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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Immobilization of urease in Langmuir-Blodgett films of di-ureasil hybrid compounds

Full text
Author(s):
Teles Rossini, Caio Vinicius [1] ; Molina, Celso [1] ; Caseli, Luciano [1]
Total Authors: 3
Affiliation:
[1] Fed Univ Sao Paulo UNIFESP, Dept Chem, Diadema, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Thin Solid Films; v. 670, p. 17-23, JAN 31 2019.
Web of Science Citations: 1
Abstract

Incorporation of enzymes in matrices as nanostructured films is a strategy to achieve molecular architectures able to enhance the efficiency of sensors and optoelectronic devices. In this present work, urease was inserted in the aqueous subphase of a di-ureasil hybrid compound (DUPTS) assembled as a Langmuir monolayer. Phosphotungstic acid (PWA) was introduced in the aqueous subphase in order to stabilize DUPTS by means of electrostatic interactions. Incorporation of urease in the Langmuir film could be attested with surface pressure and surface potential-area isotherms, rheological measurements, polarization modulation infrared reflection-absorption spectroscopy, and Brewster angle microscopy. The monolayers were transferred to solid supports as Langmuir-Blodgett films and characterized with infrared and fluorescence spectroscopies, proving not only the co-transfer of urease to the solid support, but also the conservation of part of its secondary structure. The enzyme activity could be detected towards urea hydrolysis enabling the urease/PWA/DUPTS supramolecular system to be employed for sensors as well as for other optoelectronic devices. (AU)

FAPESP's process: 15/23446-0 - Smart surfaces: nanostructured and bioinspired systems for the investigation of molecular interactions in membrane models and for the production of devices for biosensors and environmental analysis
Grantee:Luciano Caseli
Support Opportunities: Regular Research Grants
FAPESP's process: 13/14262-7 - Nanostructured films from biologically-relevant materials
Grantee:Osvaldo Novais de Oliveira Junior
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 14/12557-2 - Multi-user equipment approved in grant 2013/14262-7: interfacial reometer
Grantee:Osvaldo Novais de Oliveira Junior
Support Opportunities: Multi-user Equipment Program