Quantification and Determination of Composition of Steryl Ferulates in Refined Rice Bran Oils Using an UPLC-MS Method

Author(s)

Cuevas, Maite S. ; de Souza, Patricia T. ; da Costa Rodrigues, Christianne E. ; Meirelles, Antonio J. A.

• Document type: Journal article
• Source: JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY; v. 94, n. 3, p. 375-385, MAR 2017.
• Web of Science Citations: 1

Abstract

Gamma-oryzanol contains a mixture of steryl ferulates found in rice bran oil. Several studies have attributed nutraceutical properties to this mixture, such as hypocholesterolemic and anti-inflammatory activities. A method based on ultra-performance liquid chromatography coupled with electrospray ionization mass spectrometry was developed and evaluated for the simultaneous quantification of gamma-oryzanol and identification of five major steryl ferulates directly in refined rice bran oils (RBO) samples. The proposed method was evaluated according to linearity by obtaining standard curves with R (2) values above 0.990, and limit of detection values ranged from 1.9 to 5.9 A mu g/mL, whereas limits of quantification ranged from 5.9 to 17.9 A mu g/mL; inter- and intraday accuracy and precision were within the range required by the US Food and Drug Administration guidelines; recovery levels ranged from 78 to 85% for gamma-oryzanol, and from 84 to 119% for steryl ferulates. The method can be considered robust in relation to the NH4OH (ammonium hydroxide) content and cone voltage variations, with coefficient of variation and average relative percentage deviation values lower than 7.0 and 4.4%, respectively. The stability during the storage test was maintained in concentrated samples (18.5 A mu g/mL), with recovered values between 93 and 113%. This method was successfully applied to the analysis of RBO samples, demonstrating that it could be easily used for quality control purposes. (AU)

• FAPESP's process: 14/09446-4 - Development of a process of vegetable oil extraction using alcoholic solvents: evaluation of oil composition and changes in the protein fraction
• Grantee: Christianne Elisabete da Costa Rodrigues
• Support type: Regular Research Grants
• FAPESP's process: 14/21252-0 - Equilibrium and production processes of biofuels and bioproducts
• Grantee: Antonio José de Almeida Meirelles
• Support type: Research Projects - Thematic Grants
• FAPESP's process: 09/54137-1 - Acquisition of a particle size/distribution analyzer, a zeta potential measurement system, a spray dryer, and an ultra-high-pressure liquid chromatograph/mass spectrometer
• Grantee: Miriam Dupas Hubinger
• Support type: Multi-user Equipment Program
• FAPESP's process: 08/56258-8 - Phase equilibrium and purification processes in the production of biofuels and biocompounds
• Grantee: Antonio José de Almeida Meirelles
• Support type: Program for Research on Bioenergy (BIOEN) - Thematic Grants
• FAPESP's process: 15/07370-3 - Preservation of Carotenoids in Oil from Palm Pressed Fiber extracted using Hexane + Alcohol Mixtures as Solvents
• Grantee: Maitê Sarria Cuevas
• Support type: Scholarships in Brazil - Post-Doctorate