Combining pressurized liquids with ultrasound to improve the extraction of phenolic compounds from pomegranate peel (Punica granatum L.)

Author(s)

Sumere, Beatriz Rocchetti ^[1] ; de Souza, Mariana Correa ^[1] ; dos Santos, Mariana Pacifico ^[1] ; Neves Bezerra, Rosangela Maria ^[1] ; da Cunha, Diogo Thimoteo ^[1] ; Martinez, Julian ^[2] ; Rostagno, Mauricio Ariel ^[1]

Affiliation

^[1] Univ Campinas UNICAMP, Sch Appl Sci FCA, Lab Funct Properties Foods LAPFAL, Sao Paulo - Brazil

^[2] Univ Estadual Campinas, Coll Food Engn FEA, Dept Food Engn, Lab High Pressure Food Engn LAPEA, Sao Paulo - Brazil

• Document type: Journal article
• Source: ULTRASONICS SONOCHEMISTRY; v. 48, p. 151-162, NOV 2018.
• Web of Science Citations: 13

Abstract

The combination of ultrasound and pressurized liquid extraction (UAPLE) was evaluated for the extraction of phenolic compounds from pomegranate peels (Punica granatunt L.). The influence of several variables of the process on extraction yield, including solvent type (water, ethanol + water 30, 50 and 70% v:v), temperature (50-100 degrees C), ultrasound power (0-800 W at the generator, or 0-38.5 W at the tip of the probe), mean particle size (0.68 and 1.05 mm), and number of cycles (1-5), were analyzed according to the yield of 20 different phenolic compounds. The most suitable temperatures for the extraction of phenolic compounds using water were from 70 to 80 degrees C. In general, 100 degrees C was not adequate since the lowest extraction yields were observed. Results suggested that ultrasound had a greater impact on extraction yields using large particles and that intermediate ultrasound power (480-640 W at the generator, or 23.1-30.8 W at the tip of the probe) produced the best results. Using small particles (0.68 mm) or large particles (1.05 mm), extraction with ultrasound was 1 cycle faster. Ultrasound may have offset the negative effect of the use of large particles, however, did not increase the yield of phenolic compounds in any of the cases studied after five cycles. Additionally, the continuous clogging problems observed with small particles were avoided with the use of large particles, which combined with ultrasound allowed consistent operation with good intra and inter-day reproducibility (> 95%). Using samples with large particle size, the best extraction conditions were achieved with water extraction solvent, 70 degrees C extraction temperature, ultrasound power at 480 W, and 3 cycles, yielding 61.72 +/- 7.70 mg/g. UAPLE demonstrated to be a clean, efficient and a green alternative for the extraction of phenolic compounds from pomegranate peels. These findings indicate that UAPLE has a great potential to improve the extraction of bioactive compounds from natural products. (AU)

• FAPESP's process: 16/19930-6 - Evaluation of the extraction with pressurized liquid assisted by ultrasound coupled to the solid phase extraction for the purification of phenolic compounds from tea (Camellia sinensis)
• Grantee: Mariana Corrêa de Souza
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 15/11932-7 - Integration of high pressure extraction to separation operations for the recovery of by-products from fruit processing
• Grantee: Julian Martínez
• Support Opportunities: Regular Research Grants
• FAPESP's process: 16/06457-0 - Leaves and stalks of beet (Beta vulgaris L.): characterization and effects of the antioxidant capacity in obese and dyslipidemic
• Grantee: Rosângela Maria Neves Bezerra
• Support Opportunities: Regular Research Grants
• FAPESP's process: 16/20016-7 - Extraction of bioactive compounds from husks of pomegranate (Punica granatum L.) using pressurized liquids assisted by ultrasound
• Grantee: Mariana Pacífico dos Santos
• Support Opportunities: Scholarships in Brazil - Master