRISTIAN; SCHENKMAN, SERGIO; CHAGAS DA CUNHA, JULIA PINHEIRO. Chromatin Proteomics Reveals Variable Histone Modifications during the Life Cycle of Trypanosoma cruzi. JOURNAL OF PROTEOME RESEARCH, v. 15, n. 6, p. 2039-2051, JUN 2016. Web of Science Citations: 12.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.