Comparative genome analysis of Streptococcus suis strains isolated from swine in Brazil

• Grant number: 15/26159-1
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: May 01, 2016
• End date: September 30, 2019
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
• Principal Investigator: Andrea Micke Moreno
• Grantee: Carlos Emilio Cabrera Matajira
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Streptococcus suis is an important pathogen in swine production systems in the world. Clinical signs of infection for this agent are variable and can be observed meningitis, arthritis, polyserositis, pneumonia, urinary tract infection and abortion. In recent years, with the growing of pigs intensive production systems, the occurrence of outbreaks of infection by S. suis increased. The high number of carriers of S. suis in animals at slaughter also increases the risk of infection to humans involved in the production chain, as well as to the people handling the meat "in natura". The infection in humans has been described in several countries as a cause of meningitis, pneumonia, endocarditis, sepsis and even death. This project aims the characterization of 215 isolates of S. suis that caused outbreaks in pig farms from different states of the country. This characterization will include the identification of the most prevalent serotypes, the determination of the resistance profile and the changes in this in the last 12 years, evaluation of the strains by PFGE and sequencing of the genome of a group of selected strains. From the sequencing of genomes will be determined the type sequences (STs) that are taking place in the country, genes conferring resistance to antibiotics and newly described virulence genes. These data will serve as a basis for future studies in the country involving the control of the disease in swine and even research involving the occurrence of this agent in humans or other animal species.

Scientific publications

(20)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FRANCO F. CALDERARO; LUISA Z. MORENO; DANIELA S. DOTO; CARLOS E.C. MATAJIRA; VASCO T.M. GOMES; THAIS S.P. FERREIRA; RENAN E. MESQUITA; ANDREA M. MORENO. Caracterização de Streptococcus suis por sorotipagem, SE-AFLP e perfil de virulência. Pesquisa Veterinária Brasileira, v. 36, n. 8, p. 701-704, 2016-08-00. (15/26159-1)

MATAJIRA, CARLOS E. C.; MORENO, LUISA Z.; GOMES, VASCO T. M.; SILVA, ANA PAULA S.; MESQUITA, RENAN E.; DOTO, DANIELA S.; CALDERARO, FRANCO F.; DE SOUZA, FERNANDO N.; CHRIST, ANA PAULA G.; SATO, MARIA INES Z.; et al. Evaluation of protein spectra cluster analysis for Streptococcus spp. identification from various swine clinical samples. JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION, v. 29, n. 2, p. 245-249, MAR 2017. (15/26159-1, 13/17136-2, 13/16946-0)

MATAJIRA, CARLOS E. C.; MORENO, LUISA Z.; POOR, ANDRE P.; GOMES, VASCO T. M.; DALMUTT, ANDRESSA C.; PARRA, BEATRIZ M.; DE OLIVEIRA, CAROLINA H.; BARBOSA, MIKAELA R. F.; SATO, MARIA INES Z.; CALDERARO, FRANCO F.; et al. Streptococcus suis in Brazil: Genotypic, Virulence, and Resistance Profiling of Strains Isolated from Pigs between 2001 and 2016. PATHOGENS, v. 9, n. 1, JAN 2020. (17/09515-4, 15/26159-1, 16/25745-7, 19/01192-7)

MORENO, LUISA Z.; MATAJIRA, CARLOS E. C.; POOR, ANDRE P.; MESQUITA, RENAN E.; GOMES, VASCO T. M.; SILVA, ANA PAULA S.; AMIGO, CRISTINA R.; CHRIST, ANA PAULA G.; BARBOSA, MIKAELA R. F.; SATO, MARIA INES Z.; et al. Identification through MALDI-TOF mass spectrometry and antimicrobial susceptibility profiling of bacterial pathogens isolated from sow urinary tract infection. VETERINARY QUARTERLY, v. 38, n. 1, p. 1-8, DEC 7 2017. (15/26159-1, 16/25745-7)

ABRAHAO, CARLOS R.; MORENO, LUISA Z.; SILVA, JEAN C. R.; BENITES, NILSON R.; MATAJIRA, CARLOS E. C.; FERREIRA, FERNANDO; MORENO, ANDREA M.; DIAS, RICARDO A.. Salmonella enterica in Invasive Lizard from Fernando de Noronha Archipelago: Serotyping, Antimicrobial Resistance and Molecular Epidemiology. MICROORGANISMS, v. 8, n. 12, DEC 2020. (15/26159-1, 16/25745-7)

MORENO, ANDREA MICKE; MORENO, LUISA ZANOLLI; POOR, ANDRE PEGORARO; MATAJIRA, CARLOS EMILIO CABRERA; MORENO, MARINA; DE MOURA GOMES, VASCO TULIO; RIBEIRO DA SILVA, GIVAGO FARIA; TAKEUTI, KARINE LUDWIG; BARCELLOS, DAVID EMILIO. Antimicrobial Resistance Profile of Staphylococcus hyicus Strains Isolated from Brazilian Swine Herds. ANTIBIOTICS-BASEL, v. 11, n. 2, p. 12-pg., 2022-02-01. (11/08541-5, 13/17136-2, 15/26159-1, 13/16946-0)

CALDERARO, FRANCO F.; MORENO, LUISA Z.; DOTO, DANIELA S.; MATAJIRA, CARLOS E. C.; GOMES, VASCO T. M.; FERREIRA, THAIS S. P.; MESQUITA, RENAN E.; MORENO, ANDREA M.. Characterization of Streptococcus suis through serotyping, SE-AFLP and virulence profile. Pesquisa Veterinária Brasileira, v. 36, n. 8, p. 4-pg., 2016-08-01. (15/26159-1)

MONTEIRO, MATHEUS SALIBA; MATIAS, DEBORA NOVAIS; POOR, ANDRE PEGORARO; DUTRA, MAURICIO CABRAL; MORENO, LUISA ZANOLLI; PARRA, BEATRIZ MARTINS; SILVA, ANA PAULA SANTOS; CABRERA MATAJIRA, CARLOS EMILIO; DE MOURA GOMES, VASCO TULIO; FUNADA BARBOSA, MIKAELA RENATA; et al. Causes of Sow Mortality and Risks to Post-Mortem Findings in a Brazilian Intensive Swine Production System. ANIMALS, v. 12, n. 14, p. 18-pg., 2022-07-01. (19/01192-7, 19/17683-0, 15/26159-1, 16/25745-7)

POOR, ANDRE P.; MORENO, LUISA Z.; MATAJIRA, CARLOS E. C.; PARRA, BEATRIZ M.; GOMES, VASCO T. M.; SILVA, ANA PAULA S.; DUTRA, MAURICIO C.; CHRIST, ANA PAULA G.; BARBOSA, MIKAELA R. F.; SATO, MARIA INES Z.; et al. Characterization of Corynebacterium diphtheriae, C. confusum and C-amycolatum isolated from sows with genitourinary infection. Veterinary Microbiology, v. 207, p. 149-152, AUG 2017. (15/26159-1, 15/26412-9, 13/17136-2, 13/16946-0)

MORENO, LUISA Z.; MATAJIRA, CARLOS E. C.; GOMES, VASCO T. M.; SILVA, ANA PAULA S.; MESQUITA, RENAN E.; CHRIST, ANA PAULA G.; SATO, MARIA INES Z.; MORENO, ANDREA M.. Molecular and antimicrobial susceptibility profiling of atypical Streptococcus species from porcine clinical specimens. INFECTION GENETICS AND EVOLUTION, v. 44, p. 376-381, OCT 2016. (15/26159-1, 13/17136-2, 13/16946-0)

LUISA Z MORENO; MAURICIO C DUTRA; MARINA MORENO; THAIS SP FERREIRA; GIVAGO FR DA SILVA; CARLOS EC MATAJIRA; ANA PAULA S SILVA; ANDREA M MORENO. Vancomycin-intermediate livestock-associated methicillin-resistant Staphylococcus aureus ST398/t9538 from swine in Brazil. Memórias do Instituto Oswaldo Cruz, v. 111, n. 10, p. 659-661, 2016-10-00. (15/26159-1, 11/08541-5, 13/17136-2)

MORENO, LUISA Z.; DA COSTA, BARBARA L. P.; MATAJIRA, CARLOS E. C.; GOMES, VASCO T. M.; MESQUITA, RENAN E.; SILVA, ANA PAULA S.; MORENO, ANDREA M.. Molecular and antimicrobial susceptibility profiling of Streptococcus dysgalactiae isolated from swine. DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, v. 86, n. 2, p. 178-180, OCT 2016. (15/26159-1, 13/17136-2, 13/16946-0)

MORENO, LUISA Z.; MATAJIRA, CARLOS E. C.; DA COSTA, BARBARA L. P.; FERREIRA, THAIS S. P.; SILVA, GIVAGO F. R.; DUTRA, MAURICIO C.; GOMES, VASCO T. M.; SILVA, ANA PAULA S.; CHRIST, ANA PAULA G.; SATO, MARIA INES Z.; et al. Characterization of porcine Trueperella pyogenes by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), molecular typing and antimicrobial susceptibility profiling in Sao Paulo State. COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES, v. 51, p. 49-53, APR 2017. (15/26159-1, 13/17136-2, 13/16946-0)

DE OLIVEIRA, MIRELA C. V.; CAMARGO, BEATRIZ Q.; CUNHA, MARCOS P. V.; SAIDENBERG, ANDRE BECKER; TEIXEIRA, RODRIGO H. F.; MATAJIRA, CARLOS E. C.; MORENO, LUISA Z.; GOMES, VASCO T. M.; CHRIST, ANA P. G.; BARBOSA, MIKAELA R. F.; et al. Free-Ranging Synanthropic Birds (Ardea alba and Columba livia domestica) as Carriers of Salmonella spp. and Diarrheagenic Escherichia coli in the Vicinity of an Urban Zoo. VECTOR-BORNE AND ZOONOTIC DISEASES, v. 18, n. 1, p. 65-69, JAN 2018. (14/11523-7, 15/26159-1, 16/12641-9)

MORENO, LUISA Z.; DUTRA, MAURICIO C.; MORENO, MARINA; FERREIRA, THAIS S. P.; DA SILVA, GIVAGO F. R.; MATAJIRA, CARLOS E. C.; SILVA, ANA PAULA S.; MORENO, ANDREA M.. Vancomycin-intermediate livestock-associated methicillin-resistant Staphylococcus aureus ST398/t9538 from swine in Brazil. Memórias do Instituto Oswaldo Cruz, v. 111, n. 10, p. 3-pg., 2016-10-01. (15/26159-1, 11/08541-5, 13/17136-2)

POOR, ANDRE P.; MORENO, LUISA Z.; MONTEIRO, MATHEUS S.; MATAJIRA, CARLOS E. C.; DUTRA, MAURICIO C.; LEAL, DIEGO F.; SILVA, ANA PAULA S.; GOMES, VASCO T. M.; BARBOSA, MIKAELA R. F.; SATO, MARIA INES Z.; et al. Vaginal microbiota signatures in healthy and purulent vulvar discharge sows. SCIENTIFIC REPORTS, v. 12, n. 1, p. 12-pg., 2022-06-01. (19/17683-0, 16/25745-7, 19/01192-7, 15/26159-1)

MATAJIRA, CARLOS E. C.; MORENO, LUISA Z.; POOR, ANDRE P.; MONTEIRO, MATHEUS S.; GOMES, VASCO T. D. M.; BARBOSA, MIKAELA R. F.; SATO, MARIA I. Z.; MORENO, ANDREA M.. Identification, genotyping, and genomic comparison of Streptococcus porcinus isolated from diseased swine in Brazil. JOURNAL OF INFECTION IN DEVELOPING COUNTRIES, v. 18, n. 10, p. 9-pg., 2024-10-01. (17/09515-4, 15/26159-1, 19/17683-0, 16/25745-7, 19/01192-7)

MATAJIRA, CARLOS E. C.; MORENO, LUISA Z.; GOMES, VASCO T. M.; SILVA, ANA PAULA S.; MESQUITA, RENAN E.; AMIGO, CRISTINA R.; CHRIST, ANA PAULA G.; SATO, MARIA INES Z.; MORENO, ANDREA M.. Molecular and antimicrobial susceptibility characterization of Globicatella sulfidifaciens isolated from sow's urinary tract infection. VETERINARY QUARTERLY, v. 37, n. 1, p. 48-51, 2017. (13/17136-2, 13/16946-0, 15/26159-1)

XAVIER OLIVEIRA, MARIA GABRIELA; NOGUEIRA PRESSINOTTI, LEANDRO; SPINOLA CARVALHO, GIOVANE; VILELA OLIVEIRA, MIRELA CAROLINE; MORENO, LUISA ZANOLLI; CABRERA MATAJIRA, CARLOS EMILIO; SPINOLA BERGAMO, ALESSANDRO; MANUEL ALEIXO, VICTOR; CAIXETA VEIGA, ALEXANDRE; DE SOUZA CORSINO, ELVIS; et al. Arcobacter spp. in fecal samples from Brazilian farmed caimans (Caiman yacare, Daudin 1802). TROPICAL ANIMAL HEALTH AND PRODUCTION, v. 49, n. 4, p. 777-782, APR 2017. (13/17136-2, 14/07837-6, 14/06584-7, 15/26159-1)

CABRERA MATAJIRA, CARLOS EMILIO; POOR, ANDRE PEGORARO; MORENO, LUISA ZANOLLI; MONTEIRO, MATHEUS SALIBA; DALMUTT, ANDRESSA CARINE; MOURA GOMES, VASCO TULIO; DUTRA, MAURICIO CABRAL; FUNADA BARBOSA, MIKAELA RENATA; ZANOLLI SATO, MARIA INES; MORENO, ANDREA MICKE. Vagococcus sp. a porcine pathogen: molecular and phenotypic characterization of strains isolated from diseased pigs in Brazil. JOURNAL OF INFECTION IN DEVELOPING COUNTRIES, v. 14, n. 11, p. 6-pg., 2020-11-01. (17/09515-4, 15/26159-1)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

MATAJIRA, Carlos Emilio Cabrera. Comparative genome analysis of Streptococcus suis strains isolated from swine in Brazil. 2020. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia (FMVZ/SBD) São Paulo.