Structural investigation of lipid and amphiphilic peptides via Langmuir and Langmuir-Blodgett films

• Grant number: 20/02192-8
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• Start date: February 01, 2021
• End date: January 31, 2022
• Field of knowledge: Biological Sciences - Biophysics - Biophysics of Processes and Systems
• Principal Investigator: Wendel Andrade Alves
• Grantee: Barbara Bianca Gerbelli
• Supervisor: Ian William Hamley
• Host Institution: Centro de Ciências Naturais e Humanas (CCNH). Universidade Federal do ABC (UFABC). Ministério da Educação (Brasil). Santo André , SP, Brazil
• Institution abroad: University of Reading, England
• Associated to the scholarship: 18/05888-3 - Biosensors based on amphiphilic peptides for detection and diagnosis of diseases, BP.PD

Abstract

We propose the synthesis and characterization of new lipopeptide as functional models of the enzyme acetylcholinesterase (AChE) nanostructures obtained from self-assembly and the AChE, which is usually used in pesticide biosensors. For this study, the control of molecular architectures will be explored by using the Langmuir and Langmuir-Blodgett techniques, employing mixtures of unsaturated lipids (such as lecithin/DPPC) and lipopeptides compounds. The choice for these classes relies on the rich polymorphism of mesophases that they can form and, on their ability, to establish bio-interfaces through molecular recognition, which increases their application significantly. Scattering techniques (X-rays and neutrons) will be used to probe structural aspects and provide insights into the mechanisms involved in the self-assembly. Techniques providing complementary information in both direct space will be used, as spectroscopy and microscopy. The combination of these techniques will allow us to correlate the physicochemical mechanisms involved in the building of the nanostructures. In following, structural changes will be evaluated in the peptides by varying the amino acid residues. The goal is to obtain different supramolecular organizations with potential applications as pesticide biosensors. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SOUZA, BRUNA B. S.; LOURENCO, THIAGO C.; GERBELLI, BARBARA B.; OSELIERO FILHO, PEDRO L.; OLIVEIRA, CRISTIANO L. P.; MIRANDA, ANTONIO; DA SILVA, EMERSON R.. A biophysical study of DNA condensation mediated by histones and protamines. JOURNAL OF MOLECULAR LIQUIDS, v. 368, p. 11-pg., 2022-11-11. (20/13204-7, 18/05888-3, 19/12301-1, 20/02192-8)

MARTINEZ, RENATA MILIANI; OSELIERO FILHO, PEDRO LEONIDAS; GERBELLI, BARBARA BIANCA; MAGALHAES, WAGNER VIDAL; VELASCO, MARIA VALERIA ROBLES; DA SILVA LANNES, SUZANA CAETANO; DE OLIVEIRA, CRISTIANO LUIS PINTO; ROSADO, CATARINA; BABY, ANDRE ROLIM. Influence of the Mixtures of Vegetable Oil and Vitamin E over the Microstructure and Rheology of Organogels. GELS, v. 8, n. 1, JAN 2022. (16/24531-3, 20/02192-8, 19/16169-0, 19/12301-1, 18/05888-3, 20/13204-7)

GERBELLI, BARBARA B.; FILHO, PEDRO L. O.; CORTEZ, BRUNA; SODRE, PEDRO T.; COUTINHO-NETO, MAURICIO D.; HAMLEY, IAN W.; SEITSONEN, JANI; ALVES, WENDEL A.. Interaction between glyphosate pesticide and amphiphilic peptides for colorimetric analysis. NANOSCALE ADVANCES, v. 4, n. 17, p. 8-pg., 2022-07-28. (14/50867-3, 17/02317-2, 20/13204-7, 18/05888-3, 19/12301-1, 20/02192-8)