Extraction and separation of phenolic compounds from green propolis by a bidimensional system

• Grant number: 20/03623-2
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: September 01, 2020
• End date: April 30, 2025
• Field of knowledge: Agronomical Sciences - Food Science and Technology - Food Engineering
• Principal Investigator: Maurício Ariel Rostagno
• Grantee: Letícia Sanches Contieri
• Host Institution: Faculdade de Ciências Aplicadas (FCA). Universidade Estadual de Campinas (UNICAMP). Limeira , SP, Brazil
• Associated research grant: 18/14582-5 - Development of a two-dimensional extraction, separation and analysis system for evaluating the bioactivity of phytochemicals, AP.JP2
• Associated scholarship(s): 21/11023-8 - Development of sustainable methods for propolis extracts by the application of non-volatile alternative solvents, BE.EP.DD

Abstract

Green propolis has high biological potential due to the presence of bioactive compounds. Green propolis has the highest selling price in the international market, suggesting that there is a relationship between its color and its chemical composition, rich in phenolics acids. Advanced extraction techniques should be used to make better use of these compounds. This research project proposes the development of a two-dimensional extraction, separation, and analysis system (PLEgrad-SPE x UHPLC) by coupling extraction with pressurized liquids in gradient (PLEgrad) with Solid-Phase Extraction (SPE) in the first dimension and orthogonally with Ultra-High-Pressure Chromatography (UHPLC) in the second dimension. The objective is to develop a system that allows the characterization of the chemical composition of natural raw materials in a fast, efficient and real-time monitoring while allowing the production of small quantities of extracts obtained through the fractionation of the present compounds. These fractions should be well characterized chemically and with low contamination between them. Initially, rapid UHPLC analysis methods will be developed that will be used in the second separation dimension. Several analysis conditions (temperature, column size, flow, volume, and injection solvent and mobile phase composition) will be evaluated for an ultra-fast separation method (time between samples < 2.0 min). Subsequently, different extraction conditions will be evaluated by SPE coupled gradient PLE, including temperature, pressure, sample amount, adsorbent type, flow, solvent, and duration of the extraction/elution gradiente. (AU)

Scientific publications

(18)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

VIGANO, JULIANE; STRIEDER, MONIQUE MARTINS; PIZANI, RODRIGO S.; CONTIERI, LETICIA S.; MESQUITA, LEONARDO M. DE SOUZA; ROSTAGNO, MAURICIO A.. Application of an integrative system (2D PLExHPLC-PDA) for bioactive compound extraction and online quantification: Advantages, validation, and considerations. Analytica Chimica Acta, v. 1272, p. 9-pg., 2023-06-15. (18/14582-5, 20/08421-9, 20/15774-5, 19/13496-0, 21/12264-9, 20/03623-2, 20/04067-6)

DE SOUZA MESQUITA, LEONARDO M.; CONTIERI, LETICIA S.; SOSA, FILIPE H. B.; PIZANI, RODRIGO S.; CHAVES, JAISA; VIGANO, JULIANE; VENTURA, SONIA P. M.; ROSTAGNO, MAURICIO A.. Combining eutectic solvents and pressurized liquid extraction coupled in-line with solid-phase extraction to recover, purify and stabilize anthocyanins from Brazilian berry waste. GREEN CHEMISTRY, v. 25, n. 5, p. 14-pg., 2023-02-21. (21/11022-1, 18/14582-5, 20/08421-9, 20/15774-5, 19/13496-0, 20/03623-2, 21/11023-8, 20/04067-6)

SANCHES, VITOR L.; DE SOUZA MESQUITA, LEONARDO M.; VIGANO, JULIANE; CONTIERI, LETICIA S.; PIZANI, RODRIGO; CHAVES, JAISA; DA SILVA, LAISE CAPELASSO; DE SOUZA, MARIANA CORREA; BREITKREITZ, MARCIA CRISTINA; ROSTAGNO, MAURICIO A.. Insights on the Extraction and Analysis of Phenolic Compounds from Citrus Fruits: Green Perspectives and Current Status. CRITICAL REVIEWS IN ANALYTICAL CHEMISTRY, v. N/A, p. 27-pg., 2022-08-16. (18/14582-5, 20/15774-5, 19/13496-0, 19/24537-0, 20/08421-9, 20/03623-2)

MESQUITA, LEONARDO M. DE SOUZA; CONTIERI, LETICIA S.; SANCHES, VITOR L.; KAMIKAWACHI, RENAN; SOSA, FILIPE H. B.; VILEGAS, WAGNER; ROSTAGNO, MAURICIO A.. Fast and green universal method to analyze and quantify anthocyanins in natural products by UPLC-PDA. Food Chemistry, v. 428, p. 11-pg., 2023-12-01. (21/11022-1, 18/14582-5, 20/08421-9, 19/13496-0, 20/03623-2, 21/11023-8)

SANCHES, VITOR L.; STRIEDER, MONIQUE MARTINS; BREITKREITZ, MARCIA CRISTINA; BEZERRA, ROSANGELA MARIA NEVES; CHAVES, JAISA O.; DE OLIVEIRA, ISADORA LOPES; MESQUITA, LEONARDO M. DE SOUZA; VIGANO, JULIANE; CONTIERI, LETICIA SANCHES; PIZANI, RODRIGO STEIN; et al. Pressurized liquid extraction assisted by high-intensity ultrasound to obtain hesperidin from lime waste: Integration of in-line purification and on-line chromatographic analysis. Food Research International, v. 182, p. 9-pg., 2024-02-28. (20/08421-9, 19/24537-0, 20/03623-2, 19/13496-0, 21/13638-0, 18/14582-5)

MESQUITA, LEONARDO M. DE SOUZA; VIGANO, JULIANE; VEGGI, PRISCILLA; CONTIERI, LETICIA S.; SOSA, FILIPE H. B.; DE ROSSO, VERIDIANA VERA; VENTURA, SONIA P. M.; ROSTAGNO, MAURICIO A.. Techno-Economic analysis of an efficient anthocyanin extraction process from grape pomace using eutectic solvents ― A critical panorama regarding drying techniques and reusability of solvents. Separation and Purification Technology, v. 347, p. 9-pg., 2024-04-26. (20/15774-5, 21/11022-1, 20/08421-9, 20/03623-2, 19/13496-0, 16/18910-1, 21/11023-8, 18/14582-5)

MESQUITA, LEONARDO M. DE SOUZA; CONTIERI, LETICIA S.; A. E SILVA, FRANCISCA; BAGINI, RAFAEL HENRIQUE; BRAGAGNOLO, FELIPE S.; STRIEDER, MONIQUE M.; SOSA, FILIPE H. B.; SCHAEFFER, NICOLAS; FREIRE, MARA G.; VENTURA, SONIA P. M.; et al. Path2Green: introducing 12 green extraction principles and a novel metric for assessing sustainability in biomass valorization. GREEN CHEMISTRY, v. 26, n. 19, p. 21-pg., 2024-08-12. (20/15774-5, 21/11022-1, 15/00658-1, 20/08421-9, 13/04304-4, 23/12621-1, 22/10469-5, 20/03623-2, 19/13496-0, 21/11023-8, 21/12264-9, 18/14582-5, 23/05722-6)

DA SILVA, LAISE C.; VIGANO, JULIANE; DE SOUZA MESQUITA, LEONARDO M.; BAIAO DIAS, ARTHUR L.; DE SOUZA, MARIANA C.; SANCHES, VITOR L.; CHAVES, JAISA O.; PIZANI, RODRIGO S.; CONTIERI, LETICIA S.; ROSTAGNO, MAURICIO A.. Recent advances and trends in extraction techniques to recover polyphenols compounds from apple by-products. FOOD CHEMISTRY-X, v. 12, DEC 30 2021. (20/08421-9, 20/03623-2, 19/24537-0, 20/15774-5, 19/18772-6, 19/13496-0, 18/17089-8, 20/04067-6, 18/14582-5)

CONTIERI, LETICIA S.; MESQUITA, LEONARDO M. DE SOUZA; SANCHES, VITOR L.; CHAVES, JAISA; PIZANI, RODRIGO S.; DA SILVA, LAISE C.; VIGANO, JULIANE; VENTURA, SONIA P. M.; ROSTAGNO, MAURICIO A.. Recent progress on the recovery of bioactive compounds obtained from propolis as a natural resource: Processes, and applications. Separation and Purification Technology, v. 298, p. 18-pg., 2022-07-06. (18/17089-8, 19/18772-6, 18/14582-5, 20/08421-9, 19/24537-0, 20/15774-5, 20/03623-2, 20/04067-6)

PIZANI, RODRIGO S.; VIGANO, JULIANE; DE SOUZA MESQUITA, LEONARDO M.; CONTIERI, LETICIA S.; SANCHES, VITOR L.; CHAVES, JAISA O.; SOUZA, MARIANA C.; DA SILVA, LAISE C.; ROSTAGNO, MAURICIO A.. Beyond aroma: A review on advanced extraction processes from rosemary (Rosmarinus officinalis) and sage (Salvia officinalis) to produce phenolic acids and diterpenes. TRENDS IN FOOD SCIENCE & TECHNOLOGY, v. 127, p. 18-pg., 2022-08-02. (18/17089-8, 18/14582-5, 20/08421-9, 19/24537-0, 20/15774-5, 20/03623-2, 19/13496-0, 20/04067-6)

KHOLANY, MARIAM; MACARIO, INES P. E.; VELOSO, TELMA; CONTIERI, LETICIA S.; VAZ, BARBARA M. C.; PEREIRA, JOANA L.; NUNES, CLAUDIA; COUTINHO, JOAO A. P.; ROSTAGNO, MAURICIO A.; VENTURA, SONIA P. M.; et al. Bio-based solvents as a sustainable alternative to develop a multiproduct biorefinery process from archaebacteria Halobacterium salinarum R1. GREEN CHEMISTRY, v. 26, n. 5, p. 14-pg., 2024-01-30. (21/11022-1, 20/08421-9, 20/03623-2, 19/13496-0, 21/11023-8, 18/14582-5)

CONTIERI, LETICIA SANCHES; MESQUITA, LEONARDO MENDES DE SOUZA; FERREIRA, VANESSA COSME; MORENO, JUVER ANDREY JIMENEZ; PIZANI, RODRIGO STEIN; CARNEIRO, TANIA FORSTER; ROSTAGNO, MAURICIO ARIEL. Sustainable and Innovative Method for Real-Time Extraction and Analysis of Polyphenols from Green Propolis by Pressurized Liquid Extraction Coupled Inline with Photodiode Array Detection (PLE-PDA). ACS SUSTAINABLE CHEMISTRY & ENGINEERING, v. 12, n. 52, p. 13-pg., 2024-12-18. (20/03623-2, 18/14582-5, 20/08421-9, 23/04479-0, 19/13496-0)

PIZANI, RODRIGO S.; VIGANO, JULIANE; CONTIERI, LETICIA S.; STRIEDER, MONIQUE M.; KAMIKAWASHI, RENAN K.; VILEGAS, WAGNER; MESQUITA, LEONARDO M. DE SOUZA; ROSTAGNO, MAURICIO A.. New selective and sustainable ultrasound-assisted extraction procedure to recover carnosic and rosmarinic acids from Rosmarinus officinalis by sequential use of bio-based solvents. Food Chemistry, v. 435, p. 11-pg., 2023-09-30. (20/08421-9, 20/04067-6, 20/03623-2, 19/13496-0, 21/12264-9, 18/14582-5)

MESQUITA, LEONARDO M. DE SOUZA; CONTIERI, LETICIA S.; VAZ, BARBARA M. C.; SENCADAS, VITOR; SOSA, FILIPE H. B.; COUTINHO, JOAO A. P.; ROSTAGNO, MAURICIO A.; VENTURA, SONIA P. M.. Valorization of pomegranate waste through green solvent extraction and biochar production: a zero-waste biorefinery approach. GREEN CHEMISTRY, v. 26, n. 23, p. 18-pg., 2024-10-28. (21/11022-1, 20/08421-9, 20/03623-2, 19/13496-0, 21/11023-8, 18/14582-5)

MORENO, JUVER ANDREY JIMENEZ; MARINHO, LEONARDO DE FREITAS; CONTIERI, LETICIA SANCHES; BARROSO, TIAGO LINHARES CRUZ TABOSA; ROSTAGNO, MAURICIO ARIEL; CARNEIRO, TANIA FORSTER. Obtaining Extracts and Hydrolysates from Cambuci Peel Through Subcritical Water: An In-line Detection Approach. Food and Bioprocess Technology, v. 17, n. 12, p. 20-pg., 2024-05-07. (18/14938-4, 20/03623-2, 22/11690-7, 23/02064-8, 18/14582-5, 23/04479-0)

VAZ, BARBARA M. C.; CONTIERI, LETICIA S.; SOSA, FILIPE H. B.; MARTINS, MARGARIDA; CONDE, ALEXANDRA; DIAS, ANA C. R. V.; ROSTAGNO, MAURICIO A.; MESQUITA, LEONARDO M. DE SOUZA; VENTURA, SONIA P. M.. Unleashing the potential of castor oil as extraction solvent of carotenoids from tomatoes. Separation and Purification Technology, v. 358, p. 10-pg., 2024-10-31. (18/14582-5, 20/08421-9, 20/03623-2)

CONTIERI, LETICIA S.; DE SOUZA MESQUITA, LEONARDO M.; SANCHES, VITOR L.; VIGANO, JULIANE; MARTINEZ, JULIAN; DA CUNHA, DIOGO T.; ROSTAGNO, MAURICIO A.. Standardization proposal to quality control of propolis extracts commercialized in Brazil : A fingerprinting methodology using a UHPLC-PDA-MS/MS approach. Food Research International, v. 161, p. 11-pg., 2022-08-30. (18/14582-5, 19/13496-0, 20/08421-9, 20/03623-2, 20/15774-5)

CONTIERI, LETICIAS.; RIBEIRO, TANIA B.; SOSA, FILIPE H. B.; VAZ, BARBARA M. C.; PIZANI, RODRIGO S.; PINTADO, MANUELA; VENTURA, SONIA P. M.; MESQUITA, LEONARDO M. DE SOUZA; ROSTAGNO, MAURICIO A.. Unlocking the Full Potential of Green Propolis: A Novel Extraction Approach Using Eutectic Solvents for Improved Phenolic Compound Recovery. ACS SUSTAINABLE CHEMISTRY & ENGINEERING, v. 11, n. 36, p. 13-pg., 2023-08-25. (21/11022-1, 18/14582-5, 20/08421-9, 19/13496-0, 20/03623-2, 21/11023-8, 20/04067-6)