| Full text | |
| Author(s): |
Barbosa, Camila Gouveia
[1]
;
La-Scalea, Mauro Aquiles
[2]
;
Caseli, Luciano
[1]
;
Peres, Laura Oliveira
[1]
Total Authors: 4
|
| Affiliation: | [1] Univ Fed Sao Paulo, Chem Dept, Hybrid Mat Lab, Diadema, SP - Brazil
[2] Univ Fed Sao Paulo, Chem Dept, Electroanalyt & Electrochem Lab, Diadema, SP - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | COLLOID AND INTERFACE SCIENCE COMMUNICATIONS; v. 27, p. 40-44, NOV 2018. |
| Web of Science Citations: | 1 |
| Abstract | |
This paper describes Langmuir monolayers of the polymer poly {[}(9,9-dioctylfluorene)-co-thiophene] (PDOF-co-Th) serving as matrix for urease incorporation and transferred to platinum supports as Langmuir-Blodgett films. The supramolecular structure forming highly organized films not only tuned the catalytic activity of urease, but also helped to prove the viability of the system to sensing urea as demonstrated with current-voltage and constant voltage measurements, exhibiting regular and distinctive output signals over all concentrations (from 1 x 10(-3) to 1.5 x 10(-2) mol/L) as demonstrated in this work. These results are related to the synergism between the compounds on the active layer, leading to a surface molecular arrangement which allowed fast analyte diffusion owing to an adequate molecular accommodation, taking advantage of the urease activity and of the properties of the polymer as molecular accommodator. This work then demonstrates the feasibility of employing LB films composed of copolymers and urease as electrochemical devices for biosensing applications as a proof-ofconcept experiment. (AU) | |
| FAPESP's process: | 14/50869-6 - INCT 2014: on Organic Electronics |
| Grantee: | Roberto Mendonça Faria |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 15/23446-0 - Smart surfaces: nanostructured and bioinspired systems for the investigation of molecular interactions in membrane models and for the production of devices for biosensors and environmental analysis |
| Grantee: | Luciano Caseli |
| Support Opportunities: | Regular Research Grants |