The role of a nonribosomal peptide in promoting swarming in Pseudovibrio brasiliensis Ab134 and related bacteria

• Grant number: 18/10742-8
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: August 01, 2018
• End date: July 31, 2019
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Organic Chemistry
• Principal Investigator: Roberto Gomes de Souza Berlinck
• Grantee: Laura Pavan Ióca
• Supervisor: Alessandra S. Eustaquio
• Host Institution: Instituto de Química de São Carlos (IQSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
• Institution abroad: University of Illinois at Chicago (UIC), United States
• Associated to the scholarship: 16/05133-7 - Study of marine-derived microrganisms metabolism: chemical profile and biosynthesis, BP.DR

Abstract

Genome analyses of the alpha-proteobacterium Pseudovibrio brasiliensis Ab134, a symbiont of the sponge Arenosclera brasiliensis, showed the presence of at least six biosynthetic gene clusters (BGCs) encoding the biosynthesis of secondary metabolites. Among these, a nonribosomal peptide synthetase (NRPS) BCG was shown to be involved in the swarming capability of this bacterial strain. The NRPS BGC is also found in Pseudomonas species, which belong to the class of gamma-proteobacteria, and which are known pathogens of plants and animals. The ability to swarm may facilitate symbiotic or pathogenic associations with host tissues. However, detailed knowledge about the mechanisms of this motility behavior is lacking. This project aims to study the role and mechanism of the nonribosomal peptide in promoting swarming by Pseudovibrio and Pseudomonas species, expanding our knowledge about the conserved mechanisms that proteobacteria employ to coordinate swarming and to colonize eukaryotic hosts.

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

KAHLERT, LUKAS; BERNARDI, DARLON; HAUSER, MAURICE; IOCA, LAURA P.; BERLINCK, ROBERTO G. S.; SKELLAM, ELIZABETH J.; COX, RUSSELL J.. Early Oxidative Transformations During the Biosynthesis of Terrein and Related Natural Products. CHEMISTRY-A EUROPEAN JOURNAL, v. 27, n. 46, p. 11895-11903, AUG 16 2021. (16/05133-7, 18/10742-8, 16/21341-9, 13/50228-8, 20/11691-8, 19/17721-9)

BERLINCK, ROBERTO G. S.; CRNKOVIC, CAMILA M.; GUBIANI, JULIANA R.; BERNARDI, DARLON I.; IOCA, LAURA P.; QUINTANA-BULLA, JAIRO I.. The isolation of water-soluble natural products - challenges, strategies and perspectives. NATURAL PRODUCT REPORTS, OCT 2021. (13/50228-8, 16/16033-3, 19/00447-1, 20/11691-8, 16/05133-7, 17/06014-4, 16/21341-9, 18/10742-8)

KAHLERT, LUKAS; BERNARDI, DARLON; HAUSER, MAURICE; IOCA, LAURA P.; BERLINCK, ROBERTO G. S.; SKELLAM, ELIZABETH J.; COX, RUSSELL J.. Early Oxidative Transformations During the Biosynthesis of Terrein and Related Natural Products. CHEMISTRY-A EUROPEAN JOURNAL, JUN 2021. (19/17721-9, 18/10742-8, 16/05133-7, 16/21341-9, 13/50228-8, 20/11691-8)

IOCA, LAURA P.; DAI, YITAO; KUNAKOM, SYLVIA; DIAZ-ESPINOSA, JENNIFER; KRUNIC, ALEKSEJ; CRNKOVIC, CAMILA M.; ORJALA, JIMMY; SANCHEZ, LAURA M.; FERREIRA, ANTONIO G.; BERLINCK, ROBERTO G. S.; et al. A Family of Nonribosomal Peptides Modulate Collective Behavior in Pseudovibrio Bacteria Isolated from Marine Sponges{*}{*}. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, v. 60, n. 29, p. 15891-15898, JUL 12 2021. (15/01017-0, 16/05133-7, 18/10742-8)