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Biosynthesis of roussoellatide, a polyketide isolated from the fungus Roussoella sp. DLM-33

Grant number: 20/11691-8
Support type:Scholarships abroad - Research Internship - Doctorate (Direct)
Effective date (Start): December 01, 2020
Effective date (End): November 30, 2021
Field of knowledge:Physical Sciences and Mathematics - Chemistry - Organic Chemistry
Principal researcher:Roberto Gomes de Souza Berlinck
Grantee:Darlon Irineu Bernardi
Supervisor abroad: Russell John Cox
Home Institution: Instituto de Química de São Carlos (IQSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
Research place: Leibniz Universität Hannover, Germany  
Associated to the scholarship:16/21341-9 - Hydrophilic and bioactive secondary metabolites produced by filamentous fungi, BP.DD


Investigation of Roussoella sp. DLM-33 cultures revealed a new polyketide, roussoellatide,which encompasses an unprecedented carbon skeleton. The biosynthetic pathway proposed for roussoellatide was investigated by feeding experiments with labeled precursors. The hypothetical pathway requires at least two Favorskii-like rearrangements and an intermolecular [4+2]cycloaddition. Such reactions lack biochemical characterization. The principal aim of this proposal is to shed light on the genes encoding Favorskii oxidases and Diels-Alderases using genome mining and genetic engineering. Knock-out mutants will be generated to confirm the association of candidate genes with roussoellatide biosynthesis. A deep investigation of the unique roussoellatide biosynthesis will expand the scope of uncommon biosynthesis enzymes of fungal secondary metabolism. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BERLINCK, ROBERTO G. S.; CRNKOVIC, CAMILA M.; GUBIANI, JULIANA R.; BERNARDI, DARLON I.; IOCA, LAURA P.; QUINTANA-BULLA, JAIRO I. The isolation of water-soluble natural products - challenges, strategies and perspectives. NATURAL PRODUCT REPORTS, OCT 2021. Web of Science Citations: 0.
KAHLERT, LUKAS; BERNARDI, DARLON; HAUSER, MAURICE; IOCA, LAURA P.; BERLINCK, ROBERTO G. S.; SKELLAM, ELIZABETH J.; COX, RUSSELL J. Early Oxidative Transformations During the Biosynthesis of Terrein and Related Natural Products. CHEMISTRY-A EUROPEAN JOURNAL, v. 27, n. 46, p. 11895-11903, AUG 16 2021. Web of Science Citations: 0.

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