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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Genetic diversity, virulence genotype and antimicrobial resistance of uropathogenic Escherichia coli (UPEC) isolated from sows

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Author(s):
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Spindola, Maria G. [1] ; Cunha, Marcos P. V. [1] ; Moreno, Luisa Z. [1] ; Amigo, Cristina R. [1] ; Silva, Ana P. S. [1] ; Parra, Beatriz M. [1] ; Poor, Andre P. [1] ; de Oliveira, Carolina H. [1] ; Perez, Barbara P. [1] ; Knobl, Terezinha [1] ; Moreno, Andrea M. [1]
Total Authors: 11
Affiliation:
[1] Univ Sao Paulo, Fac Med Vet & Zootecnia, Sao Paulo - Brazil
Total Affiliations: 1
Document type: Journal article
Source: VETERINARY QUARTERLY; v. 38, n. 1, p. 79-87, OCT 26 2018.
Web of Science Citations: 0
Abstract

Background: Urinary tract infections (UTI) cause severe losses to the swine industry worldwide and uropathogenic Escherichia coli (UPEC) are the main agent isolated from UTI in sows.Objective: The aim of this study was to investigate the virulence genes, assess the phylogenetic background, clonal diversity, and the pattern of resistance to antimicrobials in 186 isolates of UPEC isolated from sows in Brazil.Materials and methods: Urine samples from 300 sows of three herds with clinical signs from SAo Paulo State (Brazil) were screened for UTI; samples with suggestive results were submitted to bacterial isolation. E. coli strains isolated were characterized using disk diffusion technique, polymerase chain reaction and Single-enzyme amplification fragment length polymorphism (SE-AFLP).Results: Virulence genes focH and papC were present in 78.5% and 58% of strains, respectively, followed by cnf1 (23.2%), afa (13.4%), sfa (11.3%), iucD (6.9%), and hlyA (1.6%). No clonal relatedness was found by SE-AFLP. A total of 98% of isolates (182/186) were multidrug resistant, and the highest levels of resistance were to sulfonamides, tetracycline, florfenicol, and ampicillin. Isolates were classified in phylogenetic group B1 (34.4%), followed by D (33.9%), E (30.1%) and A (1.6%).Conclusions: The data obtained suggest that pigs from clinically affected herds may serve as a reservoir of uropathogenic and multidrug-resistant E. coli strains. (AU)

FAPESP's process: 13/17136-2 - Characterization of Leptospira strains isolated in Brazil by pulsed field gel electrophoresis (PFGE) typing and comparative genomic analysis.
Grantee:Luisa Zanolli Moreno
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 13/16946-0 - Phenotypic and genotypic characterization of strains of Salmonella enterica isolated from pork in São Paulo.
Grantee:Vasco Túlio de Moura Gomes
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 14/11523-7 - Characterization of plasmid-mediated quinolone resistance genes in Escherichia coli isolated from poultry and swine production chain
Grantee:Marcos Paulo Vieira Cunha
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 16/25745-7 - Virulence assessment and phenotypic and genomic characterization of Corynebacterium spp. isolated from sow's urinary tract infection
Grantee:Luisa Zanolli Moreno
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 17/09515-4 - Identification and phenotypic characterization of bacterial pathogens causing metritis in sows.
Grantee:André Pegoraro Poor
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 11/22608-5 - Urinary tract infections caused by uropatogenic Escherichia coli in sows in São Paulo state
Grantee:Maria Garcia Spindola
Support Opportunities: Scholarships in Brazil - Scientific Initiation